MCAC2_ARATH
ID MCAC2_ARATH Reviewed; 416 AA.
AC Q3EBY6; A2PYH1; Q2V480; Q2V481; Q2V482;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Protein MID1-COMPLEMENTING ACTIVITY 2;
GN Name=MCA2; OrderedLocusNames=At2g17780; ORFNames=T17A5.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=17360695; DOI=10.1073/pnas.0607703104;
RA Nakagawa Y., Katagiri T., Shinozaki K., Qi Z., Tatsumi H., Furuichi T.,
RA Kishigami A., Sokabe M., Kojima I., Sato S., Kato T., Tabata S., Iida K.,
RA Terashima A., Nakano M., Ikeda M., Yamanaka T., Iida H.;
RT "Arabidopsis plasma membrane protein crucial for Ca2+ influx and touch
RT sensing in roots.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:3639-3644(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ACTIVITY REGULATION,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=20097794; DOI=10.1104/pp.109.147371;
RA Yamanaka T., Nakagawa Y., Mori K., Nakano M., Imamura T., Kataoka H.,
RA Terashima A., Iida K., Kojima I., Katagiri T., Shinozaki K., Iida H.;
RT "MCA1 and MCA2 that mediate Ca2+ uptake have distinct and overlapping roles
RT in Arabidopsis.";
RL Plant Physiol. 152:1284-1296(2010).
CC -!- FUNCTION: Calcium-permeable stretch-activated channel component.
CC Probably involved in mechanosensing and in mechano-stimulated calcium
CC uptake mechanism. {ECO:0000269|PubMed:20097794}.
CC -!- ACTIVITY REGULATION: Inhibited by GdCl(3), but not by verapamil.
CC {ECO:0000269|PubMed:20097794}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20097794};
CC Single-pass membrane protein {ECO:0000269|PubMed:20097794}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q3EBY6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3EBY6-2; Sequence=VSP_040966;
CC Name=3;
CC IsoId=Q3EBY6-3; Sequence=VSP_040967;
CC Name=4;
CC IsoId=Q3EBY6-4; Sequence=VSP_040965;
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems, flowers and
CC siliques. In the root, high levels of expression in vascular tissues,
CC in the stele and endodermis, but no expression in the cortex,
CC epidermis, root cap, promeristem and adjacent elongation zone of the
CC primary root. Not expressed in root hairs. Detected in shoot apical
CC meristem, leaf mesophyll cells and vascular tissues, upper half of
CC inflorescence, but not in petioles of rosette leaves.
CC {ECO:0000269|PubMed:20097794}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype when grown under normal
CC conditions, due to redundancy with MCA1. The roots are able to normally
CC sense the hardness of the growth medium. Mca1 and mca2 double mutant
CC shows a strong growth defect. {ECO:0000269|PubMed:20097794}.
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DR EMBL; AB196961; BAF46390.1; -; mRNA.
DR EMBL; CP002685; AEC06682.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06683.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06684.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06685.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62012.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62013.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62015.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62016.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62017.1; -; Genomic_DNA.
DR RefSeq; NP_001031366.1; NM_001036289.1. [Q3EBY6-2]
DR RefSeq; NP_001031367.1; NM_001036290.2. [Q3EBY6-3]
DR RefSeq; NP_001031368.1; NM_001036291.1. [Q3EBY6-4]
DR RefSeq; NP_001324195.1; NM_001335561.1. [Q3EBY6-3]
DR RefSeq; NP_001324196.1; NM_001335556.1. [Q3EBY6-1]
DR RefSeq; NP_001324198.1; NM_001335557.1. [Q3EBY6-1]
DR RefSeq; NP_001324199.1; NM_001335558.1. [Q3EBY6-1]
DR RefSeq; NP_001324200.1; NM_001335559.1. [Q3EBY6-1]
DR RefSeq; NP_179369.2; NM_127332.3. [Q3EBY6-1]
DR AlphaFoldDB; Q3EBY6; -.
DR SMR; Q3EBY6; -.
DR STRING; 3702.AT2G17780.1; -.
DR TCDB; 1.A.87.1.2; the mechanosensitive calcium channel (mca) family.
DR PaxDb; Q3EBY6; -.
DR PRIDE; Q3EBY6; -.
