MCAPS_BYDVP
ID MCAPS_BYDVP Reviewed; 651 AA.
AC P09516;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 25-MAY-2022, sequence version 4.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Readthrough protein P3-RTD;
DE AltName: Full=P3-P5 readthrough protein {ECO:0000250|UniProtKB:P17525};
DE AltName: Full=P72 {ECO:0000303|PubMed:7975225};
DE AltName: Full=Readthrough protein;
DE Short=RT protein;
DE Contains:
DE RecName: Full=Minor capsid readthrough protein {ECO:0000250|UniProtKB:P09514};
DE Short=Minor capsid RT protein {ECO:0000250|UniProtKB:P09514};
DE AltName: Full=P50 {ECO:0000303|PubMed:7975225};
DE AltName: Full=P58 {ECO:0000303|PubMed:7856106};
DE Contains:
DE RecName: Full=Cleaved product {ECO:0000305};
DE AltName: Full=P33 {ECO:0000303|PubMed:7975225};
GN ORFNames=ORF3/ORF5;
OS Barley yellow dwarf virus (isolate PAV) (BYDV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC Tolivirales; Tombusviridae; Luteovirus.
OX NCBI_TaxID=2169986;
OH NCBI_TaxID=146531; Avena byzantina.
OH NCBI_TaxID=4498; Avena sativa (Oat).
OH NCBI_TaxID=4513; Hordeum vulgare (Barley).
OH NCBI_TaxID=4521; Lolium multiflorum (Italian ryegrass) (Lolium perenne subsp. multiflorum).
OH NCBI_TaxID=4522; Lolium perenne (Perennial ryegrass).
OH NCBI_TaxID=4530; Oryza sativa (Rice).
OH NCBI_TaxID=4550; Secale cereale (Rye).
OH NCBI_TaxID=4565; Triticum aestivum (Wheat).
OH NCBI_TaxID=4577; Zea mays (Maize).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=3399386; DOI=10.1093/nar/16.13.6097;
RA Miller W.A., Waterhouse P.M., Gerlach W.L.;
RT "Sequence and organization of barley yellow dwarf virus genomic RNA.";
RL Nucleic Acids Res. 16:6097-6111(1988).
RN [2]
RP READTHROUGH (READTHROUGH PROTEIN P3-RTD).
RX PubMed=8030200; DOI=10.1006/viro.1994.1427;
RA Cheng S.L., Domier L.L., D'Arcy C.J.;
RT "Detection of the readthrough protein of barley yellow dwarf virus.";
RL Virology 202:1003-1006(1994).
RN [3]
RP PROTEOLYTIC CLEAVAGE (READTHROUGH PROTEIN P3-RTD), SUBCELLULAR LOCATION
RP (CLEAVED PRODUCT), AND SUBCELLULAR LOCATION (MINOR CAPSID READTHROUGH
RP PROTEIN).
RX PubMed=7975225; DOI=10.1006/viro.1994.1645;
RA Filichkin S.A., Lister R.M., McGrath P.F., Young M.J.;
RT "In vivo expression and mutational analysis of the barley yellow dwarf
RT virus readthrough gene.";
RL Virology 205:290-299(1994).
RN [4]
RP SUBCELLULAR LOCATION (MINOR CAPSID READTHROUGH PROTEIN).
RX PubMed=7856106; DOI=10.1006/viro.1995.1018;
RA Wang J.Y., Chay C., Gildow F.E., Gray S.M.;
RT "Readthrough protein associated with virions of barley yellow dwarf
RT luteovirus and its potential role in regulating the efficiency of aphid
RT transmission.";
RL Virology 206:954-962(1995).
RN [5]
RP FUNCTION (MINOR CAPSID READTHROUGH PROTEIN), AND DOMAIN (READTHROUGH
RP PROTEIN P3-RTD).
RX PubMed=8623554; DOI=10.1006/viro.1996.0222;
RA Chay C.A., Gunasinge U.B., Dinesh-Kumar S.P., Miller W.A., Gray S.M.;
RT "Aphid transmission and systemic plant infection determinants of barley
RT yellow dwarf luteovirus-PAV are contained in the coat protein readthrough
RT domain and 17-kDa protein, respectively.";
RL Virology 219:57-65(1996).
CC -!- FUNCTION: [Minor capsid readthrough protein]: Minor component of the
CC viral capsid involved in aphid transmission and virus accumulation in
CC the host (PubMed:8623554). Required fro the virus to move through the
CC aphid (PubMed:8623554). The RTD domain of the protein is exposed on the
CC surface of the particle and determines the vector specificity and
CC intestinal tropism in the aphid (By similarity).
CC {ECO:0000250|UniProtKB:P09514, ECO:0000269|PubMed:8623554}.
CC -!- SUBCELLULAR LOCATION: [Cleaved product]: Membrane
CC {ECO:0000269|PubMed:7975225}.
