ID   LTND_ECOL6              Reviewed;         302 AA.
AC   A0A0H2VA68;
DT   10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   03-AUG-2022, entry version 25.
DE   RecName: Full=L-threonate dehydrogenase {ECO:0000303|PubMed:27402745};
DE            EC=1.1.1.411 {ECO:0000269|PubMed:27402745};
GN   Name=ltnD {ECO:0000303|PubMed:27402745};
GN   Synonyms=ygbJ {ECO:0000312|EMBL:AAN81746.1};
GN   OrderedLocusNames=c3297 {ECO:0000312|EMBL:AAN81746.1};
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=27402745; DOI=10.1073/pnas.1605546113;
RA   Zhang X., Carter M.S., Vetting M.W., San Francisco B., Zhao S.,
RA   Al-Obaidi N.F., Solbiati J.O., Thiaville J.J., de Crecy-Lagard V.,
RA   Jacobson M.P., Almo S.C., Gerlt J.A.;
RT   "Assignment of function to a domain of unknown function: DUF1537 is a new
RT   kinase family in catabolic pathways for acid sugars.";
RL   Proc. Natl. Acad. Sci. U.S.A. 113:E4161-E4169(2016).
CC   -!- FUNCTION: Catalyzes oxidation of L-threonate to 2-oxo-tetronate. Can
CC       use either NAD(+) or NADP(+) as cosubstrate, with a preference for
CC       NAD(+). {ECO:0000269|PubMed:27402745}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-threonate + NAD(+) = 2-dehydro-L-erythronate + H(+) + NADH;
CC         Xref=Rhea:RHEA:52548, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57561, ChEBI:CHEBI:57945, ChEBI:CHEBI:136669;
CC         EC=1.1.1.411; Evidence={ECO:0000269|PubMed:27402745};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.16 mM for NAD(+) {ECO:0000269|PubMed:27402745};
CC         KM=0.59 mM for NADP(+) {ECO:0000269|PubMed:27402745};
CC         Note=kcat is 28 sec(-1) with NAD(+) as cosubstrate. kcat is 3.5 sec(-
CC         1) with NADP(+) as cosubstrate. {ECO:0000269|PubMed:27402745};
CC   -!- SIMILARITY: Belongs to the HIBADH-related family. L-threonate
CC       dehydrogenase subfamily. {ECO:0000305}.
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DR   EMBL; AE014075; AAN81746.1; -; Genomic_DNA.
DR   RefSeq; WP_000847998.1; NC_004431.1.
DR   AlphaFoldDB; A0A0H2VA68; -.
DR   SMR; A0A0H2VA68; -.
DR   STRING; 199310.c3297; -.
DR   EnsemblBacteria; AAN81746; AAN81746; c3297.
DR   KEGG; ecc:c3297; -.
DR   eggNOG; COG2084; Bacteria.
DR   HOGENOM; CLU_035117_1_2_6; -.
DR   OMA; QMFMQAS; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:UniProt.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0016054; P:organic acid catabolic process; IEA:UniProt.
DR   Gene3D; 1.10.1040.10; -; 1.
DR   InterPro; IPR002204; 3-OH-isobutyrate_DH-rel_CS.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR013328; 6PGD_dom2.
DR   InterPro; IPR006115; 6PGDH_NADP-bd.
DR   InterPro; IPR015815; HIBADH-related.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR029154; NADP-bd.
DR   Pfam; PF14833; NAD_binding_11; 1.
DR   Pfam; PF03446; NAD_binding_2; 1.
DR   PIRSF; PIRSF000103; HIBADH; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00895; 3_HYDROXYISOBUT_DH; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; NAD; NADP; Oxidoreductase.
FT   CHAIN           1..302
FT                   /note="L-threonate dehydrogenase"
FT                   /id="PRO_0000439748"
FT   ACT_SITE        178
FT                   /evidence="ECO:0000250|UniProtKB:Q9I5I6"
FT   BINDING         7..35
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P31937"
FT   BINDING         102
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P31937"
FT   BINDING         246
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P31937"
SQ   SEQUENCE   302 AA;  30706 MW;  536848758AC4FF4C CRC64;
     MKTGSEFHVG IVGLGSMGMG AALSCVRAGL STWGADLNSN ACATLKEAGA CGVSDNAATF
     AEKLDALLVL VVNATQVKQV LFGEKGVAQH LKPGTAVMVS STIASADAQE IATALAGFGL
     EMLDAPVSGG AVKAANGEMT VMASGSDIAF ERLAPVLEAV AGKVYRIGSE PGLGSTVKII
     HQLLAGVHIA AGAEAMALAA RAGIPLDVMY DVVTNAAGNS WMFENRMRHV VDGDYTPHSA
     VDIFVKDLGL VADTAKALHF PLPLASTALN MFTSASNAGY GKEDDSAVIK IFSGITLPGA
     KS