DR ProteomicsDB; 238890; -. [Q3EBY6-1]
DR EnsemblPlants; AT2G17780.1; AT2G17780.1; AT2G17780. [Q3EBY6-1]
DR EnsemblPlants; AT2G17780.10; AT2G17780.10; AT2G17780. [Q3EBY6-1]
DR EnsemblPlants; AT2G17780.2; AT2G17780.2; AT2G17780. [Q3EBY6-2]
DR EnsemblPlants; AT2G17780.3; AT2G17780.3; AT2G17780. [Q3EBY6-3]
DR EnsemblPlants; AT2G17780.4; AT2G17780.4; AT2G17780. [Q3EBY6-4]
DR EnsemblPlants; AT2G17780.5; AT2G17780.5; AT2G17780. [Q3EBY6-1]
DR EnsemblPlants; AT2G17780.6; AT2G17780.6; AT2G17780. [Q3EBY6-1]
DR EnsemblPlants; AT2G17780.7; AT2G17780.7; AT2G17780. [Q3EBY6-1]
DR EnsemblPlants; AT2G17780.9; AT2G17780.9; AT2G17780. [Q3EBY6-3]
DR GeneID; 816287; -.
DR Gramene; AT2G17780.1; AT2G17780.1; AT2G17780. [Q3EBY6-1]
DR Gramene; AT2G17780.10; AT2G17780.10; AT2G17780. [Q3EBY6-1]
DR Gramene; AT2G17780.2; AT2G17780.2; AT2G17780. [Q3EBY6-2]
DR Gramene; AT2G17780.3; AT2G17780.3; AT2G17780. [Q3EBY6-3]
DR Gramene; AT2G17780.4; AT2G17780.4; AT2G17780. [Q3EBY6-4]
DR Gramene; AT2G17780.5; AT2G17780.5; AT2G17780. [Q3EBY6-1]
DR Gramene; AT2G17780.6; AT2G17780.6; AT2G17780. [Q3EBY6-1]
DR Gramene; AT2G17780.7; AT2G17780.7; AT2G17780. [Q3EBY6-1]
DR Gramene; AT2G17780.9; AT2G17780.9; AT2G17780. [Q3EBY6-3]
DR KEGG; ath:AT2G17780; -.
DR Araport; AT2G17780; -.
DR TAIR; locus:2827953; AT2G17780.
DR eggNOG; ENOG502QQIG; Eukaryota.
DR InParanoid; Q3EBY6; -.
DR OMA; HENWHAD; -.
DR OrthoDB; 1354725at2759; -.
DR PhylomeDB; Q3EBY6; -.
DR PRO; PR:Q3EBY6; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q3EBY6; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0005262; F:calcium channel activity; IDA:TAIR.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR GO; GO:0048528; P:post-embryonic root development; IGI:TAIR.
DR Gene3D; 1.20.930.20; -; 1.
DR InterPro; IPR036537; Adaptor_Cbl_N_dom_sf.
DR InterPro; IPR045766; MCAfunc.
DR InterPro; IPR006461; PLAC_motif_containing.
DR Pfam; PF19584; MCAfunc; 1.
DR Pfam; PF04749; PLAC8; 1.
DR TIGRFAMs; TIGR01571; A_thal_Cys_rich; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Coiled coil; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..416
FT /note="Protein MID1-COMPLEMENTING ACTIVITY 2"
FT /id="PRO_0000407742"
FT TRANSMEM 338..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT COILED 191..219
FT /evidence="ECO:0000255"
FT VAR_SEQ 307..416
FT /note="LKTLFFPCGTLAKISTVATSRQISSTEVCKNLIVYSLILSCCCYTCCIRKKL
FT RKTLNITGGCIDDFLSHLMCCCCALVQELREVEIHRASYGTEKSNKEMSPPTPQFMEE
FT -> KPSHSVFI (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_040965"
FT VAR_SEQ 398..399
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_040966"
FT VAR_SEQ 399..416
FT /note="TEKSNKEMSPPTPQFMEE -> KIYTFTDILTYF (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_040967"
SQ SEQUENCE 416 AA; 47414 MW; 1D9F308788EA9F42 CRC64;
MANSWDQLGE IASVAQLTGI DALKLIGMIV NAANTARMHK KNCRQFAHHL KLIRNLLEQI
KNSEMNQRSE ILEPLQGLDD ALRRSYILVK SCQEKSYLYL LAMGWNIVNQ FEKAQNEIDL
FLKIVPLINM ADNARIRERL EAIERDQREY TLDEEDRKVQ DVILKQESTR EAATSVLKKT
LSRSYPNMGF CEALKTEEEK LQLELQRSRA RYDADQCEVI QRLIDVTQTA ATVEPNLEKV
LTKKEELTSS KKRDDLYDTD SSSIRADSRS TSYVSSGHEL LSGRSLQHRG NWHADLLDCC
SEPCLCLKTL FFPCGTLAKI STVATSRQIS STEVCKNLIV YSLILSCCCY TCCIRKKLRK
TLNITGGCID DFLSHLMCCC CALVQELREV EIHRASYGTE KSNKEMSPPT PQFMEE
