KAIA_SYNE7
ID KAIA_SYNE7 Reviewed; 284 AA.
AC Q79PF6; Q31NX1; Q9Z3H4;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Circadian clock protein KaiA;
GN Name=kaiA; OrderedLocusNames=Synpcc7942_1218; ORFNames=see0009;
OS Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans
OS R2).
OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX NCBI_TaxID=1140;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND MUTANTS KAIA1; KAIA2 AND
RP KAIA3.
RX PubMed=9727980; DOI=10.1126/science.281.5382.1519;
RA Ishiura M., Kutsuna S., Aoki S., Iwasaki H., Andersson C.R., Tanabe A.,
RA Golden S.S., Johnson C.H., Kondo T.;
RT "Expression of a gene cluster kaiABC as a circadian feedback process in
RT cyanobacteria.";
RL Science 281:1519-1523(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Holtman C.K., Sandoval P., Chen Y., Socias T., Mohler B.J., McMurtry S.,
RA Gonzalez A., Salinas I., Golden S.S., Youderian P.;
RT "Synechococcus elongatus PCC7942 cosmid 7G3.";
RL Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7942 / FACHB-805;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA Kyrpides N., Lykidis A., Richardson P.;
RT "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP INTERACTION WITH KAIC AND KAIB, AND MUTANT KAIA1.
RX PubMed=10064581; DOI=10.1093/emboj/18.5.1137;
RA Iwasaki H., Taniguchi Y., Ishiura M., Kondo T.;
RT "Physical interactions among circadian clock proteins KaiA, KaiB and KaiC
RT in cyanobacteria.";
RL EMBO J. 18:1137-1145(1999).
RN [5]
RP INTERACTION WITH KAIC, AND MUTANTS KAIA2 AND KAIA4.
RX PubMed=11356188; DOI=10.1016/s0014-5793(01)02408-5;
RA Taniguchi Y., Yamaguchi A., Hijikata A., Iwasaki H., Kamagata K.,
RA Ishiura M., Go M., Kondo T.;
RT "Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC.";
RL FEBS Lett. 496:86-90(2001).
RN [6]
RP MUTAGENESIS OF ILE-9; ILE-16; LEU-31; SER-36; CYS-53; VAL-76; GLN-113;
RP GLN-117; ASP-119; VAL-131; ASP-136; PHE-178; ARG-180; MET-194; PHE-224;
RP PHE-225; GLU-239; MET-241; ASP-242; GLU-243; PHE-244; ALA-245; ILE-266 AND
RP CYS-273.
RX PubMed=12213935; DOI=10.1099/00221287-148-9-2903;
RA Nishimura H., Nakahira Y., Imai K., Tsuruhara A., Kondo H., Hayashi H.,
RA Hirai M., Saito H., Kondo T.;
RT "Mutations in KaiA, a clock protein, extend the period of circadian rhythm
RT in the cyanobacterium Synechococcus elongatus PCC 7942.";
RL Microbiology 148:2903-2909(2002).
RN [7]
RP FUNCTION.
RX PubMed=12391300; DOI=10.1073/pnas.222467299;
RA Iwasaki H., Nishiwaki T., Kitayama Y., Nakajima M., Kondo T.;
RT "KaiA-stimulated KaiC phosphorylation in circadian timing loops in
RT cyanobacteria.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:15788-15793(2002).
RN [8]
RP FUNCTION.
RX PubMed=12727878; DOI=10.1093/emboj/cdg168;
RA Xu Y., Mori T., Johnson C.H.;
RT "Cyanobacterial circadian clockwork: roles of KaiA, KaiB and the kaiBC
RT promoter in regulating KaiC.";
RL EMBO J. 22:2117-2126(2003).
RN [9]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=12727879; DOI=10.1093/emboj/cdg212;
RA Kitayama Y., Iwasaki H., Nishiwaki T., Kondo T.;
RT "KaiB functions as an attenuator of KaiC phosphorylation in the
RT cyanobacterial circadian clock system.";
RL EMBO J. 22:2127-2134(2003).
RN [10]
RP STOICHIOMETRY OF THE COMPLEX FORMED WITH KAIC.
RX PubMed=15003530; DOI=10.1016/j.bbrc.2004.02.034;
RA Hayashi F., Ito H., Fujita M., Iwase R., Uzumaki T., Ishiura M.;
RT "Stoichiometric interactions between cyanobacterial clock proteins KaiA and
RT KaiC.";
RL Biochem. Biophys. Res. Commun. 316:195-202(2004).
