MEOX1_DANRE
ID MEOX1_DANRE Reviewed; 253 AA.
AC F1Q4R9; Q6DHF3;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=Homeobox protein MOX-1 {ECO:0000250|UniProtKB:P50221};
DE AltName: Full=Mesenchyme homeobox 1 {ECO:0000250|UniProtKB:P50221};
DE AltName: Full=Protein choker {ECO:0000303|PubMed:25119043};
GN Name=meox1 {ECO:0000312|ZFIN:ZDB-GENE-040718-149};
GN Synonyms=cho {ECO:0000303|PubMed:25119043};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000312|Ensembl:ENSDARP00000096438};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RX PubMed=25119043; DOI=10.1038/nature13678;
RA Nguyen P.D., Hollway G.E., Sonntag C., Miles L.B., Hall T.E., Berger S.,
RA Fernandez K.J., Gurevich D.B., Cole N.J., Alaei S., Ramialison M.,
RA Sutherland R.L., Polo J.M., Lieschke G.J., Currie P.D.;
RT "Haematopoietic stem cell induction by somite-derived endothelial cells
RT controlled by meox1.";
RL Nature 512:314-318(2014).
CC -!- FUNCTION: Mesodermal transcription factor that plays a key role in
CC somitogenesis and is specifically required for sclerotome development.
CC Required for maintenance of the sclerotome polarity and formation of
CC the cranio-cervical joints. Binds specifically to the promoter of
CC target genes and regulates their expression. Required for hematopoietic
CC stem cell (HSCs) induction via its role in somitogenesis: specification
CC of HSCs occurs via the deployment of a specific endothelial precursor
CC population, which arises within a sub-compartment of the somite named
CC endotome. Acts by mediating specification of endothelial cells of the
CC endotome within the nascent somite, notably by repressing expression of
CC cxcl12b. {ECO:0000269|PubMed:25119043}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P32442}. Cytoplasm
CC {ECO:0000250|UniProtKB:P32442}. Note=Localizes predominantly in the
CC nucleus. {ECO:0000250|UniProtKB:P32442}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F1Q4R9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F1Q4R9-2; Sequence=VSP_057099;
CC -!- DEVELOPMENTAL STAGE: Expressed within the early somite and then becomes
CC restricted to the external cell layer (ECL) and consequently to
CC appendicular muscle populations. {ECO:0000269|PubMed:25119043}.
CC -!- DISRUPTION PHENOTYPE: Defects in somite lineages. Secondary trunk
CC myogenesis is reduced, as well as appendicular and hypaxial muscles and
CC their progenitors. These cell types derive from the external cell layer
CC (ECL) and ECL cell numbers are reduced. The endotome is expanded at the
CC expense of a second somitic cell type. The resulting increase in
CC endotome-derived cells that migrate to colonize the dorsal aorta
CC generates a dramatic increase in chemokine-dependent hematopoietic stem
CC cell (HSCs) induction. {ECO:0000269|PubMed:25119043}.
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DR EMBL; CR847827; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC076021; AAH76021.1; -; mRNA.
DR RefSeq; NP_001002450.2; NM_001002450.2. [F1Q4R9-1]
DR AlphaFoldDB; F1Q4R9; -.
DR SMR; F1Q4R9; -.
DR STRING; 7955.ENSDARP00000096438; -.
DR PaxDb; F1Q4R9; -.
DR Ensembl; ENSDART00000011252; ENSDARP00000027691; ENSDARG00000115382. [F1Q4R9-1]
DR Ensembl; ENSDART00000105661; ENSDARP00000096438; ENSDARG00000007891. [F1Q4R9-1]
DR GeneID; 436723; -.
DR KEGG; dre:436723; -.
DR CTD; 4222; -.
DR ZFIN; ZDB-GENE-040718-149; meox1.
DR eggNOG; KOG0489; Eukaryota.
DR GeneTree; ENSGT00940000154018; -.
DR HOGENOM; CLU_081326_1_0_1; -.
DR InParanoid; F1Q4R9; -.
DR OMA; CMRSPQP; -.
DR OrthoDB; 1522857at2759; -.
DR PhylomeDB; F1Q4R9; -.
DR TreeFam; TF351603; -.
DR PRO; PR:F1Q4R9; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 12.
DR Bgee; ENSDARG00000007891; Expressed in muscle tissue and 8 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:ZFIN.
DR GO; GO:0003682; F:chromatin binding; IDA:ZFIN.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0048066; P:developmental pigmentation; IMP:ZFIN.
DR GO; GO:0060218; P:hematopoietic stem cell differentiation; IMP:UniProtKB.
DR GO; GO:0030097; P:hemopoiesis; IMP:ZFIN.
DR GO; GO:0055001; P:muscle cell development; IMP:ZFIN.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0061056; P:sclerotome development; ISS:UniProtKB.
DR GO; GO:0001501; P:skeletal system development; IMP:ZFIN.
DR GO; GO:0061053; P:somite development; IMP:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR042634; MOX-1/MOX-2.
DR PANTHER; PTHR24328; PTHR24328; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; Cytoplasm; Developmental protein;
KW DNA-binding; Homeobox; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..253
FT /note="Homeobox protein MOX-1"
FT /id="PRO_0000430816"
FT DNA_BIND 170..229
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 136..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 226..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 143..158
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 15..27
FT /note="Missing (in isoform 2)"
FT /id="VSP_057099"
SQ SEQUENCE 253 AA; 28150 MW; 36F788BDB2597576 CRC64;
MEQSASSCMR SPHTGGALWG CVRSPHSGGS GAGIQPYQQA PFALHQKHDF LAYTDFSSSC
LVPAPHAYPR EDRLYPETHS GYQRTEWQFS PCEPRGRGQE PCQGAAEAVG AEMDSAGGDR
LAGAVTGCLE GDYSPQSVPA VDTEKKSSKR KREVTDIQDS SFKADSNCKA RKERTAFTKE
QLRELEAEFT HHNYLTRLRR YEIAVNLDLT ERQVKVWFQN RRMKWKRVKG GQPASPHDLE
ADELDSAASP SSE
