ID   ISM2_DANRE              Reviewed;         437 AA.
AC   Q58T08; Q1JQ38;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Isthmin-2;
DE   Flags: Precursor;
GN   Name=ism2;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Raible F., Araki I., Brand M.;
RT   "Isthmin-1 is regulated by Fgf and TGF beta signalling and contributes to
RT   early patterning of the zebrafish embryo.";
RL   Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-197.
RC   TISSUE=Larva;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the isthmin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAI16498.1; Type=Miscellaneous discrepancy; Note=Potential poly-A sequence.; Evidence={ECO:0000305};
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DR   EMBL; AY856372; AAX52520.1; -; mRNA.
DR   EMBL; BC116497; AAI16498.1; ALT_SEQ; mRNA.
DR   RefSeq; NP_001018345.1; NM_001020509.1.
DR   AlphaFoldDB; Q58T08; -.
DR   SMR; Q58T08; -.
DR   STRING; 7955.ENSDARP00000070453; -.
DR   PaxDb; Q58T08; -.
DR   PRIDE; Q58T08; -.
DR   GeneID; 553163; -.
DR   KEGG; dre:553163; -.
DR   CTD; 553163; -.
DR   ZFIN; ZDB-GENE-050523-4; ism2b.
DR   eggNOG; ENOG502RU1R; Eukaryota.
DR   InParanoid; Q58T08; -.
DR   OrthoDB; 876510at2759; -.
DR   PhylomeDB; Q58T08; -.
DR   PRO; PR:Q58T08; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.20.100.10; -; 1.
DR   InterPro; IPR005533; AMOP_dom.
DR   InterPro; IPR042413; ISM.
DR   InterPro; IPR000884; TSP1_rpt.
DR   InterPro; IPR036383; TSP1_rpt_sf.
DR   PANTHER; PTHR10239:SF28; PTHR10239:SF28; 1.
DR   Pfam; PF03782; AMOP; 1.
DR   Pfam; PF00090; TSP_1; 1.
DR   SMART; SM00723; AMOP; 1.
DR   SMART; SM00209; TSP1; 1.
DR   SUPFAM; SSF82895; SSF82895; 1.
DR   PROSITE; PS50856; AMOP; 1.
DR   PROSITE; PS50092; TSP1; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..437
FT                   /note="Isthmin-2"
FT                   /id="PRO_0000348259"
FT   DOMAIN          197..242
FT                   /note="TSP type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00210"
FT   DOMAIN          262..425
FT                   /note="AMOP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00347"
FT   REGION          156..191
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        160..176
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        217
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        258
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        349
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        209..236
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00210"
FT   DISULFID        213..241
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00210"
FT   DISULFID        224..228
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00210"
SQ   SEQUENCE   437 AA;  49717 MW;  29EE2B79EC4E0490 CRC64;
     MLRARKGLWV LLSVLLAFWI ERAISFPVRH HKRSRNGVYV ENQVQNHLAD SHPHQRRWLQ
     HHSTGVLPLP EPEEESKPFV LDFKNLPDLA NADIGSQNPN IQVTIEVLDD PPMDVEMDLV
     KEWSNDWSTS SPSSTVEWLG GKKLFWPLFW GYTDADSGED GTGQAEDEED DYDYDSGEPI
     PSGLGKTDGD WTHNRYEEKE EEWSTWSPCS VTCGHGNQTR SRSCGDFCTS TESQSCDLVP
     CPDDWNSVGH VFPFEMENGT EPYGTDVGSC EKWLNCKSEF LQRYLQQVFT ELPNCPCSYP
     SHVSNNIVSL LDVGHERSFQ WRDASGPKER LDIYKPSARS CLRSGLSKNG TTLAAQHCCY
     DDNKQLITRG KGAGTPNLIS TEFSPELHFK VDVLPWILCK GDWSRFHAVR PPNNGLHCME
     NPQQDIFMNE LEEAREY