MAGD4_HUMAN
ID MAGD4_HUMAN Reviewed; 741 AA.
AC Q96JG8; A8K093; Q5HYN6; Q8WXW4; Q9BQ84; Q9BVH1; Q9BYH3; Q9BYH4; Q9H217;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2004, sequence version 3.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Melanoma-associated antigen D4;
DE AltName: Full=MAGE-D4 antigen;
DE AltName: Full=MAGE-E1 antigen;
GN Name=MAGED4; Synonyms=KIAA1859, MAGED4A, MAGEE1;
GN and
GN Name=MAGED4B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), AND TISSUE SPECIFICITY.
RX PubMed=11406556;
RA Sasaki M., Nakahira K., Kawano Y., Katakura H., Yoshimine T., Shimizu K.,
RA Kim S.U., Ikenaka K.;
RT "MAGE-E1, a new member of the melanoma-associated antigen gene family and
RT its expression in human glioma.";
RL Cancer Res. 61:4809-4814(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 2 AND 3).
RX PubMed=11602350; DOI=10.1016/s0378-1119(01)00698-9;
RA Kawano Y., Sasaki M., Nakahira K., Yoshimine T., Shimizu K., Wada H.,
RA Ikenaka K.;
RT "Structural characterization and chromosomal localization of the MAGE-E1
RT gene.";
RL Gene 277:129-137(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Amygdala;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=11347906; DOI=10.1093/dnares/8.2.85;
RA Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XX. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 8:85-95(2001).
RN [5]
RP SEQUENCE REVISION.
RX PubMed=12168954; DOI=10.1093/dnares/9.3.99;
RA Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.;
RT "Construction of expression-ready cDNA clones for KIAA genes: manual
RT curation of 330 KIAA cDNA clones.";
RL DNA Res. 9:99-106(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
RC TISSUE=Brain, Eye, and PNS;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [10]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 60-741.
RA Zhang C.;
RT "Identification of a novel member of human Mage-D subfamily genes.";
RL Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
RN [11]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 497-627.
RC TISSUE=Testis;
RA Lucas S., Boon T.;
RT "Identification of new genes of the MAGE family.";
RL Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
RN [12]
RP TISSUE SPECIFICITY.
RX PubMed=16082191; DOI=10.4161/cbt.4.9.1907;
RA Kraemer B.F., Schoor O., Krueger T., Reichle C., Mueller M., Weinschenk T.,
RA Hennenlotter J., Stenzl A., Rammensee H.-G., Stevanovic S.;
RT "MAGED4-expression in renal cell carcinoma and identification of an HLA-
RT A*25-restricted MHC class I ligand from solid tumor tissue.";
RL Cancer Biol. Ther. 4:943-948(2005).
RN [13]
RP FUNCTION, AND INTERACTION WITH TRIM27.
RX PubMed=20864041; DOI=10.1016/j.molcel.2010.08.029;
RA Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.;
RT "MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.";
RL Mol. Cell 39:963-974(2010).
CC -!- FUNCTION: May enhance ubiquitin ligase activity of RING-type zinc
CC finger-containing E3 ubiquitin-protein ligases. Proposed to act through
CC recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at
CC the E3:substrate complex. {ECO:0000269|PubMed:20864041}.
CC -!- SUBUNIT: Interacts with TRIM27. {ECO:0000269|PubMed:20864041}.
