ID   ISPH_BUCBP              Reviewed;         221 AA.
AC   Q89AV1;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Putative 4-hydroxy-3-methylbut-2-enyl diphosphate reductase homolog;
GN   Name=ispH; Synonyms=lytB; OrderedLocusNames=bbp_137;
OS   Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=224915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bp;
RX   PubMed=12522265; DOI=10.1073/pnas.0235981100;
RA   van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F.,
RA   Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J.,
RA   Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.;
RT   "Reductive genome evolution in Buchnera aphidicola.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000305};
CC   -!- SIMILARITY: Belongs to the IspH family. {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene. This sequence is shorter
CC       than orthologs in other Buchnera species. {ECO:0000305}.
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DR   EMBL; AE016826; AAO26871.1; -; Genomic_DNA.
DR   RefSeq; WP_011091272.1; NC_004545.1.
DR   AlphaFoldDB; Q89AV1; -.
DR   SMR; Q89AV1; -.
DR   STRING; 224915.bbp_137; -.
DR   EnsemblBacteria; AAO26871; AAO26871; bbp_137.
DR   GeneID; 56470681; -.
DR   KEGG; bab:bbp_137; -.
DR   eggNOG; COG0761; Bacteria.
DR   HOGENOM; CLU_027486_1_1_6; -.
DR   OMA; NDSICRQ; -.
DR   OrthoDB; 1311145at2; -.
DR   Proteomes; UP000000601; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0051745; F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0050992; P:dimethylallyl diphosphate biosynthetic process; IEA:InterPro.
DR   GO; GO:0019288; P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway; IEA:InterPro.
DR   InterPro; IPR003451; LytB/IspH.
DR   PANTHER; PTHR30426; PTHR30426; 1.
DR   Pfam; PF02401; LYTB; 1.
PE   5: Uncertain;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome.
FT   CHAIN           1..221
FT                   /note="Putative 4-hydroxy-3-methylbut-2-enyl diphosphate
FT                   reductase homolog"
FT                   /id="PRO_0000128906"
FT   BINDING         12
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250|UniProtKB:P62623"
FT   BINDING         96
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250|UniProtKB:P62623"
FT   BINDING         198
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250|UniProtKB:P62623"
SQ   SEQUENCE   221 AA;  25670 MW;  804A2BBF4D9A49C9 CRC64;
     MKILLENPRS FCVETKRALS IVNLALKTWG KLIYVNHKIV HNNYIINNLK LKGVIFSKNI
     NSILESSKLM LSKHSVPNKI KKTTLEKKIF IIDATCPLVK KVHKKIHYKN KNTYNVILIE
     NSQHPKIQGT LERYQKNLKN SIYIVKSKKY AIKLTIKNTR NSIITAQITL SSEKIYPIIT
     ILRKKFPAII NSHENNICYA TINRKTAIKH ISKKIRHYSC H