KAKU4_ARATH
ID KAKU4_ARATH Reviewed; 485 AA.
AC Q949W6; F4JS05; O49592;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=Protein KAKU4 {ECO:0000303|PubMed:24824484};
GN Name=KAKU4 {ECO:0000303|PubMed:24824484};
GN OrderedLocusNames=At4g31430 {ECO:0000312|Araport:AT4G31430};
GN ORFNames=F3L17.8 {ECO:0000312|EMBL:CAB79861.1},
GN F8F16 {ECO:0000312|EMBL:CAA16546.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAK92781.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH CRWN1 AND CRWN4,
RP SUBCELLULAR LOCATION, AND NUCLEAR LOCALIZATION SIGNAL.
RX PubMed=24824484; DOI=10.1105/tpc.113.122168;
RA Goto C., Tamura K., Fukao Y., Shimada T., Hara-Nishimura I.;
RT "The novel nuclear envelope protein KAKU4 modulates nuclear morphology in
RT Arabidopsis.";
RL Plant Cell 26:2143-2155(2014).
CC -!- FUNCTION: Required for nucleus structure organization (e.g. size and
CC shape). {ECO:0000269|PubMed:24824484}.
CC -!- SUBUNIT: Core component of the LINC complex which is composed of inner
CC nuclear membrane SUN domain-containing proteins coupled to outer
CC nuclear membrane WIP proteins, the nucleoskeletal CRWN/LINC proteins,
CC and, possibly, KAKU4. Interacts with CRWN1 and CRWN4.
CC {ECO:0000269|PubMed:24824484}.
CC -!- SUBCELLULAR LOCATION: Nucleus inner membrane
CC {ECO:0000269|PubMed:24824484}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=KAKU4.1 {ECO:0000303|PubMed:24824484}, KAKU4.3
CC {ECO:0000303|PubMed:24824484};
CC IsoId=Q949W6-1; Sequence=Displayed;
CC Name=2; Synonyms=KAKU4.2 {ECO:0000303|PubMed:24824484};
CC IsoId=Q949W6-2; Sequence=VSP_057576;
CC -!- DISRUPTION PHENOTYPE: Aberrant nuclear morphology.
CC {ECO:0000269|PubMed:24824484}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA16546.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79861.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL021633; CAA16546.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161579; CAB79861.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85910.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85911.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85912.1; -; Genomic_DNA.
DR EMBL; AY050846; AAK92781.1; -; mRNA.
DR EMBL; AY142592; AAN13161.1; -; mRNA.
DR PIR; T04510; T04510.
DR RefSeq; NP_001031764.1; NM_001036687.1. [Q949W6-1]
DR RefSeq; NP_567876.1; NM_119292.1. [Q949W6-1]
DR RefSeq; NP_974653.1; NM_202924.2. [Q949W6-2]
DR AlphaFoldDB; Q949W6; -.
DR IntAct; Q949W6; 6.
DR STRING; 3702.AT4G31430.2; -.
DR iPTMnet; Q949W6; -.
DR PaxDb; Q949W6; -.
DR PRIDE; Q949W6; -.
DR ProteomicsDB; 250611; -. [Q949W6-1]
DR EnsemblPlants; AT4G31430.1; AT4G31430.1; AT4G31430. [Q949W6-1]
DR EnsemblPlants; AT4G31430.2; AT4G31430.2; AT4G31430. [Q949W6-2]
DR EnsemblPlants; AT4G31430.3; AT4G31430.3; AT4G31430. [Q949W6-1]
DR GeneID; 829270; -.
DR Gramene; AT4G31430.1; AT4G31430.1; AT4G31430. [Q949W6-1]
DR Gramene; AT4G31430.2; AT4G31430.2; AT4G31430. [Q949W6-2]
DR Gramene; AT4G31430.3; AT4G31430.3; AT4G31430. [Q949W6-1]
DR KEGG; ath:AT4G31430; -.
DR Araport; AT4G31430; -.
DR TAIR; locus:2128176; AT4G31430.
DR eggNOG; ENOG502QPXJ; Eukaryota.
DR OMA; EIHGDDM; -.
DR OrthoDB; 507535at2759; -.
DR PRO; PR:Q949W6; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q949W6; baseline and differential.
DR Genevisible; Q949W6; AT.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0005635; C:nuclear envelope; IDA:TAIR.
DR GO; GO:0005637; C:nuclear inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0071763; P:nuclear membrane organization; IMP:TAIR.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Membrane; Nucleus; Reference proteome.
FT CHAIN 1..485
FT /note="Protein KAKU4"
FT /id="PRO_0000432818"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 74..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 249..270
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 364..404
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 444..485
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 22..25
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000303|PubMed:24824484"
FT COMPBIAS 80..103
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 369..404
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 453..485
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT VAR_SEQ 465..485
FT /note="GYGRSTKTHTTRRNQGFSAYS -> SPAVMEGQPKPTPPEETKASQPIAEIH
FT GDDMTVGNGSDGAINNESNDSHSSASNSSSTHEEEWLPGDQSLPNSNSASSSPGNTKVL
FT AYSRRGRGRGRGRGRGGRGKGRAK (in isoform 2)"
FT /id="VSP_057576"
SQ SEQUENCE 485 AA; 52148 MW; A7FD825621BD35E5 CRC64;
MDSVSGYAAG AGQPRVGGKI VRPRRTAVVR TPCERPVQRS RDPPQQNPSW ISRLVYKPAS
VIASGAGKFI SSVVFSDSSS SSEEDEDSSS DIDGDEDVEK NNPDFTEEDL LSAQQPSIQR
LSSKRVIEQL LLQETFAREE GDRLIDIIKA RVVDHPSVPS AIETSHDDYG LTSDVNVGEM
SNTAVMEARK WLEEKKSGSS SKYKATEDGA GSPVDVAKSY MRARLPWGSP AANNLDFRSP
SSARVQGTPL PYSAGNFSSS KLKRKSGSNQ SWNIQDEIRK VRAKATEEML KSPSSVASLE
PKYSPYVLAT DMLKGNASSL NADGAVRNEQ SRALPNSAIP TSEHNQTTEA NQAVKETGVL
HTRSRGVGLE ETFISTQGVK PSEDTNTAPQ SGTAVDDFND QDGDFIQPTS TIGNTTNAVL
ALGATLDPTG NSCIPKDVFE TSKEADEIGA SRHTSNGFPS SSPSGYGRST KTHTTRRNQG
FSAYS