CC -!- INTERACTION:
CC Q96JG8; Q8N111: CEND1; NbExp=3; IntAct=EBI-721864, EBI-946825;
CC Q96JG8; Q9NVL1-2: FAM86C1P; NbExp=3; IntAct=EBI-721864, EBI-12845222;
CC Q96JG8; Q0VD86: INCA1; NbExp=3; IntAct=EBI-721864, EBI-6509505;
CC Q96JG8; P80188: LCN2; NbExp=3; IntAct=EBI-721864, EBI-11911016;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1; Synonyms=MAGE-E1a;
CC IsoId=Q96JG8-1; Sequence=Displayed;
CC Name=2; Synonyms=MAGE-E1b;
CC IsoId=Q96JG8-2; Sequence=VSP_009287;
CC Name=3; Synonyms=MAGE-E1c;
CC IsoId=Q96JG8-3; Sequence=VSP_009288, VSP_009289;
CC Name=4;
CC IsoId=Q96JG8-4; Sequence=VSP_035013;
CC -!- TISSUE SPECIFICITY: Expressed only in brain and ovary among normal
CC tissues. Isoform 1 and isoform 2 are specifically expressed in glioma
CC cells among cancer cells. Detected in some renal cell carcinoma
CC samples. {ECO:0000269|PubMed:11406556, ECO:0000269|PubMed:16082191}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH01207.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAL50032.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAB47488.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB040527; BAB33378.1; -; mRNA.
DR EMBL; AB040528; BAB33379.1; -; mRNA.
DR EMBL; AB040529; BAB33380.1; -; mRNA.
DR EMBL; AB056059; BAB61778.1; -; Genomic_DNA.
DR EMBL; AB056059; BAB61779.1; -; Genomic_DNA.
DR EMBL; AB056059; BAB61780.1; -; Genomic_DNA.
DR EMBL; AL136582; CAB66517.1; -; mRNA.
DR EMBL; AB058762; BAB47488.2; ALT_INIT; mRNA.
DR EMBL; AK289458; BAF82147.1; -; mRNA.
DR EMBL; AL928717; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX537154; CAI41307.1; -; Genomic_DNA.
DR EMBL; CH471230; EAW62885.1; -; Genomic_DNA.
DR EMBL; BC001207; AAH01207.1; ALT_INIT; mRNA.
DR EMBL; BC063838; AAH63838.1; -; mRNA.
DR EMBL; BC081566; AAH81566.1; -; mRNA.
DR EMBL; AF329733; AAL50032.1; ALT_FRAME; mRNA.
DR EMBL; AF320908; AAG38604.1; -; mRNA.
DR CCDS; CCDS14338.1; -. [Q96JG8-1]
DR CCDS; CCDS35281.1; -. [Q96JG8-2]
DR CCDS; CCDS48116.1; -. [Q96JG8-2]
DR CCDS; CCDS56602.1; -. [Q96JG8-4]
DR CCDS; CCDS65261.1; -. [Q96JG8-4]
DR RefSeq; NP_001092270.1; NM_001098800.2. [Q96JG8-2]
DR RefSeq; NP_001229291.1; NM_001242362.1. [Q96JG8-4]
DR RefSeq; NP_001258990.1; NM_001272061.1. [Q96JG8-4]
DR RefSeq; NP_001258991.1; NM_001272062.1. [Q96JG8-1]
DR RefSeq; NP_001258992.1; NM_001272063.1. [Q96JG8-1]
DR RefSeq; NP_110428.2; NM_030801.3. [Q96JG8-1]
DR RefSeq; NP_803879.1; NM_177535.2. [Q96JG8-1]
DR RefSeq; NP_803881.1; NM_177537.2. [Q96JG8-2]
DR RefSeq; XP_006724670.1; XM_006724607.3.
DR RefSeq; XP_006724671.1; XM_006724608.3.
DR RefSeq; XP_011529124.1; XM_011530822.1.
DR RefSeq; XP_016885365.1; XM_017029876.1.
DR AlphaFoldDB; Q96JG8; -.
DR SMR; Q96JG8; -.
DR BioGRID; 123521; 24.
DR BioGRID; 608671; 3.
DR IntAct; Q96JG8; 15.
DR MINT; Q96JG8; -.
DR STRING; 9606.ENSP00000421861; -.
DR iPTMnet; Q96JG8; -.
DR PhosphoSitePlus; Q96JG8; -.
DR BioMuta; MAGED4B; -.
DR DMDM; 41017391; -.
DR EPD; Q96JG8; -.
