ID   MCA5_ARATH              Reviewed;         410 AA.
AC   O64518;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Metacaspase-5;
DE            Short=AtMC5;
DE            EC=3.4.22.-;
DE   AltName: Full=Metacaspase 2b;
DE            Short=AtMCP2b;
DE   AltName: Full=Metacaspase-6;
GN   Name=AMC5; Synonyms=AMC6, MCP2B; OrderedLocusNames=At1g79330;
GN   ORFNames=YUP8H12R.5;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AUTOCATALYTIC CLEAVAGE, GENE FAMILY, AND
RP   NOMENCLATURE.
RX   PubMed=15326173; DOI=10.1074/jbc.m406329200;
RA   Vercammen D., van de Cotte B., De Jaeger G., Eeckhout D., Casteels P.,
RA   Vandepoele K., Vandenberghe I., van Beeumen J., Inze D., van Breusegem F.;
RT   "Type II metacaspases Atmc4 and Atmc9 of Arabidopsis thaliana cleave
RT   substrates after arginine and lysine.";
RL   J. Biol. Chem. 279:45329-45336(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Ikeda Y., Krishnamurthy N., Chua N.-H.;
RT   "Characterization of metacaspases.";
RL   Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   GENE FAMILY.
RX   PubMed=20565583; DOI=10.1111/j.1364-3703.2004.00206.x;
RA   Watanabe N., Lam E.;
RT   "Recent advance in the study of caspase-like proteases and Bax inhibitor-1
RT   in plants: their possible roles as regulator of programmed cell death.";
RL   Mol. Plant Pathol. 5:65-70(2004).
RN   [7]
RP   FUNCTION, AUTOCATALYTIC CLEAVAGE, AND MUTAGENESIS OF CYS-139.
RX   PubMed=15691845; DOI=10.1074/jbc.m413527200;
RA   Watanabe N., Lam E.;
RT   "Two Arabidopsis metacaspases AtMCP1b and AtMCP2b are arginine/lysine-
RT   specific cysteine proteases and activate apoptosis-like cell death in
RT   yeast.";
RL   J. Biol. Chem. 280:14691-14699(2005).
RN   [8]
RP   TISSUE SPECIFICITY.
RX   PubMed=21395887; DOI=10.1111/j.1365-313x.2011.04554.x;
RA   Watanabe N., Lam E.;
RT   "Arabidopsis metacaspase 2d is a positive mediator of cell death induced
RT   during biotic and abiotic stresses.";
RL   Plant J. 66:969-982(2011).
CC   -!- FUNCTION: Cysteine protease that cleaves specifically after arginine or
CC       lysine residues. Does not cleave caspase-specific substrates. May be
CC       involved in the modulation of programmed cell death activated by
CC       oxidative stress. {ECO:0000269|PubMed:15691845}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, leaves, cauline leaves and
CC       flower buds. {ECO:0000269|PubMed:21395887}.
CC   -!- PTM: Proteolytically processed; by an autocatalytic mechanism.
CC   -!- SIMILARITY: Belongs to the peptidase C14B family. {ECO:0000305}.
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DR   EMBL; AY219830; AAP44518.1; -; mRNA.
DR   EMBL; AY322533; AAP84714.1; -; mRNA.
DR   EMBL; AC002986; AAC17038.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE36231.1; -; Genomic_DNA.
DR   EMBL; BT029236; ABJ98568.1; -; mRNA.
DR   PIR; T01022; T01022.
DR   RefSeq; NP_178051.1; NM_106581.3.
DR   AlphaFoldDB; O64518; -.
DR   SMR; O64518; -.
DR   STRING; 3702.AT1G79330.1; -.
DR   MEROPS; C14.049; -.
DR   PaxDb; O64518; -.
DR   PRIDE; O64518; -.
DR   ProteomicsDB; 238370; -.
DR   EnsemblPlants; AT1G79330.1; AT1G79330.1; AT1G79330.
DR   GeneID; 844271; -.
DR   Gramene; AT1G79330.1; AT1G79330.1; AT1G79330.
DR   KEGG; ath:AT1G79330; -.
DR   Araport; AT1G79330; -.
DR   TAIR; locus:2207430; AT1G79330.
DR   eggNOG; KOG1546; Eukaryota.
DR   HOGENOM; CLU_029389_4_1_1; -.
DR   InParanoid; O64518; -.
DR   OMA; GHPEMAY; -.
DR   OrthoDB; 792090at2759; -.
DR   PhylomeDB; O64518; -.
DR   PRO; PR:O64518; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; O64518; baseline and differential.
DR   Genevisible; O64518; AT.
DR   GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IDA:TAIR.
DR   GO; GO:0043068; P:positive regulation of programmed cell death; IDA:TAIR.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Autocatalytic cleavage; Hydrolase; Protease; Reference proteome;
KW   S-nitrosylation; Thiol protease.
FT   CHAIN           1..410
FT                   /note="Metacaspase-5"
FT                   /id="PRO_0000334603"
FT   ACT_SITE        86
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        139
FT   MOD_RES         139
FT                   /note="S-nitrosocysteine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9FYE1"
FT   MUTAGEN         139
FT                   /note="C->A: Loss of autoprocessing."
FT                   /evidence="ECO:0000269|PubMed:15691845"
SQ   SEQUENCE   410 AA;  44846 MW;  02815A2F76DBD29A CRC64;
     MAKKAVLIGI NYPGTKAELR GCVNDVRRVH KSLVDRFGFS ERNITELIDT DESSTKPTGK
     NIRRALLNLV ESAKPGDVLV VHYSGHGTRL PAETGEDDDT GYDECIVPCD MNLITDDEFR
     DLVEKVPKEA HITIISDSCH SGGLIDEAKE QIGESTKKKP KKESGGSSGL GIKGFVREAV
     EEALESKGIA IPHHKDEKDE NKTKELKLED GAKVHVVNKS LPLQTLIDIL KQNTGNNDIE
     VGKIRPTLFN VFGEDASPKV KKFMKVILTK LQEGKTEGGI LGMIGKLAQE FLKHKLNDDE
     EYVKPAMKTH VGNKQEVYAG ASNGSLADNG ILISGCQTDQ TSADASPQGH PEMAYGAFTN
     AVQIILEETK GMITYKELVL KARKLLKKQG FSQRPGLYCS DSFVNAPFIC