RN [11]
RP STRUCTURE BY NMR OF 1-135.
RX PubMed=12438647; DOI=10.1073/pnas.232517099;
RA Williams S.B., Vakonakis I., Golden S.S., LiWang A.C.;
RT "Structure and function from the circadian clock protein KaiA of
RT Synechococcus elongatus: a potential clock input mechanism.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:15357-15362(2002).
RN [12]
RP ERRATUM OF PUBMED:12438647.
RA Williams S.B., Vakonakis I., Golden S.S., LiWang A.C.;
RL Proc. Natl. Acad. Sci. U.S.A. 100:763-763(2003).
RN [13]
RP X-RAY CRYSTALLOGRAPHY (2.03 ANGSTROMS) OF 2-284, HOMODIMERIZATION, AND
RP DOMAIN.
RX PubMed=15007067; DOI=10.1074/jbc.m400077200;
RA Ye S., Vakonakis I., Ioerger T.R., LiWang A.C., Sacchettini J.C.;
RT "Crystal structure of circadian clock protein KaiA from Synechococcus
RT elongatus.";
RL J. Biol. Chem. 279:20511-20518(2004).
CC -!- FUNCTION: Component of the KaiABC clock protein complex, which
CC constitutes the main circadian regulator in cyanobacteria. The KaiABC
CC complex may act as a promoter-nonspecific transcription regulator that
CC represses transcription, possibly by acting on the state of chromosome
CC compaction. In the complex, it enhances the phosphorylation status of
CC KaiC. In contrast, the presence of KaiB in the complex decreases the
CC phosphorylation status of KaiC, suggesting that KaiB acts by
CC antagonizing the interaction between KaiA and KaiC. A KaiA dimer is
CC sufficient to enhance KaiC hexamer phosphorylation.
CC {ECO:0000269|PubMed:12391300, ECO:0000269|PubMed:12727878,
CC ECO:0000269|PubMed:12727879, ECO:0000269|PubMed:9727980}.
CC -!- SUBUNIT: Homodimer. Component of the KaiABC complex, at least composed
CC of a KaiC homohexamer, a KaiB dimer and two KaiA dimers. The KaiABC
CC complex also interacts with SasA. {ECO:0000269|PubMed:10064581,
CC ECO:0000269|PubMed:11356188}.
CC -!- INTERACTION:
CC Q79PF6; Q79PF6: kaiA; NbExp=5; IntAct=EBI-592281, EBI-592281;
CC Q79PF6; Q79PF5: kaiB; NbExp=11; IntAct=EBI-592281, EBI-619150;
CC Q79PF6; Q79PF4: kaiC; NbExp=22; IntAct=EBI-592281, EBI-592287;
CC Q79PF6; Q8GLI4: ldpA; NbExp=2; IntAct=EBI-592281, EBI-626836;
CC -!- DEVELOPMENTAL STAGE: Expressed constantly throughout the circadian
CC cycle. {ECO:0000269|PubMed:12727879}.
CC -!- DOMAIN: The N-terminal part (1-102), which is also called pseudo-
CC receiver domain, does not enhance KaiC autophosphorylation. It may have
CC an amplitude-amplifier function and may be involved in transducing
CC input signals to the KaiABC complex. This domain is not conserved in
CC other species. {ECO:0000269|PubMed:15007067}.
CC -!- DOMAIN: The KaiA domain mediates the interaction with KaiC, the
CC homodimerization, and is responsible for the clock oscillation
CC function. {ECO:0000269|PubMed:15007067}.
CC -!- MISCELLANEOUS: 'Kai' means 'cycle' in Japanese.
CC -!- CAUTION: PubMed:15007067 structure is derived from this sequence and
CC not from the one of S.elongatus. {ECO:0000305}.
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DR EMBL; AB010691; BAA37101.1; -; Genomic_DNA.
DR EMBL; AY120853; AAM82684.1; -; Genomic_DNA.
DR EMBL; CP000100; ABB57248.1; -; Genomic_DNA.
DR PIR; T44267; T44267.
DR RefSeq; WP_011377921.1; NC_007604.1.
DR PDB; 1M2E; NMR; -; A=1-135.
DR PDB; 1M2F; NMR; -; A=1-135.
DR PDB; 1R8J; X-ray; 2.03 A; A/B=2-284.
DR PDB; 4G86; X-ray; 2.39 A; A/B=2-284.
