MCAPS_BWYVG
ID MCAPS_BWYVG Reviewed; 673 AA.
AC P09515;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Readthrough protein P3-RTD;
DE AltName: Full=P3-P5 readthrough protein {ECO:0000250|UniProtKB:P17525};
DE AltName: Full=P74;
DE AltName: Full=Readthrough protein;
DE Short=RT protein;
DE Contains:
DE RecName: Full=Minor capsid readthrough protein {ECO:0000250|UniProtKB:P09514};
DE Short=Minor capsid RT protein {ECO:0000250|UniProtKB:P09514};
DE Contains:
DE RecName: Full=Cleaved product {ECO:0000305};
DE Flags: Fragment;
GN ORFNames=ORF3/ORF5;
OS Beet western yellows virus (isolate GB1) (BWYV).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Sobelivirales; Solemoviridae; Polerovirus.
OX NCBI_TaxID=12044;
OH NCBI_TaxID=161934; Beta vulgaris (Sugar beet).
OH NCBI_TaxID=3709; Brassica napus subsp. rapifera.
OH NCBI_TaxID=138011; Brassica napus var. napus.
OH NCBI_TaxID=3710; Brassica nigra (Black mustard) (Sinapis nigra).
OH NCBI_TaxID=3715; Brassica oleracea var. botrytis (Cauliflower).
OH NCBI_TaxID=3716; Brassica oleracea var. capitata (Cabbage).
OH NCBI_TaxID=51350; Brassica rapa subsp. rapa (Turnip).
OH NCBI_TaxID=4072; Capsicum annuum (Capsicum pepper).
OH NCBI_TaxID=3827; Cicer arietinum (Chickpea) (Garbanzo).
OH NCBI_TaxID=3654; Citrullus lanatus (Watermelon) (Citrullus vulgaris).
OH NCBI_TaxID=3721; Crambe hispanica subsp. abyssinica (Abyssinian kale) (Crambe abyssinica).
OH NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH NCBI_TaxID=3663; Cucurbita pepo (Vegetable marrow) (Summer squash).
OH NCBI_TaxID=3847; Glycine max (Soybean) (Glycine hispida).
OH NCBI_TaxID=4232; Helianthus annuus (Common sunflower).
OH NCBI_TaxID=4236; Lactuca sativa (Garden lettuce).
OH NCBI_TaxID=103529; Phlox drummondii (Annual phlox).
OH NCBI_TaxID=3888; Pisum sativum (Garden pea).
OH NCBI_TaxID=3726; Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus).
OH NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH NCBI_TaxID=3562; Spinacia oleracea (Spinach).
OH NCBI_TaxID=3900; Trifolium subterraneum (Subterranean clover).
OH NCBI_TaxID=3906; Vicia faba (Broad bean) (Faba vulgaris).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=3194229; DOI=10.1093/nar/16.21.9917;
RA Veidt I., Lot H., Leiser M., Scheidecker D., Guilley H., Richards K.E.,
RA Jonard G.;
RT "Nucleotide sequence of beet western yellows virus RNA.";
RL Nucleic Acids Res. 16:9917-9932(1988).
RN [2]
RP FUNCTION (MINOR CAPSID READTHROUGH PROTEIN).
RX PubMed=16014930; DOI=10.1128/jvi.79.15.9685-9693.2005;
RA Brault V., Perigon S., Reinbold C., Erdinger M., Scheidecker D.,
RA Herrbach E., Richards K.E., Ziegler-Graff V.;
RT "The polerovirus minor capsid protein determines vector specificity and
RT intestinal tropism in the aphid.";
RL J. Virol. 79:9685-9693(2005).
CC -!- FUNCTION: [Minor capsid readthrough protein]: Minor component of the
CC viral capsid involved in aphid transmission and virus accumulation in
CC the host (PubMed:16014930). Required for the virus to move through the
CC aphid (By similarity). The RTD domain of the protein is exposed on the
CC surface of the particle and determines the vector specificity and
CC intestinal tropism in the aphid (PubMed:16014930).
CC {ECO:0000250|UniProtKB:P09516, ECO:0000269|PubMed:16014930}.
CC -!- SUBCELLULAR LOCATION: [Minor capsid readthrough protein]: Virion
CC {ECO:0000250|UniProtKB:P17525}. Host cell junction, host plasmodesma
CC {ECO:0000250|UniProtKB:P17525}. Host periplasm
CC {ECO:0000250|UniProtKB:P17525}.
CC -!- SUBCELLULAR LOCATION: [Cleaved product]: Virion
CC {ECO:0000250|UniProtKB:P17525}.
