ID   MEP1_MAGO7              Reviewed;         283 AA.
AC   A4RGT4; G4MXP9;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Extracellular metalloprotease MGG_08041;
DE            EC=3.4.24.-;
DE   Flags: Precursor;
GN   ORFNames=MGG_08041;
OS   Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS   fungus) (Pyricularia oryzae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX   NCBI_TaxID=242507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX   PubMed=15846337; DOI=10.1038/nature03449;
RA   Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA   Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA   Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA   Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA   Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA   Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT   "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL   Nature 434:980-986(2005).
CC   -!- FUNCTION: Secreted metalloproteinase that allows assimilation of
CC       proteinaceous substrates. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase M43B family. {ECO:0000305}.
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DR   EMBL; CM001232; EHA55186.1; -; Genomic_DNA.
DR   RefSeq; XP_003714993.1; XM_003714945.1.
DR   AlphaFoldDB; A4RGT4; -.
DR   SMR; A4RGT4; -.
DR   STRING; 318829.MGG_08041T0; -.
DR   MEROPS; M43.008; -.
DR   EnsemblFungi; MGG_08041T0; MGG_08041T0; MGG_08041.
DR   GeneID; 2678331; -.
DR   KEGG; mgr:MGG_08041; -.
DR   VEuPathDB; FungiDB:MGG_08041; -.
DR   eggNOG; ENOG502QQ7Z; Eukaryota.
DR   HOGENOM; CLU_048726_0_0_1; -.
DR   InParanoid; A4RGT4; -.
DR   OMA; INETWAN; -.
DR   OrthoDB; 1569419at2759; -.
DR   Proteomes; UP000009058; Chromosome 2.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd04275; ZnMc_pappalysin_like; 1.
DR   Gene3D; 3.40.390.10; -; 1.
DR   InterPro; IPR024079; MetalloPept_cat_dom_sf.
DR   InterPro; IPR008754; Peptidase_M43.
DR   Pfam; PF05572; Peptidase_M43; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Glycoprotein; Hydrolase; Metal-binding; Metalloprotease;
KW   Protease; Reference proteome; Secreted; Signal; Zinc.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..283
FT                   /note="Extracellular metalloprotease MGG_08041"
FT                   /id="PRO_0000407202"
FT   ACT_SITE        198
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         197
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         201
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   CARBOHYD        55
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        233..260
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   283 AA;  29953 MW;  241FB7E442A690BA CRC64;
     MQINVVKTFL FALAASSVSA LAVDTEFRCG APEPSEELIE ASAIMAVAEA EAAANGTLAA
     RQSALTIDTY VHVVATSTSA SAGYLSDATI QQQLRVMNED YAPSGIQFVL KGTDRTVNAN
     WARDSGETAM KTALRKGTYK DLNLYFLSSI PGGILGYCYF PASATTSTVR LDGCTIASGT
     VPGGSISRFN LGKTAVHEVG HWFGLYHTFQ GGCNGQGDLV DDTPAQASAS SGCPIGRDSC
     PNQPGLDPIH NYMDYSDDSC YEEFTPGQNA RMSSMFAQFR AGK