DR PDB; 5C5E; X-ray; 2.82 A; A/B=1-284.
DR PDB; 5N8Y; EM; 4.70 A; M/N/O/P/Q/R/S/T/U/V/W/X=1-284.
DR PDBsum; 1M2E; -.
DR PDBsum; 1M2F; -.
DR PDBsum; 1R8J; -.
DR PDBsum; 4G86; -.
DR PDBsum; 5C5E; -.
DR PDBsum; 5N8Y; -.
DR AlphaFoldDB; Q79PF6; -.
DR BMRB; Q79PF6; -.
DR SASBDB; Q79PF6; -.
DR SMR; Q79PF6; -.
DR DIP; DIP-33329N; -.
DR IntAct; Q79PF6; 4.
DR STRING; 1140.Synpcc7942_1218; -.
DR PRIDE; Q79PF6; -.
DR EnsemblBacteria; ABB57248; ABB57248; Synpcc7942_1218.
DR KEGG; syf:Synpcc7942_1218; -.
DR eggNOG; ENOG502Z8HQ; Bacteria.
DR HOGENOM; CLU_911234_0_0_3; -.
DR OMA; VMAHLCE; -.
DR OrthoDB; 1326064at2; -.
DR BioCyc; SYNEL:SYNPCC7942_1218-MON; -.
DR EvolutionaryTrace; Q79PF6; -.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0007623; P:circadian rhythm; IEA:InterPro.
DR GO; GO:0009649; P:entrainment of circadian clock; IDA:UniProtKB.
DR GO; GO:0035308; P:negative regulation of protein dephosphorylation; IMP:CACAO.
DR GO; GO:0042753; P:positive regulation of circadian rhythm; IDA:UniProtKB.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR Gene3D; 1.10.1240.30; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR011648; Circadian_clock_KaiA.
DR InterPro; IPR020844; Circadian_clock_KaiA_N.
DR InterPro; IPR020856; Circadian_clock_protein_KaiA_C.
DR InterPro; IPR017944; KaiA/RbsU_helical_domain_sf.
DR Pfam; PF07688; KaiA; 1.
DR SMART; SM01247; KaiA; 1.
DR SUPFAM; SSF101215; SSF101215; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51431; KAIA_C; 1.
DR PROSITE; PS51430; KAIA_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Biological rhythms.
FT CHAIN 1..284
FT /note="Circadian clock protein KaiA"
FT /id="PRO_0000217871"
FT DOMAIN 1..164
FT /note="KaiA N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00760"
FT DOMAIN 174..282
FT /note="KaiA C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00761"
FT MUTAGEN 9
FT /note="I->T: Extends the period of the circadian rhythm to
FT 29 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 16
FT /note="I->F: Extends the period of the circadian rhythm to
FT 27 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 31
FT /note="L->P: Extends the period of the circadian rhythm to
FT 27 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 36
FT /note="S->P: Extends the period of the circadian rhythm to
FT 29 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 53
FT /note="C->S: Induces an arrhythmic phenotype."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 76
FT /note="V->G: Extends the period of the circadian rhythm to
FT 28 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 103
FT /note="E->K: In kaiA1; extends the period of the circadian
FT rhythm to 33 hours and increases the interaction with
FT KaiB."
FT MUTAGEN 113
FT /note="Q->R: Extends the period of the circadian rhythm to
FT 33 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 117
FT /note="Q->L: Extends the period of the circadian rhythm to
FT 26 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 119
FT /note="D->E: Extends the period of the circadian rhythm to
FT 30 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 119
FT /note="D->G: Extends the period of the circadian rhythm to
FT 26 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 131
FT /note="V->A: Extends the period of the circadian rhythm to
FT 28 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 136
FT /note="D->V: Extends the period of the circadian rhythm to
FT 30 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 136
FT /note="D->Y: Extends the period of the circadian rhythm to
FT 29 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 166
FT /note="Y->C: In kaiA3; induces an arrhythmic phenotype and
FT reduces the interaction with KaiC."
