MAGBI_HUMAN
ID MAGBI_HUMAN Reviewed; 343 AA.
AC Q96M61;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Melanoma-associated antigen B18;
DE AltName: Full=MAGE-B18 antigen;
GN Name=MAGEB18;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT SER-40.
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT SER-40.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, AND INTERACTION WITH LNX1.
RX PubMed=20864041; DOI=10.1016/j.molcel.2010.08.029;
RA Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.;
RT "MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.";
RL Mol. Cell 39:963-974(2010).
CC -!- FUNCTION: May enhance ubiquitin ligase activity of RING-type zinc
CC finger-containing E3 ubiquitin-protein ligases. Proposed to act through
CC recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at
CC the E3:substrate complex. {ECO:0000269|PubMed:20864041}.
CC -!- SUBUNIT: Interacts with LNX1. {ECO:0000269|PubMed:20864041}.
CC -!- INTERACTION:
CC Q96M61; Q8NI60: COQ8A; NbExp=3; IntAct=EBI-741835, EBI-745535;
CC Q96M61; Q06547: GABPB1; NbExp=3; IntAct=EBI-741835, EBI-618165;
CC Q96M61; Q53GS7: GLE1; NbExp=3; IntAct=EBI-741835, EBI-1955541;
CC Q96M61; P42858: HTT; NbExp=16; IntAct=EBI-741835, EBI-466029;
CC Q96M61; P13473-2: LAMP2; NbExp=3; IntAct=EBI-741835, EBI-21591415;
CC Q96M61; Q8TBB1: LNX1; NbExp=13; IntAct=EBI-741835, EBI-739832;
CC Q96M61; Q96PC5: MIA2; NbExp=3; IntAct=EBI-741835, EBI-1050253;
CC Q96M61; Q9Y371: SH3GLB1; NbExp=3; IntAct=EBI-741835, EBI-2623095;
CC Q96M61; Q15025: TNIP1; NbExp=5; IntAct=EBI-741835, EBI-357849;
CC Q96M61; P04637: TP53; NbExp=3; IntAct=EBI-741835, EBI-366083;
CC Q96M61; O76024: WFS1; NbExp=3; IntAct=EBI-741835, EBI-720609;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8BQR7}.
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DR EMBL; AK057361; BAB71450.1; -; mRNA.
DR EMBL; AC129850; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC029525; AAH29525.1; -; mRNA.
DR CCDS; CCDS14216.1; -.
DR RefSeq; NP_775970.1; NM_173699.3.
DR AlphaFoldDB; Q96M61; -.
DR SMR; Q96M61; -.
DR BioGRID; 130398; 9.
DR IntAct; Q96M61; 14.
DR STRING; 9606.ENSP00000314543; -.
DR iPTMnet; Q96M61; -.
DR PhosphoSitePlus; Q96M61; -.
DR BioMuta; MAGEB18; -.
DR DMDM; 296435512; -.
DR MassIVE; Q96M61; -.
DR MaxQB; Q96M61; -.
DR PaxDb; Q96M61; -.
DR PeptideAtlas; Q96M61; -.
DR PRIDE; Q96M61; -.
DR ProteomicsDB; 77299; -.
DR Antibodypedia; 24619; 272 antibodies from 25 providers.
DR DNASU; 286514; -.
DR Ensembl; ENST00000325250.2; ENSP00000314543.1; ENSG00000176774.6.
DR GeneID; 286514; -.
DR KEGG; hsa:286514; -.
DR MANE-Select; ENST00000325250.2; ENSP00000314543.1; NM_173699.4; NP_775970.2.
DR UCSC; uc004dbq.3; human.
DR CTD; 286514; -.
DR GeneCards; MAGEB18; -.
DR HGNC; HGNC:28515; MAGEB18.
DR HPA; ENSG00000176774; Not detected.
DR MIM; 301064; gene.
DR neXtProt; NX_Q96M61; -.
DR OpenTargets; ENSG00000176774; -.
DR PharmGKB; PA142671483; -.
DR VEuPathDB; HostDB:ENSG00000176774; -.
DR eggNOG; KOG4562; Eukaryota.
DR GeneTree; ENSGT00940000163449; -.
DR HOGENOM; CLU_039582_1_0_1; -.
DR InParanoid; Q96M61; -.
DR OMA; DPIRHCY; -.
DR OrthoDB; 1195799at2759; -.
DR PhylomeDB; Q96M61; -.
DR TreeFam; TF328505; -.
DR PathwayCommons; Q96M61; -.
DR SignaLink; Q96M61; -.
DR BioGRID-ORCS; 286514; 10 hits in 685 CRISPR screens.
DR GenomeRNAi; 286514; -.
DR Pharos; Q96M61; Tdark.
DR PRO; PR:Q96M61; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; Q96M61; protein.
DR Bgee; ENSG00000176774; Expressed in testis and 2 other tissues.
DR Genevisible; Q96M61; HS.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.1200; -; 1.
DR Gene3D; 1.10.10.1210; -; 1.
DR InterPro; IPR037445; MAGE.
DR InterPro; IPR021072; MAGE_N.
DR InterPro; IPR041898; MAGE_WH1.
DR InterPro; IPR041899; MAGE_WH2.
DR InterPro; IPR002190; MHD_dom.
DR PANTHER; PTHR11736; PTHR11736; 1.
DR Pfam; PF01454; MAGE; 1.
DR Pfam; PF12440; MAGE_N; 1.
DR SMART; SM01373; MAGE; 1.
DR SMART; SM01392; MAGE_N; 1.
DR PROSITE; PS50838; MAGE; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome; Tumor antigen; Ubl conjugation pathway.
FT CHAIN 1..343
FT /note="Melanoma-associated antigen B18"
FT /id="PRO_0000156719"
FT DOMAIN 107..306
FT /note="MAGE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00127"
FT REGION 1..102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 100..343
FT /note="Interaction with LNX1"
FT /evidence="ECO:0000269|PubMed:20864041"
FT REGION 313..343
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 63..92
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 328..343
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 40
FT /note="P -> S (in dbSNP:rs5944317)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_053500"
SQ SEQUENCE 343 AA; 38533 MW; 58549E2E1D285B14 CRC64;
MPRGQKSKLR AREKRHQARC ENQDLGATQA TVAEGESPSP AYLLFGDRPQ NLPAAETPSI
PEALQGAPST TNAIAPVSCS SNEGASSQDE KSLGSSREAE GWKEDPLNKK VVSLVHFLLQ
KYETKEPITK GDMIKFVIRK DKCHFNEILK RASEHMELAL GVDLKEVDPI RHYYAFFSKL
DLTYDETTSD EEKIPKTGLL MIALGVIFLN GNRAPEEAVW EIMNMMGVYA DRKHFLYGDP
RKVMTKDLVQ LKYLEYQQVP NSDPPRYEFL WGPRAHAETS KMKVLEFVAK IHDTVPSAFP
SCYEEALRDE EQRTQARAAA RAHTAAMANA RSRTTSSSFS HAK
