ID   MED8_ASPCL              Reviewed;         254 AA.
AC   A1CHE9;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 8;
DE   AltName: Full=Mediator complex subunit 8;
GN   Name=med8; ORFNames=ACLA_047730;
OS   Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 /
OS   NRRL 1 / QM 1276 / 107).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=344612;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA   Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA   Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA   Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA   Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA   White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA   Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC       {ECO:0000305}.
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DR   EMBL; DS027054; EAW10304.1; -; Genomic_DNA.
DR   RefSeq; XP_001271730.1; XM_001271729.1.
DR   AlphaFoldDB; A1CHE9; -.
DR   SMR; A1CHE9; -.
DR   STRING; 5057.CADACLAP00004395; -.
DR   EnsemblFungi; EAW10304; EAW10304; ACLA_047730.
DR   GeneID; 4704270; -.
DR   KEGG; act:ACLA_047730; -.
DR   VEuPathDB; FungiDB:ACLA_047730; -.
DR   eggNOG; ENOG502S8U1; Eukaryota.
DR   HOGENOM; CLU_074399_1_0_1; -.
DR   OMA; WAPIEAN; -.
DR   OrthoDB; 1472120at2759; -.
DR   Proteomes; UP000006701; Unassembled WGS sequence.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR019364; Mediatior_Med8_fun/met.
DR   PANTHER; PTHR13074; PTHR13074; 1.
DR   Pfam; PF10232; Med8; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..254
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   8"
FT                   /id="PRO_0000304534"
FT   REGION          167..234
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          154..190
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        175..197
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   254 AA;  28132 MW;  16F269F7A02B14DA CRC64;
     MASPSQDQIK VLEQSRQRLV QLTRSLGSLI GSLNQSDPLP SWSSLQSQAS IISNNLLSVS
     EHLSDNCDLL SALVAYPGPE YPGRTQASTL EQLLRTKLDP RIEDWVARGR RAGASALEDK
     DALSETELAE LWDWAPVEAN QEARRRNWGG DYTLEEREMG IQNVVTGLRR QLEDDERDED
     EDEDDEEEEE GEGEDEEMEV VGVRRRSDAG AGLGFDTAVP TPASSQQVQK GAGPVVPLDD
     VLRFMTTGTL PQQR