CC -!- SUBCELLULAR LOCATION: [Minor capsid readthrough protein]: Virion
CC {ECO:0000269|PubMed:7856106, ECO:0000269|PubMed:7975225}. Host cell
CC junction, host plasmodesma {ECO:0000250|UniProtKB:P17525}. Host
CC periplasm {ECO:0000250|UniProtKB:P17525}.
CC -!- DOMAIN: [Readthrough protein P3-RTD]: The N-terminus is highly basic
CC like those of many plant virus capsid proteins (Probable). These
CC regions may be involved in protein-RNA interaction (Probable). The RTD
CC N-terminus is responsible for aphid transmission and aphid endosymbiont
CC interaction (By similarity). The readthrough domain is required for
CC transport of virus through membranes of the aphid salivary glands
CC (PubMed:8623554). {ECO:0000250|UniProtKB:P09514,
CC ECO:0000269|PubMed:8623554, ECO:0000305}.
CC -!- PTM: [Readthrough protein P3-RTD]: In virus particles, more than 200
CC amino acids are proteolytically cleaved releasing the C-terminus part
CC of the RTD domain (Probable). The cleaved product remains attached to
CC the virus particle (By similarity). {ECO:0000250|UniProtKB:P17525,
CC ECO:0000305|PubMed:7975225}.
CC -!- MISCELLANEOUS: This protein is translated as a fusion protein by
CC episodic readthrough of the major coat protein termination codon. It is
CC composed of the major capsid protein fused to a long C-terminal
CC extension called the readthrough domain (RTD). Readthrough of the
CC terminator codon TAG occurs between the codons for 200-Lys and 202-Val.
CC {ECO:0000305|PubMed:8030200}.
CC -!- SIMILARITY: Belongs to the luteoviruses readthrough protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA30495.2; Type=Miscellaneous discrepancy; Note=Readthrough of an in-frame TAG stop codon in position 201 translated as Tyr.; Evidence={ECO:0000305};
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DR EMBL; X07653; CAA30495.2; ALT_SEQ; Genomic_RNA.
DR PIR; S00950; S00950.
DR SMR; P09516; -.
DR Proteomes; UP000006722; Genome.
DR GO; GO:0044229; C:host cell periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR InterPro; IPR001517; Luteo_coat.
DR InterPro; IPR002929; PLrV_ORF5.
DR Pfam; PF00894; Luteo_coat; 1.
DR Pfam; PF01690; PLRV_ORF5; 2.
DR PRINTS; PR00915; LUTEOGP1COAT.
PE 1: Evidence at protein level;
KW Capsid protein; Host cell junction; Host periplasm; Membrane;
KW Reference proteome; RNA suppression of termination; Virion.
FT CHAIN 1..651
FT /note="Readthrough protein P3-RTD"
FT /id="PRO_0000222428"
FT CHAIN 1..460
FT /note="Minor capsid readthrough protein"
FT /id="PRO_0000455343"
FT CHAIN 461..651
FT /note="Cleaved product"
FT /id="PRO_0000455344"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 33..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 201..241
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 202..650
FT /note="Readthrough domain (RTD)"
FT REGION 532..552
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 606..626
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 208..236
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 651 AA; 71874 MW; 8645B2DA52B8B253 CRC64;
MNSVGRRGPR RANQNGTRRR RRRTVRPVVV VQPNRAGPRR RNGRRKGRGG ANFVFRPTGG
TEVFVFSVDN LKANSSGAIK FGPSLSQCPA LSDGILKSYH RYKITSIRVE FKSHASANTA
GAIFIELDTA CKQSALGSYI NSFTISKTAS KTFRSEAING KEFQESTIDQ FWMLYKANGT
TTDTAGQFII TMSVSLMTAK XVDSSTPEPK PAPEPTPTPQ PTPAPQPTPE PTPAPVPKRF
FEYIGTPTGT ISTRENTDSI SVSKLGGQSM QYIENEKCET KVIDSFWSTN NNVSAQAAFV
YPVPEGSYSV NISCEGFQSV DHIGGNEDGY WIGLIAYSNS SGDNWGVGNY KGCSFKNFLA
TNTWRPGHKD LKLTDCQFTD GQIVERDAVM SFHVEATGKD ASFYLMAPKT MKTDKYNYVV
SYGGYTNKRM EFGTISVTCD ESDVEAERIT RHAETPIRSK HILVSERYAE PLPTIVNQGL
CDVKTPEQEQ TLVDEDDRQT VSTESDIALL EYEAATAEIP DAEEDVLPSK EQLSSKPMDT
SGNIIPKPKE PEVLGTYQGQ NIYPEDVPPM ARQKLREAAN APSTLLYERR TPKKSGNFLS
RLVEANRSPT TPTAPSVSTT SNMTREQLRE YTRIRNSSGI TAAKAYKAQF Q