DR jPOST; Q96JG8; -.
DR MassIVE; Q96JG8; -.
DR MaxQB; Q96JG8; -.
DR PaxDb; Q96JG8; -.
DR PeptideAtlas; Q96JG8; -.
DR PRIDE; Q96JG8; -.
DR ProteomicsDB; 76958; -. [Q96JG8-1]
DR ProteomicsDB; 76959; -. [Q96JG8-2]
DR ProteomicsDB; 76960; -. [Q96JG8-3]
DR ProteomicsDB; 76961; -. [Q96JG8-4]
DR Antibodypedia; 1173; 68 antibodies from 14 providers.
DR Antibodypedia; 26378; 27 antibodies from 10 providers.
DR DNASU; 81557; -.
DR Ensembl; ENST00000335504.10; ENSP00000335385.5; ENSG00000187243.17. [Q96JG8-1]
DR Ensembl; ENST00000360134.10; ENSP00000353252.6; ENSG00000187243.17. [Q96JG8-2]
DR Ensembl; ENST00000375626.7; ENSP00000364777.3; ENSG00000154545.17. [Q96JG8-2]
DR Ensembl; ENST00000471932.6; ENSP00000507908.1; ENSG00000154545.17. [Q96JG8-1]
DR Ensembl; ENST00000485287.5; ENSP00000421861.1; ENSG00000187243.17. [Q96JG8-4]
DR Ensembl; ENST00000497164.5; ENSP00000423848.1; ENSG00000187243.17. [Q96JG8-1]
DR Ensembl; ENST00000599522.7; ENSP00000472118.2; ENSG00000154545.17. [Q96JG8-4]
DR GeneID; 728239; -.
DR GeneID; 81557; -.
DR KEGG; hsa:728239; -.
DR KEGG; hsa:81557; -.
DR MANE-Select; ENST00000335504.10; ENSP00000335385.5; NM_030801.5; NP_110428.2.
DR MANE-Select; ENST00000471932.6; ENSP00000507908.1; NM_001272063.2; NP_001258992.1.
DR UCSC; uc004dpr.5; human. [Q96JG8-1]
DR CTD; 728239; -.
DR CTD; 81557; -.
DR DisGeNET; 728239; -.
DR DisGeNET; 81557; -.
DR GeneCards; MAGED4; -.
DR GeneCards; MAGED4B; -.
DR HGNC; HGNC:23793; MAGED4.
DR HGNC; HGNC:22880; MAGED4B.
DR HPA; ENSG00000154545; Tissue enriched (brain).
DR HPA; ENSG00000187243; Tissue enhanced (brain, pituitary gland).
DR MIM; 300702; gene.
DR MIM; 300765; gene.
DR neXtProt; NX_Q96JG8; -.
DR OpenTargets; ENSG00000154545; -.
DR PharmGKB; PA145148472; -.
DR VEuPathDB; HostDB:ENSG00000154545; -.
DR VEuPathDB; HostDB:ENSG00000187243; -.
DR eggNOG; KOG4562; Eukaryota.
DR GeneTree; ENSGT00940000162389; -.
DR HOGENOM; CLU_370025_0_0_1; -.
DR InParanoid; Q96JG8; -.
DR OMA; AEPWTRM; -.
DR OrthoDB; 1195799at2759; -.
DR PhylomeDB; Q96JG8; -.
DR TreeFam; TF352132; -.
DR PathwayCommons; Q96JG8; -.
DR SignaLink; Q96JG8; -.
DR BioGRID-ORCS; 728239; 74 hits in 594 CRISPR screens.
DR BioGRID-ORCS; 81557; 38 hits in 597 CRISPR screens.
DR ChiTaRS; MAGED4; human.
DR GeneWiki; MAGED4B; -.
DR Pharos; Q96JG8; Tbio.
DR PRO; PR:Q96JG8; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; Q96JG8; protein.
DR Bgee; ENSG00000154545; Expressed in cortical plate and 96 other tissues.
