ID   ARFA_MYCTO              Reviewed;         326 AA.
AC   P9WIU4; L0T818; P65593; Q10557;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Peptidoglycan-binding protein ArfA;
DE   AltName: Full=Outer membrane porin A;
DE   AltName: Full=Outer membrane protein A;
DE            Short=OmpATb;
DE   AltName: Full=Outer membrane protein ArfA;
GN   Name=arfA; Synonyms=ompA; OrderedLocusNames=MT0922;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Probably plays a role in ammonia secretion that neutralizes
CC       the medium at pH 5.5, although it does not play a direct role in
CC       ammonia transport. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=May bind Zn(2+) via residues in the BON domain. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}. Cell outer
CC       membrane {ECO:0000250}. Note=Does not have a cleavable signal sequence.
CC   -!- SIMILARITY: Belongs to the outer membrane OOP (TC 1.B.6) superfamily.
CC       ArfA family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK45169.1; -; Genomic_DNA.
DR   PIR; H70782; H70782.
DR   RefSeq; WP_003404684.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WIU4; -.
DR   BMRB; P9WIU4; -.
DR   SMR; P9WIU4; -.
DR   EnsemblBacteria; AAK45169; AAK45169; MT0922.
DR   KEGG; mtc:MT0922; -.
DR   PATRIC; fig|83331.31.peg.991; -.
DR   HOGENOM; CLU_836308_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   CDD; cd07185; OmpA_C-like; 1.
DR   Gene3D; 3.30.1330.60; -; 1.
DR   InterPro; IPR007055; BON_dom.
DR   InterPro; IPR006664; OMP_bac.
DR   InterPro; IPR006665; OmpA-like.
DR   InterPro; IPR006690; OMPA-like_CS.
DR   InterPro; IPR036737; OmpA-like_sf.
DR   Pfam; PF04972; BON; 1.
DR   Pfam; PF00691; OmpA; 1.
DR   PRINTS; PR01021; OMPADOMAIN.
DR   SUPFAM; SSF103088; SSF103088; 1.
DR   PROSITE; PS01068; OMPA_1; 1.
DR   PROSITE; PS51123; OMPA_2; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Cell wall; Disulfide bond; Ion transport; Membrane;
KW   Metal-binding; Secreted; Transmembrane; Transmembrane helix; Transport;
KW   Zinc.
FT   CHAIN           1..326
FT                   /note="Peptidoglycan-binding protein ArfA"
FT                   /id="PRO_0000427944"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          127..196
FT                   /note="BON"
FT   DOMAIN          212..326
FT                   /note="OmpA-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00473"
FT   REGION          1..73
FT                   /note="Required for protein translocation to the outer
FT                   membrane"
FT                   /evidence="ECO:0000250"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..20
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        208..250
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   326 AA;  33574 MW;  9E0D46ABCC179F4A CRC64;
     MASKAGLGQT PATTDARRTQ KFYRGSPGRP WLIGAVVIPL LIAAIGYGAF ERPQSVTGPT
     GVLPTLTPTS TRGASALSLS LLSISRSGNT VTLIGDFPDE AAKAALMTAL NGLLAPGVNV
     IDQIHVDPVV RSLDFSSAEP VFTASVPIPD FGLKVERDTV TLTGTAPSSE HKDAVKRAAT
     STWPDMKIVN NIEVTGQAPP GPPASGPCAD LQSAINAVTG GPIAFGNDGA SLIPADYEIL
     NRVADKLKAC PDARVTINGY TDNTGSEGIN IPLSAQRAKI VADYLVARGV AGDHIATVGL
     GSVNPIASNA TPEGRAKNRR VEIVVN