MCAPS_TYYVF
ID MCAPS_TYYVF Reviewed; 670 AA.
AC P09514;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Readthrough protein P3-RTD;
DE AltName: Full=P3-P5 readthrough protein {ECO:0000250|UniProtKB:P17525};
DE AltName: Full=P74;
DE AltName: Full=Readthrough protein;
DE Short=RT protein;
DE Contains:
DE RecName: Full=Minor capsid readthrough protein {ECO:0000303|PubMed:20416918};
DE Short=Minor capsid RT protein {ECO:0000303|PubMed:20416918};
DE AltName: Full=54 kDa protein;
DE Contains:
DE RecName: Full=Cleaved product {ECO:0000305};
GN ORFNames=ORF3/ORF5;
OS Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Sobelivirales; Solemoviridae; Polerovirus.
OX NCBI_TaxID=12043;
OH NCBI_TaxID=161934; Beta vulgaris (Sugar beet).
OH NCBI_TaxID=3709; Brassica napus subsp. rapifera.
OH NCBI_TaxID=138011; Brassica napus var. napus.
OH NCBI_TaxID=3710; Brassica nigra (Black mustard) (Sinapis nigra).
OH NCBI_TaxID=3715; Brassica oleracea var. botrytis (Cauliflower).
OH NCBI_TaxID=3716; Brassica oleracea var. capitata (Cabbage).
OH NCBI_TaxID=51350; Brassica rapa subsp. rapa (Turnip).
OH NCBI_TaxID=4072; Capsicum annuum (Capsicum pepper).
OH NCBI_TaxID=3827; Cicer arietinum (Chickpea) (Garbanzo).
OH NCBI_TaxID=3654; Citrullus lanatus (Watermelon) (Citrullus vulgaris).
OH NCBI_TaxID=3721; Crambe hispanica subsp. abyssinica (Abyssinian kale) (Crambe abyssinica).
OH NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH NCBI_TaxID=3663; Cucurbita pepo (Vegetable marrow) (Summer squash).
OH NCBI_TaxID=3847; Glycine max (Soybean) (Glycine hispida).
OH NCBI_TaxID=4232; Helianthus annuus (Common sunflower).
OH NCBI_TaxID=4236; Lactuca sativa (Garden lettuce).
OH NCBI_TaxID=103529; Phlox drummondii (Annual phlox).
OH NCBI_TaxID=3888; Pisum sativum (Garden pea).
OH NCBI_TaxID=3726; Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus).
OH NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH NCBI_TaxID=3562; Spinacia oleracea (Spinach).
OH NCBI_TaxID=3900; Trifolium subterraneum (Subterranean clover).
OH NCBI_TaxID=3906; Vicia faba (Broad bean) (Faba vulgaris).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=3194229; DOI=10.1093/nar/16.21.9917;
RA Veidt I., Lot H., Leiser M., Scheidecker D., Guilley H., Richards K.E.,
RA Jonard G.;
RT "Nucleotide sequence of beet western yellows virus RNA.";
RL Nucleic Acids Res. 16:9917-9932(1988).
RN [2]
RP FUNCTION (MINOR CAPSID READTHROUGH PROTEIN).
RX PubMed=7882968; DOI=10.1002/j.1460-2075.1995.tb07043.x;
RA Brault V., van den Heuvel J.F., Verbeek M., Ziegler-Graff V.,
RA Reutenauer A., Herrbach E., Garaud J.-C., Guilley H., Richards K.E.,
RA Jonard G.;
RT "Aphid transmission of beet western yellows luteovirus requires the minor
RT capsid read-through protein P74.";
RL EMBO J. 14:650-659(1995).
RN [3]
RP DOMAIN (READTHROUGH PROTEIN P3-RTD).
RX PubMed=9143288; DOI=10.1006/viro.1997.8476;
RA Bruyere A., Brault V., Ziegler-Graff V., Simonis M.T., Van den Heuvel J.F.,
RA Richards K., Guilley H., Jonard G., Herrbach E.;
RT "Effects of mutations in the beet western yellows virus readthrough protein
RT on its expression and packaging and on virus accumulation, symptoms, and
RT aphid transmission.";
RL Virology 230:323-334(1997).
RN [4]
RP MUTAGENESIS OF ARG-227; 262-GLU-ASP-263; 316-LYS-ASP-317; 403-LYS-TYR-404
RP AND 428-ASP-GLU-429, AND FUNCTION (MINOR CAPSID READTHROUGH PROTEIN).
