ID   KKIH_LACLA              Reviewed;         262 AA.
AC   Q02149;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   27-APR-2001, sequence version 2.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Probable aminoglycoside 3'-phosphotransferase;
DE            EC=2.7.1.95;
DE   AltName: Full=Kanamycin kinase;
GN   Name=ymdC; OrderedLocusNames=LL1211; ORFNames=L33782;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NCDO 2118;
RX   PubMed=1400209; DOI=10.1128/jb.174.20.6571-6579.1992;
RA   Delorme C., Ehrlich S.D., Renault P.;
RT   "Histidine biosynthesis genes in Lactococcus lactis subsp. lactis.";
RL   J. Bacteriol. 174:6571-6579(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=7687248; DOI=10.1128/jb.175.14.4391-4399.1993;
RA   Delorme C., Godon J.-J., Ehrlich S.D., Renault P.;
RT   "Gene inactivation in Lactococcus lactis: histidine biosynthesis.";
RL   J. Bacteriol. 175:4391-4399(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + kanamycin A = ADP + H(+) + kanamycin 3'-phosphate;
CC         Xref=Rhea:RHEA:24256, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57909, ChEBI:CHEBI:58214, ChEBI:CHEBI:456216;
CC         EC=2.7.1.95;
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; U92974; AAB81906.1; -; Genomic_DNA.
DR   EMBL; AE005176; AAK05309.1; -; Genomic_DNA.
DR   PIR; C86776; C86776.
DR   PIR; H45734; H45734.
DR   RefSeq; NP_267367.1; NC_002662.1.
DR   RefSeq; WP_010905831.1; NC_002662.1.
DR   AlphaFoldDB; Q02149; -.
DR   SMR; Q02149; -.
DR   STRING; 272623.L33782; -.
DR   PaxDb; Q02149; -.
DR   EnsemblBacteria; AAK05309; AAK05309; L33782.
DR   KEGG; lla:L33782; -.
DR   PATRIC; fig|272623.7.peg.1308; -.
DR   eggNOG; COG3231; Bacteria.
DR   HOGENOM; CLU_073027_1_0_9; -.
DR   OMA; KSDRHID; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008910; F:kanamycin kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd05150; APH; 1.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR024165; Kan/Strep_kinase.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   Pfam; PF01636; APH; 1.
DR   PIRSF; PIRSF000706; Kanamycin_kin; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Kinase; Nucleotide-binding;
KW   Reference proteome; Transferase.
FT   CHAIN           1..262
FT                   /note="Probable aminoglycoside 3'-phosphotransferase"
FT                   /id="PRO_0000204813"
FT   ACT_SITE        187
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        164
FT                   /note="I -> T (in Ref. 1; AAB81906)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   262 AA;  30721 MW;  62661D98FAA15527 CRC64;
     MTNLKELRIN IEKFPEALHN TLKDAKIYDS SSSPEAQVLF IDKKDGYYLK IASSKTLERE
     AEMTAYFQKK KLGLGYISYL SDQSQDFLLK KKIQGENYLA KQYLNNPKRL CDNLAENLRF
     LHEQNFEDCP ILDHSERYLK KVEKNASINN SNLDFVNNYN IRTIEEAYDY IENKKLLLRN
     DTLLHGDYCL PNIILDNWKF KGFIDLDCAG VGDRHIDLFW GAWTLNFNIG TDQYRDRFFD
     AYGRDRIDVD RLKLVGCCEV FG