DR ExpressionAtlas; Q96JG8; baseline and differential.
DR Genevisible; Q96JG8; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.1200; -; 1.
DR Gene3D; 1.10.10.1210; -; 1.
DR InterPro; IPR037445; MAGE.
DR InterPro; IPR041898; MAGE_WH1.
DR InterPro; IPR041899; MAGE_WH2.
DR InterPro; IPR030091; MAGED4.
DR InterPro; IPR002190; MHD_dom.
DR PANTHER; PTHR11736; PTHR11736; 1.
DR PANTHER; PTHR11736:SF37; PTHR11736:SF37; 1.
DR Pfam; PF01454; MAGE; 1.
DR SMART; SM01373; MAGE; 1.
DR PROSITE; PS50838; MAGE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Reference proteome; Tumor antigen;
KW Ubl conjugation pathway.
FT CHAIN 1..741
FT /note="Melanoma-associated antigen D4"
FT /id="PRO_0000156728"
FT DOMAIN 413..611
FT /note="MAGE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00127"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 136..206
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 247..296
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 323..379
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 700..720
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 136..155
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 167..206
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 340..367
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 349..414
FT /note="SKHLDDEYESSEEERETPAVPPTWRASQPSLTVRAQLAPRPPMAPRSQIPSR
FT HVLCLPPRNVTLLQ -> VRSPCPLPPPHPLAPVLSFSSLSCSSPPSPLPLLPLFSSFP
FT SFSPHLPSPPLLSSQLVHVSPTQVC (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:11406556"
FT /id="VSP_009288"
FT VAR_SEQ 415..741
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:11406556"
FT /id="VSP_009289"
FT VAR_SEQ 557
FT /note="K -> KNPELREETPLSMAPSW (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_035013"
FT VAR_SEQ 740..741
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11406556"
FT /id="VSP_009287"
FT CONFLICT 314
FT /note="A -> V (in Ref. 6; BAF82147)"
FT /evidence="ECO:0000305"
FT CONFLICT 635
FT /note="Q -> R (in Ref. 10)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 741 AA; 81378 MW; 56B073324C12A8EC CRC64;
MAEGSFSVQS ESYSVEDMDE GSDEVGEEEM VEGNDYEEFG AFGGYGTLTS FDIHILRAFG
SLGPGLRILS NEPWELENPV LAQTLVEALQ LDPETLANET AARAANVARA AASNRAARAA
AAAARTAFSQ VVASHRVATP QVSGEDTQPT TYAAEAQGPT PEPPLASPQT SQMLVTSKMA
APEAPATSAQ SQTGSPAQEA ATEGPSSACA FSQAPCAREV DANRPSTAFL GQNDVFDFTQ
PAGVSGMAFP RPKRPAPAQE AATEGPSAAS GVPQTGPGRE VAATRPKTTK SGKALAKTRW
VEPQNVVAAA AAKAKMATSI PEPEGAAAAT AQHSAEPWAR MGGKRTKKSK HLDDEYESSE
EERETPAVPP TWRASQPSLT VRAQLAPRPP MAPRSQIPSR HVLCLPPRNV TLLQERANKL
VKYLMIKDYK KIPIKRADML KDVIREYDEH FPEIIERATY TLEKKFGIHL KEIDKEEHLY
ILVCTRDSSA RLLGKTKDTP RLSLLLVILG VIFMNGNRAS EAVLWEALRK MGLRPGVRHP
FLGDLRKLIT DDFVKQKYLE YKKIPNSNPP EYEFLWGLRA RHETSKMRVL RFIAQNQNRD
PREWKAHFLE AVDDAFKTMD VDMAEEHARA QMRAQMNIGD EALIGRWSWD DIQVELLTWD
EDGDFGDAWA RIPFAFWARY HQYILNSNRA NRRATWRAGV SSGTNGGAST SVLDGPSTSS
TIRTRNAARA GASFFSWIQH R