RX PubMed=10627524; DOI=10.1128/jvi.74.3.1140-1148.2000;
RA Brault V., Mutterer J., Scheidecker D., Simonis M.T., Herrbach E.,
RA Richards K.E., Ziegler-Graff V.;
RT "Effects of point mutations in the readthrough domain of the beet western
RT yellows virus minor capsid protein on virus accumulation in planta and on
RT transmission by aphids.";
RL J. Virol. 74:1140-1148(2000).
RN [5]
RP ABSENCE OF GLYCOSYLATION (READTHROUGH PROTEIN P3-RTD), PROTEOLYTIC CLEAVAGE
RP (READTHROUGH PROTEIN P3-RTD), AND FUNCTION (MINOR CAPSID READTHROUGH
RP PROTEIN).
RX PubMed=20416918; DOI=10.1016/j.virol.2010.03.037;
RA Revollon S., Strub J.M., Fitchette A.C., Wiss L., Gomord V.,
RA Van Dorsselaer A., Brault V.;
RT "A reinvestigation provides no evidence for sugar residues on structural
RT proteins of poleroviruses and argues against a role for glycosylation of
RT virus structural proteins in aphid transmission.";
RL Virology 402:303-314(2010).
RN [6]
RP FUNCTION (MINOR CAPSID READTHROUGH PROTEIN).
RX PubMed=26402374; DOI=10.1016/j.virol.2015.08.031;
RA Rodriguez-Medina C., Boissinot S., Chapuis S., Gereige D., Rastegar M.,
RA Erdinger M., Revers F., Ziegler-Graff V., Brault V.;
RT "A protein kinase binds the C-terminal domain of the readthrough protein of
RT Turnip yellows virus and regulates virus accumulation.";
RL Virology 486:44-53(2015).
CC -!- FUNCTION: [Minor capsid readthrough protein]: Minor component of the
CC viral capsid involved in aphid transmission and virus accumulation in
CC the host (PubMed:7882968, PubMed:20416918, PubMed:26402374,
CC PubMed:10627524). Required for the virus to move through the aphid (By
CC similarity). The RTD domain of the protein is exposed on the surface of
CC the particle and determines the vector specificity and intestinal
CC tropism in the aphid (PubMed:7882968). {ECO:0000250|UniProtKB:P09516,
CC ECO:0000269|PubMed:10627524, ECO:0000269|PubMed:20416918,
CC ECO:0000269|PubMed:26402374, ECO:0000269|PubMed:7882968}.
CC -!- SUBCELLULAR LOCATION: [Minor capsid readthrough protein]: Virion
CC {ECO:0000250|UniProtKB:P17525}. Host cell junction, host plasmodesma
CC {ECO:0000250|UniProtKB:P17525}. Host periplasm
CC {ECO:0000250|UniProtKB:P17525}.
CC -!- SUBCELLULAR LOCATION: [Cleaved product]: Virion
CC {ECO:0000250|UniProtKB:P17525}.
CC -!- DOMAIN: [Readthrough protein P3-RTD]: The N-terminus is highly basic
CC like those of many plant virus capsid proteins (Probable). These
CC regions may be involved in protein-RNA interaction (Probable). The RTD
CC N-terminus is responsible for aphid transmission and aphid endosymbiont
CC interaction (PubMed:9143288). The readthrough domain is required for
CC transport of virus through membranes of the aphid salivary glands (By
CC similarity). {ECO:0000250|UniProtKB:P09516, ECO:0000269|PubMed:9143288,
CC ECO:0000305}.
CC -!- PTM: [Readthrough protein P3-RTD]: In virus particles, more than 200
CC amino acids are proteolytically cleaved releasing the C-terminus part
CC of the RTD domain (PubMed:20416918). The cleaved product remains
CC attached to the virus particle (By similarity).
CC {ECO:0000250|UniProtKB:P17525, ECO:0000269|PubMed:20416918}.
CC -!- MISCELLANEOUS: [Readthrough protein P3-RTD]: This protein is translated
CC as a fusion protein by episodic readthrough of the major coat protein
CC termination codon. It is composed of the major capsid protein fused to
CC a long C-terminal extension called the readthrough domain (RTD).