FT MUTAGEN 178
FT /note="F->S,I: Induces an arrhythmic phenotype."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 180
FT /note="R->H: Extends the period of the circadian rhythm to
FT 26 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 194
FT /note="M->T: Extends the period of the circadian rhythm to
FT 26 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 224
FT /note="F->S: Induces an arrhythmic phenotype."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 225
FT /note="F->S: Induces an arrhythmic phenotype."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 239
FT /note="E->G: Extends the period of the circadian rhythm to
FT 28 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 241
FT /note="M->T: Extends the period of the circadian rhythm to
FT 38 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 242
FT /note="D->V,G: Extends the period of the circadian rhythm
FT to 27 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 243
FT /note="E->A: Extends the period of the circadian rhythm to
FT 34 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 244
FT /note="F->V: Extends the period of the circadian rhythm to
FT 26 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 245
FT /note="A->D: Extends the period of the circadian rhythm to
FT 26 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 249
FT /note="R->H: In kaiA1; extends the period of the circadian
FT rhythm to 30 hours."
FT MUTAGEN 266
FT /note="I->V: Extends the period of the circadian rhythm to
FT 27 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 273
FT /note="C->Y: Extends the period of the circadian rhythm to
FT 30 hours."
FT /evidence="ECO:0000269|PubMed:12213935"
FT MUTAGEN 274
FT /note="E->K: In kaiA3; induces an arrhythmic phenotype."
FT STRAND 5..10
FT /evidence="ECO:0007829|PDB:1R8J"
FT HELIX 14..23
FT /evidence="ECO:0007829|PDB:1R8J"
FT STRAND 29..34
FT /evidence="ECO:0007829|PDB:1R8J"
FT HELIX 37..46
FT /evidence="ECO:0007829|PDB:1R8J"
FT TURN 47..49
FT /evidence="ECO:0007829|PDB:4G86"
FT STRAND 52..57
FT /evidence="ECO:0007829|PDB:1R8J"
FT HELIX 63..72
FT /evidence="ECO:0007829|PDB:1R8J"
FT STRAND 79..83
FT /evidence="ECO:0007829|PDB:1R8J"
FT STRAND 87..89
FT /evidence="ECO:0007829|PDB:4G86"
FT STRAND 98..100
FT /evidence="ECO:0007829|PDB:1R8J"
FT STRAND 104..106
FT /evidence="ECO:0007829|PDB:1R8J"
FT HELIX 108..110
FT /evidence="ECO:0007829|PDB:1M2E"
FT HELIX 111..113
FT /evidence="ECO:0007829|PDB:1R8J"
FT HELIX 114..128
FT /evidence="ECO:0007829|PDB:1R8J"
FT TURN 134..136
FT /evidence="ECO:0007829|PDB:1R8J"
FT STRAND 138..140
FT /evidence="ECO:0007829|PDB:4G86"
FT TURN 144..147
FT /evidence="ECO:0007829|PDB:4G86"
FT HELIX 149..151
FT /evidence="ECO:0007829|PDB:1R8J"
FT HELIX 152..164
FT /evidence="ECO:0007829|PDB:1R8J"
FT STRAND 165..171
FT /evidence="ECO:0007829|PDB:1R8J"
FT HELIX 175..177
FT /evidence="ECO:0007829|PDB:1R8J"
FT TURN 179..181
FT /evidence="ECO:0007829|PDB:1R8J"
FT HELIX 184..205
FT /evidence="ECO:0007829|PDB:1R8J"
FT STRAND 208..211
FT /evidence="ECO:0007829|PDB:5C5E"
FT HELIX 212..225
FT /evidence="ECO:0007829|PDB:1R8J"
FT HELIX 230..249
FT /evidence="ECO:0007829|PDB:1R8J"
FT TURN 250..252
FT /evidence="ECO:0007829|PDB:4G86"
FT HELIX 257..261
FT /evidence="ECO:0007829|PDB:1R8J"
FT HELIX 262..279
FT /evidence="ECO:0007829|PDB:1R8J"
SQ SEQUENCE 284 AA; 32632 MW; 7C4F3B79FFDF59A2 CRC64;
MLSQIAICIW VESTAILQDC QRALSADRYQ LQVCESGEML LEYAQTHRDQ IDCLILVAAN
PSFRAVVQQL CFEGVVVPAI VVGDRDSEDP DEPAKEQLYH SAELHLGIHQ LEQLPYQVDA
ALAEFLRLAP VETMADHIML MGANHDPELS SQQRDLAQRL QERLGYLGVY YKRDPDRFLR
NLPAYESQKL HQAMQTSYRE IVLSYFSPNS NLNQSIDNFV NMAFFADVPV TKVVEIHMEL
MDEFAKKLRV EGRSEDILLD YRLTLIDVIA HLCEMYRRSI PRET
