MCAPS_PLRVW
ID MCAPS_PLRVW Reviewed; 716 AA.
AC P11626;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=Readthrough protein P3-RTD;
DE AltName: Full=P3-P5 readthrough protein;
DE AltName: Full=P74;
DE AltName: Full=Readthrough protein;
DE Short=RT protein;
DE Contains:
DE RecName: Full=Minor capsid readthrough protein {ECO:0000250|UniProtKB:P09514};
DE Short=Minor capsid RT protein {ECO:0000250|UniProtKB:P09514};
DE AltName: Full=54 kDa protein;
DE Contains:
DE RecName: Full=Cleaved product {ECO:0000305};
GN ORFNames=ORF3/ORF5;
OS Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Sobelivirales; Solemoviridae; Polerovirus.
OX NCBI_TaxID=12048;
OH NCBI_TaxID=4113; Solanum tuberosum (Potato).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2466700; DOI=10.1016/0014-5793(89)80190-5;
RA van der Wilk F., Huisman M.J., Cornelissen B.J.C., Huttinga H.,
RA Goldbach R.W.;
RT "Nucleotide sequence and organization of potato leafroll virus genomic
RT RNA.";
RL FEBS Lett. 245:51-56(1989).
CC -!- FUNCTION: [Minor capsid readthrough protein]: Minor component of the
CC viral capsid involved in aphid transmission and virus accumulation in
CC the host (By similarity). Required for the virus to move through the
CC aphid (By similarity). The RTD domain of the protein is exposed on the
CC surface of the particle and determines the vector specificity and
CC intestinal tropism in the aphid (By similarity). This domain might also
CC determine the limitation of the virus to the host phloem (By
CC similarity). {ECO:0000250|UniProtKB:P09514,
CC ECO:0000250|UniProtKB:P09516, ECO:0000250|UniProtKB:P17525}.
CC -!- FUNCTION: [Isoform P7]: Participates, together with the proteins P0 and
CC P1, in the inhibition of the induction of aphid-induced host
CC phytohormones. This could play a role in the attraction to the infected
CC plants by aphids. {ECO:0000250|UniProtKB:P17525}.
CC -!- SUBCELLULAR LOCATION: [Minor capsid readthrough protein]: Virion
CC {ECO:0000250|UniProtKB:P17525}. Host cell junction, host plasmodesma
CC {ECO:0000250|UniProtKB:P17525}. Host periplasm
CC {ECO:0000250|UniProtKB:P17525}.
CC -!- SUBCELLULAR LOCATION: [Cleaved product]: Virion
CC {ECO:0000250|UniProtKB:P17525}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative promoter usage; Named isoforms=2;
CC Name=Readthrough protein P3-RTD;
CC IsoId=P11626-1; Sequence=Displayed;
CC Name=P7;
CC IsoId=P11626-2; Sequence=VSP_038674;
CC -!- DOMAIN: [Isoform Readthrough protein P3-RTD]: The N-terminus is highly
CC basic like those of many plant virus capsid proteins. These regions may
CC be involved in protein-RNA interaction (By similarity). The RTD N-
CC terminus is responsible for aphid transmission and aphid endosymbiont
CC interaction (By similarity). The readthrough domain is required for
CC transport of virus through membranes of the aphid salivary glands (By
CC similarity). {ECO:0000250|UniProtKB:P09514,
CC ECO:0000250|UniProtKB:P09516, ECO:0000250|UniProtKB:P17525}.
CC -!- PTM: [Isoform Readthrough protein P3-RTD]: In virus particles, more
CC than 200 amino acids are proteolytically cleaved releasing the C-
CC terminus part of the RTD domain. The cleaved product remains attached
CC to the virus particle. {ECO:0000250|UniProtKB:P17525}.
CC -!- MISCELLANEOUS: [Isoform Readthrough protein P3-RTD]: This protein is
CC translated as a fusion protein by episodic readthrough of the major
CC coat protein termination codon. It is composed of the major capsid
CC protein fused to a long C-terminal extension called the readthrough
CC domain (RTD). Readthrough of the terminator codon TAG occurs between
CC the codons for 208-Lys and 210-Val. {ECO:0000250|UniProtKB:P17525}.
