ID   KAIA_SYNP2              Reviewed;         299 AA.
AC   Q6L8L7; B1XMW2;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 2.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Circadian clock protein KaiA;
GN   Name=kaiA; OrderedLocusNames=SYNPCC7002_A0289;
OS   Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum
OS   quadruplicatum).
OC   Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus;
OC   unclassified Synechococcus.
OX   NCBI_TaxID=32049;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15170179; DOI=10.1038/nsmb781;
RA   Uzumaki T., Fujita M., Nakatsu T., Hayashi F., Shibata H., Itoh N.,
RA   Kato H., Ishiura M.;
RT   "Crystal structure of the C-terminal clock-oscillator domain of the
RT   cyanobacterial KaiA protein.";
RL   Nat. Struct. Mol. Biol. 11:623-631(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RA   Li T., Zhao J., Zhao C., Liu Z., Zhao F., Marquardt J., Nomura C.T.,
RA   Persson S., Detter J.C., Richardson P.M., Lanz C., Schuster S.C., Wang J.,
RA   Li S., Huang X., Cai T., Yu Z., Luo J., Zhao J., Bryant D.A.;
RT   "Complete sequence of Synechococcus sp. PCC 7002.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the KaiABC clock protein complex, which
CC       constitutes the main circadian regulator in cyanobacteria. The KaiABC
CC       complex may act as a promoter-nonspecific transcription regulator that
CC       represses transcription, possibly by acting on the state of chromosome
CC       compaction. In the complex, it enhances the phosphorylation status of
CC       KaiC. In contrast, the presence of KaiB in the complex decreases the
CC       phosphorylation status of KaiC, suggesting that KaiB acts by
CC       antagonizing the interaction between KaiA and KaiC. A KaiA dimer is
CC       sufficient to enhance KaiC hexamer phosphorylation (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. Component of the KaiABC complex, at least composed
CC       of a KaiC homohexamer, a KaiB dimer and two KaiA dimers. The KaiABC
CC       complex also interacts with SasA (By similarity). {ECO:0000250}.
CC   -!- DOMAIN: The KaiA domain mediates the interaction with KaiC, the
CC       homodimerization, and is responsible for the clock oscillation
CC       function. {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAD21211.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AB120711; BAD21211.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; CP000951; ACA98299.1; -; Genomic_DNA.
DR   RefSeq; WP_012305923.1; NC_010475.1.
DR   AlphaFoldDB; Q6L8L7; -.
DR   SMR; Q6L8L7; -.
DR   STRING; 32049.SYNPCC7002_A0289; -.
DR   EnsemblBacteria; ACA98299; ACA98299; SYNPCC7002_A0289.
DR   KEGG; syp:SYNPCC7002_A0289; -.
DR   eggNOG; ENOG502Z8HQ; Bacteria.
DR   HOGENOM; CLU_911234_0_0_3; -.
DR   OMA; VMAHLCE; -.
DR   Proteomes; UP000001688; Chromosome.
DR   GO; GO:0007623; P:circadian rhythm; IEA:InterPro.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR   Gene3D; 1.10.1240.30; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR011648; Circadian_clock_KaiA.
DR   InterPro; IPR020844; Circadian_clock_KaiA_N.
DR   InterPro; IPR020856; Circadian_clock_protein_KaiA_C.
DR   InterPro; IPR017944; KaiA/RbsU_helical_domain_sf.
DR   Pfam; PF07688; KaiA; 1.
DR   SMART; SM01247; KaiA; 1.
DR   SUPFAM; SSF101215; SSF101215; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51431; KAIA_C; 1.
DR   PROSITE; PS51430; KAIA_N; 1.
PE   3: Inferred from homology;
KW   Biological rhythms; Reference proteome.
FT   CHAIN           1..299
FT                   /note="Circadian clock protein KaiA"
FT                   /id="PRO_0000217870"
FT   DOMAIN          1..169
FT                   /note="KaiA N-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00760"
FT   DOMAIN          179..287
FT                   /note="KaiA C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00761"
FT   CONFLICT        253
FT                   /note="L -> F (in Ref. 1; BAD21211)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        264
FT                   /note="L -> I (in Ref. 1; BAD21211)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   299 AA;  34717 MW;  4A0D61FBD6C607F7 CRC64;
     MVSKLSLYLV TPPGFQPGKI EAQLIGDRYQ IKLLDYGQKF LAFLEANKEQ IDCLVVVQGK
     ENQDYFECLT QSGILLPCVF LGPTTACEIS QEDVSEQLYH SAEKYLDFAN LENLSLTIDQ
     AIAQFLHLAP SCALSDKPQD PHHSDPDKTH QAFLLLQQRR LAEKLRERLG YLGVYYKRNP
     KYFYRSLSPE EQQEFREQFV ADYRDIVLSY FSGDLPTNQA IDQFVNQAFF ADVSVSYILE
     THMKLMDEFA QQLKLEGRSE EILLDYRLTL IDIVAHLCEM YRRSIPREDL PFELLFRID