MENG_ARATH
ID MENG_ARATH Reviewed; 261 AA.
AC Q3ED65; Q6ID15; Q9LQD5; Q9LR22;
DT 05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2006, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic {ECO:0000255|HAMAP-Rule:MF_03192};
DE EC=2.1.1.329 {ECO:0000255|HAMAP-Rule:MF_03192};
DE AltName: Full=Demethylphylloquinone methyltransferase {ECO:0000255|HAMAP-Rule:MF_03192};
DE AltName: Full=Menaquinone biosynthesis methyltransferase ubiE-like protein {ECO:0000255|HAMAP-Rule:MF_03192};
DE Flags: Precursor;
GN Name=MENG {ECO:0000255|HAMAP-Rule:MF_03192}; OrderedLocusNames=At1g23360;
GN ORFNames=F26F24.24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=17082184; DOI=10.1074/jbc.m609412200;
RA Lohmann A., Schoettler M.A., Brehelin C., Kessler F., Bock R., Cahoon E.B.,
RA Doermann P.;
RT "Deficiency in phylloquinone (vitamin K1) methylation affects prenyl
RT quinone distribution, photosystem I abundance, and anthocyanin accumulation
RT in the Arabidopsis AtmenG mutant.";
RL J. Biol. Chem. 281:40461-40472(2006).
CC -!- FUNCTION: Involved in the biosynthesis of phylloquinone (vitamin K1).
CC Methyltransferase required for the conversion of 2-phytyl-1,4-beta-
CC naphthoquinol to phylloquinol. {ECO:0000255|HAMAP-Rule:MF_03192,
CC ECO:0000269|PubMed:17082184}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=demethylphylloquinol + S-adenosyl-L-methionine = H(+) +
CC phylloquinol + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:40551,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:28433, ChEBI:CHEBI:57856,
CC ChEBI:CHEBI:59789, ChEBI:CHEBI:87844; EC=2.1.1.329;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_03192,
CC ECO:0000269|PubMed:17082184};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_03192, ECO:0000269|PubMed:17082184}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3ED65-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3ED65-2; Sequence=VSP_040914;
CC -!- DISRUPTION PHENOTYPE: Lack of phylloquinone and reduced growth under
CC normal light. Loss of anthocyanin accumulation under high light.
CC {ECO:0000269|PubMed:17082184}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. MenG/UbiE family. {ECO:0000255|HAMAP-Rule:MF_03192}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79599.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF87008.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005292; AAF87008.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC007945; AAF79599.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30377.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30378.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30379.1; -; Genomic_DNA.
DR EMBL; BT014858; AAT41841.1; -; mRNA.
DR EMBL; AK317221; BAH19903.1; -; mRNA.
DR PIR; F86367; F86367.
DR RefSeq; NP_173750.3; NM_102185.4. [Q3ED65-1]
DR RefSeq; NP_973894.1; NM_202165.1. [Q3ED65-2]
DR RefSeq; NP_973895.1; NM_202166.2. [Q3ED65-2]
DR AlphaFoldDB; Q3ED65; -.
DR SMR; Q3ED65; -.
DR STRING; 3702.AT1G23360.1; -.
DR iPTMnet; Q3ED65; -.
DR PaxDb; Q3ED65; -.
DR PRIDE; Q3ED65; -.
DR ProteomicsDB; 232229; -. [Q3ED65-1]
DR EnsemblPlants; AT1G23360.1; AT1G23360.1; AT1G23360. [Q3ED65-1]
DR EnsemblPlants; AT1G23360.2; AT1G23360.2; AT1G23360. [Q3ED65-2]
DR EnsemblPlants; AT1G23360.3; AT1G23360.3; AT1G23360. [Q3ED65-2]
DR GeneID; 838945; -.
DR Gramene; AT1G23360.1; AT1G23360.1; AT1G23360. [Q3ED65-1]
DR Gramene; AT1G23360.2; AT1G23360.2; AT1G23360. [Q3ED65-2]
DR Gramene; AT1G23360.3; AT1G23360.3; AT1G23360. [Q3ED65-2]
DR KEGG; ath:AT1G23360; -.
DR Araport; AT1G23360; -.
DR TAIR; locus:2028050; AT1G23360.
DR eggNOG; KOG1540; Eukaryota.
DR HOGENOM; CLU_037990_0_0_1; -.
DR InParanoid; Q3ED65; -.
DR OMA; WQKSALN; -.
DR PhylomeDB; Q3ED65; -.
DR BioCyc; ARA:AT1G23360-MON; -.
DR BRENDA; 2.1.1.329; 399.
DR PRO; PR:Q3ED65; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q3ED65; baseline and differential.
DR Genevisible; Q3ED65; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0052624; F:2-phytyl-1,4-naphthoquinone methyltransferase activity; IMP:TAIR.
DR GO; GO:0008168; F:methyltransferase activity; IBA:GO_Central.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0042372; P:phylloquinone biosynthetic process; IMP:TAIR.
DR GO; GO:0006744; P:ubiquinone biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.40.50.150; -; 1.
DR HAMAP; MF_01982; MenG_phylloquinone_subfam; 1.
DR HAMAP; MF_01813; MenG_UbiE_methyltr; 1.
DR InterPro; IPR032904; MenG.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR004033; UbiE/COQ5_MeTrFase.
DR InterPro; IPR023576; UbiE/COQ5_MeTrFase_CS.
DR Pfam; PF01209; Ubie_methyltran; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR TIGRFAMs; TIGR01934; MenG_MenH_UbiE; 1.
DR PROSITE; PS51608; SAM_MT_UBIE; 1.
DR PROSITE; PS01183; UBIE_1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; Methyltransferase; Plastid;
KW Reference proteome; S-adenosyl-L-methionine; Transferase; Transit peptide.
FT TRANSIT 1..30
FT /note="Chloroplast"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03192"
FT CHAIN 31..261
FT /note="2-phytyl-1,4-beta-naphthoquinone methyltransferase,
FT chloroplastic"
FT /id="PRO_0000406986"
FT VAR_SEQ 1..101
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_040914"
SQ SEQUENCE 261 AA; 29051 MW; 933A722FD246F1EE CRC64;
MAALLGIVSP VTFTGKHPVN SRSRRRTVVK CSNERRILFN RIAPVYDNLN DLLSLGQHRI
WKNMAVSWSG AKKGDYVLDL CCGSGDLAFL LSEKVGSTGK VMGLDFSSEQ LAVAATRQSL
KARSCYKCIE WIEGDAIDLP FDDCEFDAVT MGYGLRNVVD RLRAMKEMYR VLKPGSRVSI
LDFNKSNQSV TTFMQGWMID NVVVPVATVY DLAKEYEYLK YSINGYLTGE ELETLALEAG
FSSACHYEIS GGFMGNLVAM R
