ID   MCAT_CLOAB              Reviewed;         225 AA.
AC   Q97FE0;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Probable manganese catalase;
DE            EC=1.11.1.6;
GN   OrderedLocusNames=CA_C2800;
OS   Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710
OS   / VKM B-1787).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=272562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX   PubMed=11466286; DOI=10.1128/jb.183.16.4823-4838.2001;
RA   Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R.,
RA   Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F.,
RA   Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V.,
RA   Smith D.R.;
RT   "Genome sequence and comparative analysis of the solvent-producing
RT   bacterium Clostridium acetobutylicum.";
RL   J. Bacteriol. 183:4823-4838(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H2O2 = 2 H2O + O2; Xref=Rhea:RHEA:20309, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:16240; EC=1.11.1.6;
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 2 manganese ions per subunit. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC       Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the manganese catalase family. {ECO:0000305}.
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DR   EMBL; AE001437; AAK80744.1; -; Genomic_DNA.
DR   PIR; E97244; E97244.
DR   RefSeq; NP_349404.1; NC_003030.1.
DR   RefSeq; WP_010966085.1; NC_003030.1.
DR   AlphaFoldDB; Q97FE0; -.
DR   SMR; Q97FE0; -.
DR   STRING; 272562.CA_C2800; -.
DR   PeroxiBase; 6192; CacMnCat.
DR   EnsemblBacteria; AAK80744; AAK80744; CA_C2800.
DR   GeneID; 44999285; -.
DR   KEGG; cac:CA_C2800; -.
DR   PATRIC; fig|272562.8.peg.2987; -.
DR   eggNOG; COG3546; Bacteria.
DR   HOGENOM; CLU_057467_0_1_9; -.
DR   OMA; IAHQKSF; -.
DR   OrthoDB; 1420351at2; -.
DR   Proteomes; UP000000814; Chromosome.
DR   GO; GO:0004096; F:catalase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd01051; Mn_catalase; 1.
DR   Gene3D; 1.20.1260.10; -; 1.
DR   InterPro; IPR012347; Ferritin-like.
DR   InterPro; IPR009078; Ferritin-like_SF.
DR   InterPro; IPR007760; Mn_catalase.
DR   InterPro; IPR039377; Mn_catalase_dom.
DR   Pfam; PF05067; Mn_catalase; 1.
DR   SUPFAM; SSF47240; SSF47240; 1.
PE   3: Inferred from homology;
KW   Calcium; Manganese; Metal-binding; Oxidoreductase; Peroxidase;
KW   Reference proteome.
FT   CHAIN           1..225
FT                   /note="Probable manganese catalase"
FT                   /id="PRO_0000096154"
FT   REGION          204..225
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        209..225
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         37
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         58
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         62
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         67
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         67
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         70
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         138
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         171
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         204
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   225 AA;  25444 MW;  AFE674BBA5344724 CRC64;
     MFKHEKQLLN GMQVKVEREN PQYAVLMQEQ LGGANGELKA AMQYLSQSFR VKDQALKDLF
     LDIGTEELSH MEIVAETINL LNGHSVNYEV VGVGEVESHV LSGLTPFLVN SSGEPWTANY
     VSVTGDIVAD LLSNIASEQR AKVVYEYLYR QINDKEVRRT IDFLLNREEA HNALFREALN
     KVKNEGSNKD FGVTEDSKLY FDLSTPGRYV QDPNPTEPSF SNPRR