ID   MCFS_DICDI              Reviewed;         285 AA.
AC   Q54FE6;
DT   13-OCT-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Mitochondrial substrate carrier family protein S;
DE   AltName: Full=Carnitine/acylcarnitine translocase;
DE            Short=CAC;
DE   AltName: Full=Solute carrier family 25 member 20 homolog B;
GN   Name=mcfS; Synonyms=slc25a20B; ORFNames=DDB_G0290913;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   REVIEW.
RX   PubMed=17442478; DOI=10.1016/j.biochi.2007.03.004;
RA   Satre M., Mattei S., Aubry L., Gaudet P., Pelosi L., Brandolin G.,
RA   Klein G.;
RT   "Mitochondrial carrier family: repertoire and peculiarities of the cellular
RT   slime mould Dictyostelium discoideum.";
RL   Biochimie 89:1058-1069(2007).
CC   -!- FUNCTION: Mitochondrial solute carriers shuttle metabolites,
CC       nucleotides, and cofactors through the mitochondrial inner membrane.
CC       Mediates the transport of acylcarnitines of different length across the
CC       mitochondrial inner membrane from the cytosol to the mitochondrial
CC       matrix for their oxidation by the mitochondrial fatty acid-oxidation
CC       pathway (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000172; EAL61972.1; -; Genomic_DNA.
DR   RefSeq; XP_635476.1; XM_630384.1.
DR   AlphaFoldDB; Q54FE6; -.
DR   SMR; Q54FE6; -.
DR   STRING; 44689.DDB0234097; -.
DR   PaxDb; Q54FE6; -.
DR   EnsemblProtists; EAL61972; EAL61972; DDB_G0290913.
DR   GeneID; 8627892; -.
DR   KEGG; ddi:DDB_G0290913; -.
DR   dictyBase; DDB_G0290913; mcfS.
DR   eggNOG; KOG0758; Eukaryota.
DR   HOGENOM; CLU_015166_16_3_1; -.
DR   InParanoid; Q54FE6; -.
DR   OMA; PIDCFRQ; -.
DR   PhylomeDB; Q54FE6; -.
DR   Reactome; R-DDI-70635; Urea cycle.
DR   PRO; PR:Q54FE6; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0000064; F:L-ornithine transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:1990575; P:mitochondrial L-ornithine transmembrane transport; IBA:GO_Central.
DR   Gene3D; 1.50.40.10; -; 1.
DR   InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR   InterPro; IPR023395; Mt_carrier_dom_sf.
DR   Pfam; PF00153; Mito_carr; 3.
DR   SUPFAM; SSF103506; SSF103506; 1.
DR   PROSITE; PS50920; SOLCAR; 3.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW   Repeat; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..285
FT                   /note="Mitochondrial substrate carrier family protein S"
FT                   /id="PRO_0000385526"
FT   TOPO_DOM        1..9
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        10..30
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        31..61
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        62..82
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        83..101
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        102..122
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        123..150
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        151..172
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        173..199
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        200..220
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        221..258
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        259..277
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        278..285
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000250"
FT   REPEAT          4..87
FT                   /note="Solcar 1"
FT   REPEAT          96..183
FT                   /note="Solcar 2"
FT   REPEAT          197..283
FT                   /note="Solcar 3"
SQ   SEQUENCE   285 AA;  30869 MW;  1B556CA74591B004 CRC64;
     MSTERGLKDS IAGTVAGAAC LFTGHPFDTI RVRLQTSNTP IGIMECFRNT IKYEGFSGLY
     KGVTSPLFGM MFETAVLFAG YGQMKVLLQK DENTPLTVGQ CAIAGGFAGV GASVVLTPVE
     LVKCRLQVQT TGPQKYKGSL DCLVQILKEG GIRGAYRGFT PTIAREFVGN MAFFSTYETC
     KRYFKNKENK PNDDDELNLP ALIISGGLGG MAYWTVLYPV DVAKSKIQIS EGAGPSPSIV
     KVLKEIYSKE GVKGLFRGYT PTIIRSFPAN AAMFSVYELV IKLLG