CC Readthrough of the terminator codon TAG occurs between the codons for
CC 202-Lys and 204-Val. {ECO:0000250|UniProtKB:P17525}.
CC -!- SIMILARITY: Belongs to the luteoviruses readthrough protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA31467.2; Type=Miscellaneous discrepancy; Note=Readthrough of an in-frame TAG stop codon in position 203 translated as Tyr.; Evidence={ECO:0000305};
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DR EMBL; X13063; CAA31467.2; ALT_SEQ; Genomic_RNA.
DR PIR; S01943; S01943.
DR RefSeq; NP_620487.1; NC_003743.1.
DR SMR; P09514; -.
DR GeneID; 940480; -.
DR KEGG; vg:940480; -.
DR Proteomes; UP000007545; Genome.
DR GO; GO:0044229; C:host cell periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR InterPro; IPR001517; Luteo_coat.
DR InterPro; IPR002929; PLrV_ORF5.
DR Pfam; PF00894; Luteo_coat; 1.
DR Pfam; PF01690; PLRV_ORF5; 1.
PE 1: Evidence at protein level;
KW Capsid protein; Host cell junction; Host periplasm; Reference proteome;
KW RNA suppression of termination; Virion.
FT CHAIN 1..670
FT /note="Readthrough protein P3-RTD"
FT /id="PRO_0000222426"
FT CHAIN 1..437
FT /note="Minor capsid readthrough protein"
FT /id="PRO_0000455349"
FT CHAIN 438..670
FT /note="Cleaved product"
FT /id="PRO_0000455350"
FT REGION 1..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 203..223
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 204..670
FT /note="Readthrough domain (RTD)"
FT REGION 431..553
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 609..629
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 641..670
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..52
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 207..221
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 464..484
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 512..528
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 437..438
FT /note="Cleavage"
FT /evidence="ECO:0000269|PubMed:20416918"
FT MUTAGEN 227
FT /note="R->A: Complete loss of aphid transmission."
FT /evidence="ECO:0000269|PubMed:10627524"
FT MUTAGEN 262..263
FT /note="ED->A: Complete loss of aphid transmission."
FT /evidence="ECO:0000269|PubMed:10627524"
FT MUTAGEN 316..317
FT /note="KD->A: No effect on aphid transmission."
FT /evidence="ECO:0000269|PubMed:10627524"
FT MUTAGEN 403..404
FT /note="KY->A: Complete loss of aphid transmission."
FT /evidence="ECO:0000269|PubMed:10627524"
FT MUTAGEN 428..429
FT /note="DE->A: Almost complete loss of aphid transmission."
FT /evidence="ECO:0000269|PubMed:10627524"
SQ SEQUENCE 670 AA; 74016 MW; 4301B21EE7B4BE9F CRC64;
MNTVVGRRII NGRRRPRRQT RRAQRPQPVV VVQTSRATQR RPRRRRRGNN RTGRTVPTRG
AGSSETFVFS KDNLAGSSSG AITFGPSLSD CPAFSNGMLK AYHEYKISMV ILEFVSEASS
QNSGSIAYEL DPHCKLNSLS STINKFGITK PGKRAFTASY INGTEWHDVA EDQFRILYKG
NGSSSIAGSF RITIKCQFHN PKYVDEEPGP SPGPSPSPQP TPQKKYRFIV YTGVPVTRIM
AQSTDDAISL YDMPSQRFRY IEDENMNWTN LDSRWYSQNS LKAIPMIIVP VPQGEWTVEI
SMEGYQPTSS TTDPNKDKQD GLIAYNDDLS EGWNVGIYNN VEITNNKADN TLKYGHPDME
LNGCHFNQGQ CLERDGDLTC HIKTTGDNAS FFVVGPAVQK QSKYNYAVSY GAWTDRMMEI
GMIAIALDEQ GSSGSVKTER PKRVGHSMAV STWETIKLPE KGNSEGYETS QRQDSKTPPT
ASGGSDTLDV EEGGLPLPVE EEIPDFVGDN PWSDLSTKNS QEEEAMSSES GLRPQLKPPG
LPKPQPIRTI RNFDPTPDLV EAWRPDVNPG YSKADVAAAT IIAGGSIKDG RSMIDKRNKA
VLDGRKSWGS SLASSLTGGT LKASAKSEKL AKLTTSERAR YERIKRQQGS TRASEFLESL
LAGEDPDSRF