CC -!- SIMILARITY: Belongs to the luteoviruses readthrough protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA68799.1; Type=Miscellaneous discrepancy; Note=Readthrough of an in-frame TAG stop codon in position 209 translated as Tyr.; Evidence={ECO:0000305};
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DR EMBL; Y07496; CAA68799.1; ALT_SEQ; Genomic_RNA.
DR PIR; S03551; S03551.
DR SMR; P11626; -.
DR Proteomes; UP000000474; Genome.
DR GO; GO:0044229; C:host cell periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR Gene3D; 2.60.120.20; -; 1.
DR InterPro; IPR001517; Luteo_coat.
DR InterPro; IPR002929; PLrV_ORF5.
DR InterPro; IPR029053; Viral_coat.
DR Pfam; PF00894; Luteo_coat; 1.
DR Pfam; PF01690; PLRV_ORF5; 1.
PE 3: Inferred from homology;
KW Alternative promoter usage; Capsid protein; Host cell junction;
KW Host periplasm; RNA suppression of termination; Virion.
FT CHAIN 1..716
FT /note="Readthrough protein P3-RTD"
FT /id="PRO_0000222430"
FT CHAIN 1..?443
FT /note="Minor capsid readthrough protein"
FT /id="PRO_0000455347"
FT CHAIN ?444..716
FT /note="Cleaved product"
FT /id="PRO_0000455348"
FT REGION 1..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 207..228
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 210..716
FT /note="Readthrough domain (RTD)"
FT REGION 438..547
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 694..698
FT /note="Involved in the efficient long distance movement of
FT the virus and the periplasmic subcellular location"
FT /evidence="ECO:0000250|UniProtKB:P17525"
FT COMPBIAS 43..62
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 212..226
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..457
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 464..478
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 479..521
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 443..444
FT /note="Cleavage"
FT /evidence="ECO:0000250|UniProtKB:P09514"
FT VAR_SEQ 1..591
FT /note="Missing (in isoform P7)"
FT /evidence="ECO:0000305"
FT /id="VSP_038674"
SQ SEQUENCE 716 AA; 79759 MW; BBD2F9374542CAB0 CRC64;
MSTVVVKGNV NGGVQQPRRR RRQSLRRRAN RVQPVVMVTA PGQPRRRRRR RGGNRRSRRT
GVPRGRGSSE TFVFTKDNLM GNSQGSFTFG PSLSDCPAFK DGILKAYHEY KITSILLQFV
SEASSTSSGS IAYELDPHCK VSSLQSYVNQ FQIPQGGAKT YQARMINGVE WHDSSEDQCR
ILWKGNGKSS DTAGSFRVTI RVALQNPKYD SGPEPGPSPQ PTPTPTPQKH ERFIAYVGIP
MLTIQARESD DQIILGSLGS QRMKYIEDEN QNYTNVSSEY YSQSSMQAVP MYYFNVPKGQ
WSVDISCEGY QPTSSTSDPN RGRSDGMIAY SNADSDYWNV GEADGVKISK LRNDNTYRQG
HPELEINSCH FREGQLLERD ATISFHVEAP TDGRFFLVGP AIQKTAKYNY TISYGDWTDR
DMELGLITVV LDEHLEGTGS ANRVRRPPRE GHTYMASPRE PEGKPVGNKP RDETPIQTQE
RQPDQTPSDD VSDAGSVNSG GSTESLQLEF GANSDSTYDA TVDGTDWPRI PPPRHPPEPR
VSGNSRTVTD FSPKADLLEN WDAKHFDPGY SKEDVAAATI IAHGSIQDGR SMLEKREENV
KNKTSSWKPP LPKAVSPAIA KLRSIRKSQP LEGGTLKKDA TDGVSSIGSG SLTGGTLKRK
ETIEERLLQT LTTEQRLWYE NLKKTNPLAA TQWLFEYQPP PQVDRNLAEK PFQGRK