CC -!- DOMAIN: [Readthrough protein P3-RTD]: The N-terminus is highly basic
CC like those of many plant virus capsid proteins (Probable). These
CC regions may be involved in protein-RNA interaction (Probable). The RTD
CC N-terminus is responsible for aphid transmission and aphid endosymbiont
CC interaction (By similarity). The readthrough domain is required for
CC transport of virus through membranes of the aphid salivary glands (By
CC similarity). {ECO:0000250|UniProtKB:P09514,
CC ECO:0000250|UniProtKB:P09516, ECO:0000305}.
CC -!- PTM: [Readthrough protein P3-RTD]: In virus particles, more than 200
CC amino acids are proteolytically cleaved releasing the C-terminus part
CC of the RTD domain (By similarity). The cleaved product remains attached
CC to the virus particle (By similarity). {ECO:0000250|UniProtKB:P09514,
CC ECO:0000250|UniProtKB:P17525}.
CC -!- MISCELLANEOUS: [Readthrough protein P3-RTD]: This protein is translated
CC as a fusion protein by episodic readthrough of the major coat protein
CC termination codon. It is composed of the major capsid protein fused to
CC a long C-terminal extension called the readthrough domain (RTD).
CC Readthrough of the terminator codon TAG occurs between the codons for
CC 201-Lys and 203-Val (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the luteoviruses readthrough protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA31461.1; Type=Miscellaneous discrepancy; Note=Readthrough of an in-frame TAG stop codon in position 202 translated as Tyr.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X13062; CAA31461.1; ALT_SEQ; Genomic_RNA.
DR PIR; S01937; S01937.
DR SMR; P09515; -.
DR GO; GO:0044229; C:host cell periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR InterPro; IPR001517; Luteo_coat.
DR InterPro; IPR002929; PLrV_ORF5.
DR Pfam; PF00894; Luteo_coat; 1.
DR Pfam; PF01690; PLRV_ORF5; 1.
PE 3: Inferred from homology;
KW Capsid protein; Host cell junction; Host periplasm; Virion.
FT CHAIN <1..673
FT /note="Readthrough protein P3-RTD"
FT /id="PRO_0000222427"
FT CHAIN 1..436
FT /note="Minor capsid readthrough protein"
FT /id="PRO_0000455341"
FT CHAIN 437..673
FT /note="Cleaved product"
FT /id="PRO_0000455342"
FT REGION 1..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 202..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 203..673
FT /note="Readthrough domain (RTD)"
FT REGION 203..669
FT /note="Readthrough domain (RTD)"
FT /evidence="ECO:0000250|UniProtKB:P09514"
FT REGION 456..490
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 503..547
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..36
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 37..51
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 206..220
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 509..533
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 436..437
FT /note="Cleavage"
FT /evidence="ECO:0000250|UniProtKB:P09514"
FT NON_TER 1
SQ SEQUENCE 673 AA; 74593 MW; 906C622AF64ACC37 CRC64;
NTVVGRRTIN GRRRPRRQTR RAQRSQPVVV VQTSRATQRR PRRRRRGNNR TRGTVPTRGA
GSSETFVFSK DNLAGSSSGR ITFGPSLSDC PAFSNGILKA YHEYKISMVI LEFVSEASSQ
NSGSIAYELD PHCKLNSLSS TINKFGITKP GKAAFTASYI NGKEWHDVAE DQFRILYKGN
GSSSIAGSFR ITIKCQFHNP KYVDEEPGPS PGPSPSPQPT PQKKYRFIVY TGVPVTRIMA
QSTDDAISLY DMPSQRFRYI EDENMNWTNL DSRWYSQNSL KAIPMIIVPV PQGEWTVEIS
MEGYQPTSST TDPNKDKQDG LIAYNDDLSE GWNVGIYNNV EITNNKADNT LKYGHPDMEL
NGCHFNQGQC LERDGDLTCH IKTTGDNASF FVGRPAVQKQ SKYNYAVSYG AWTDRMMEIG
MIAIALDEQG SSGSAKTKRP KRVGHSMAVS TWETINLPEK ENSEEIQTSQ RQDFKTPPTA
GGGSDMLDVE EGGLPLSVEE EIPDFVGDNP WSNITTENSQ EEEAMSSKSG LTPQLKPPGL
PKPQPIRRLK SFDATPDLVE AWRPDVNPGY SKADWAVATI IAGGSIKDGR SMIDKRDKAV
LDGRKSWGSS LASSLTGGTL KASAKSEKLA KLTTSERARF EQIKRQQGSV RASEFLEQIL
AGKDPDPKFL RDR
