ID   KKA7_CAMJU              Reviewed;         250 AA.
AC   P14508;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Aminoglycoside 3'-phosphotransferase;
DE            EC=2.7.1.95;
DE   AltName: Full=APH(3')VII;
DE   AltName: Full=Kanamycin kinase, type VII;
DE   AltName: Full=Neomycin-kanamycin phosphotransferase type VII;
GN   Name=aphA-7;
OS   Campylobacter jejuni.
OG   Plasmid.
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Campylobacteraceae; Campylobacter.
OX   NCBI_TaxID=197;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PS1178;
RX   PubMed=2550983; DOI=10.1016/0147-619x(89)90035-8;
RA   Tenover F.C., Gilbert T., O'Hara P.;
RT   "Nucleotide sequence of a novel kanamycin resistance gene, aphA-7, from
RT   Campylobacter jejuni and comparison to other kanamycin phosphotransferase
RT   genes.";
RL   Plasmid 22:52-58(1989).
CC   -!- FUNCTION: Resistance to kanamycin and structurally-related
CC       aminoglycosides, including amikacin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + kanamycin A = ADP + H(+) + kanamycin 3'-phosphate;
CC         Xref=Rhea:RHEA:24256, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57909, ChEBI:CHEBI:58214, ChEBI:CHEBI:456216;
CC         EC=2.7.1.95;
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; M29953; AAA76822.1; -; Genomic_DNA.
DR   PIR; A43623; A43623.
DR   RefSeq; WP_063842175.1; NG_047447.1.
DR   AlphaFoldDB; P14508; -.
DR   SMR; P14508; -.
DR   PRIDE; P14508; -.
DR   KEGG; ag:AAA76822; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008910; F:kanamycin kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd05150; APH; 1.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR024165; Kan/Strep_kinase.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   Pfam; PF01636; APH; 1.
DR   PIRSF; PIRSF000706; Kanamycin_kin; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Kinase; Nucleotide-binding; Plasmid;
KW   Transferase.
FT   CHAIN           1..250
FT                   /note="Aminoglycoside 3'-phosphotransferase"
FT                   /id="PRO_0000204810"
FT   ACT_SITE        178
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   250 AA;  29688 MW;  AF3B7ADDE85531EC CRC64;
     MKYIDEIQIL GKCSEGMSPA EVYKCQLKNT VCYLKKIDDI FSKTTYSVKR EAEMMMWLSD
     KLKVPDVIEY GVREHSEYLI MSELRGKHID CFIDHPIKYI ECLVNALHQL QAIDIRNCPF
     SSKIDVRLKE LKYLLDNRIA DIDVSNWEDT TEFDDPMTLY QWLCENQPQE ELCLSHGDMS
     ANFFVSHDGI YFYDLARCGV ADKWLDIAFC VREIREYYPD SDYEKFFFNM LGLEPDYKKI
     NYYILLDEMF