KRH1_DROME
ID KRH1_DROME Reviewed; 845 AA.
AC P08155; Q9VMH5;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 187.
DE RecName: Full=Krueppel homolog 1;
DE AltName: Full=Krueppel homologous protein 1;
GN Name=Kr-h1; Synonyms=Kr-h; ORFNames=CG9167;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA), FUNCTION, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=Oregon-R; TISSUE=Embryo, Larva, and Pupae;
RX PubMed=10772791; DOI=10.1006/dbio.2000.9687;
RA Pecasse F., Beck Y., Ruiz C., Richards G.;
RT "Kruppel-homolog, a stage-specific modulator of the prepupal ecdysone
RT response, is essential for Drosophila metamorphosis.";
RL Dev. Biol. 221:53-67(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 289-367.
RX PubMed=3096579; DOI=10.1016/0092-8674(86)90817-2;
RA Schuh R., Aicher W., Gaul U., Cote S., Preiss A., Maier D., Seifert E.,
RA Nauber U., Schroeder C., Kemler R., Jaeckle H.;
RT "A conserved family of nuclear proteins containing structural elements of
RT the finger protein encoded by Kruppel, a Drosophila segmentation gene.";
RL Cell 47:1025-1032(1986).
CC -!- FUNCTION: Plays a general role in the hierarchies of gene expression
CC leading to metamorphosis. {ECO:0000269|PubMed:10772791}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Beta;
CC IsoId=P08155-1; Sequence=Displayed;
CC Name=Alpha;
CC IsoId=P08155-2; Sequence=VSP_006830;
CC -!- DEVELOPMENTAL STAGE: Beta isoform is expressed during embryogenesis,
CC most abundant in midembryogenesis, and in adults. Alpha isoform is
CC expressed from embryogenesis to 8 hours after pupariation, major period
CC of expression is during second instar. {ECO:0000269|PubMed:10772791}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AJ005440; CAA06543.2; -; mRNA.
DR EMBL; AJ005441; CAA06544.2; -; mRNA.
DR EMBL; AE014134; AAF52343.2; -; Genomic_DNA.
DR EMBL; AE014134; AAG22417.1; -; Genomic_DNA.
DR EMBL; AY051816; AAK93240.1; -; mRNA.
DR EMBL; M14940; AAA28660.1; -; mRNA.
DR RefSeq; NP_477466.1; NM_058118.5. [P08155-1]
DR RefSeq; NP_477467.1; NM_058119.5. [P08155-2]
DR AlphaFoldDB; P08155; -.
DR SMR; P08155; -.
DR BioGRID; 60025; 13.
DR IntAct; P08155; 11.
DR STRING; 7227.FBpp0288736; -.
DR PaxDb; P08155; -.
DR PRIDE; P08155; -.
DR EnsemblMetazoa; FBtr0344437; FBpp0310809; FBgn0266450. [P08155-2]
DR EnsemblMetazoa; FBtr0344438; FBpp0310810; FBgn0266450. [P08155-1]
DR GeneID; 33861; -.
DR KEGG; dme:Dmel_CG45074; -.
DR CTD; 33861; -.
DR FlyBase; FBgn0266450; Kr-h1.
DR VEuPathDB; VectorBase:FBgn0266450; -.
DR eggNOG; KOG1721; Eukaryota.
DR HOGENOM; CLU_014679_0_1_1; -.
DR InParanoid; P08155; -.
DR PhylomeDB; P08155; -.
DR BioGRID-ORCS; 33861; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Kr-h1; fly.
DR GenomeRNAi; 33861; -.
DR PRO; PR:P08155; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0266450; Expressed in brain and 36 other tissues.
DR Genevisible; P08155; DM.
DR GO; GO:0005829; C:cytosol; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007552; P:metamorphosis; IMP:FlyBase.
DR GO; GO:0045316; P:negative regulation of compound eye photoreceptor development; IMP:FlyBase.
DR GO; GO:0010977; P:negative regulation of neuron projection development; IGI:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISS:FlyBase.
DR GO; GO:0035075; P:response to ecdysone; IMP:FlyBase.
DR GO; GO:0042594; P:response to starvation; IDA:FlyBase.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 5.
DR SMART; SM00355; ZnF_C2H2; 8.
DR SUPFAM; SSF57667; SSF57667; 4.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 8.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 8.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Metal-binding;
KW Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..845
FT /note="Krueppel homolog 1"
FT /id="PRO_0000046991"
FT ZN_FING 194..216
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 271..293
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 299..321
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 327..349
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 355..377
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 383..407
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 413..435
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 441..463
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 141..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 469..610
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 757..845
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 141..161
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 480..510
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 526..563
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 579..610
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 761..776
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 792..806
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..54
FT /note="Missing (in isoform Alpha)"
FT /evidence="ECO:0000303|PubMed:10772791"
FT /id="VSP_006830"
FT CONFLICT 313
FT /note="N -> D (in Ref. 5; AAA28660)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 845 AA; 91452 MW; A4D878E98DC8E372 CRC64;
MTESKNDTKS WAPKQIWIKD VLKKSGTELL DISKSPAKAV AVKKSPAKDS ATTKMVYYSA
NQLLIKTEQS SQAQFCLQVP PPLTATTTSV GLGVPPSGGQ QEHFELLQTP QQRQMQLQLQ
DQHQQEQQQF VSYQLAIQQH QKQQQQQQHE SITNAAPTAA PSAQRIKTEP VGGFPASAAV
VSQVRKPSAS KPQFKCDQCG MTFGSKSAHT SHTKSHSKNQ DLSLNGASGA GVAAPVSTAA
IELNDAGLPV GIPKSPTIKP LANVAAGADP YQCNVCQKTF AVPARLIRHY RTHTGERPFE
CEFCHKLFSV KENLQVHRRI HTKERPYKCD VCGRAFEHSG KLHRHMRIHT GERPHKCSVC
EKTFIQSGQL VIHMRTHTGE KPYKCPEPGC GKGFTCSKQL KVHSRTHTGE KPYHCDICFR
DFGYNHVLKL HRVQHYGSKC YKCTICDETF KNKKEMEAHI KGHANEVPDD EAEAAAASAA
ASTSAGSSAG SPSLQGVSSN SESSNHSPPS SPPATKKPRQ ARQPRVSKTV AATLSIPTSS
PLSPSSLSST YSPSASSMAS PPPTSAHYLP VQMEADALSR DSGVSSAQPA HSTYADEEPT
DLSMQQVQGQ LPESTVDYYQ APPSLLELQP QPAGLTINPA LLEAASIARR HDDNDDQVQD
EDVHAAAWQM MQLCRGHGSL PPTEQPAPSH QPQVPTLHVS DLAANYDDTH EATVLIEHFK
RGDLARHGLH KGYAPVPKYE SALPNPDVVR RVEAAIGLRS STESPERSSS PESDSLMMAD
RNVMTLPLRK RKHYMNKGDD GQVDSEKASG DGTSAAGGAA SVGAGDGPGS KVMRMSSVIQ
FAKAS
