ISL2_MOUSE
ID ISL2_MOUSE Reviewed; 359 AA.
AC Q9CXV0; G5E888;
DT 15-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Insulin gene enhancer protein ISL-2;
DE Short=Islet-2;
GN Name=Isl2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryonic head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.16 ANGSTROMS) OF 272-301 IN COMPLEX WITH LHX4.
RX PubMed=22025611; DOI=10.1074/jbc.m111.248559;
RA Gadd M.S., Bhati M., Jeffries C.M., Langley D.B., Trewhella J., Guss J.M.,
RA Matthews J.M.;
RT "Structural basis for partial redundancy in a class of transcription
RT factors, the LIM homeodomain proteins, in neural cell type specification.";
RL J. Biol. Chem. 286:42971-42980(2011).
CC -!- FUNCTION: Transcriptional factor that defines subclasses of motoneurons
CC that segregate into columns in the spinal cord and select distinct axon
CC pathways. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with LHX4. {ECO:0000269|PubMed:22025611}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
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DR EMBL; AK013964; BAB29088.1; -; mRNA.
DR EMBL; AC116699; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466522; EDL25854.1; -; Genomic_DNA.
DR CCDS; CCDS23205.1; -.
DR RefSeq; NP_081673.2; NM_027397.3.
DR PDB; 3MMK; X-ray; 2.16 A; A/B=272-301.
DR PDB; 6CME; X-ray; 1.92 A; A/B=153-159.
DR PDBsum; 3MMK; -.
DR PDBsum; 6CME; -.
DR AlphaFoldDB; Q9CXV0; -.
DR SASBDB; Q9CXV0; -.
DR SMR; Q9CXV0; -.
DR BioGRID; 222577; 8.
DR IntAct; Q9CXV0; 8.
DR STRING; 10090.ENSMUSP00000034869; -.
DR iPTMnet; Q9CXV0; -.
DR PhosphoSitePlus; Q9CXV0; -.
DR MaxQB; Q9CXV0; -.
DR PaxDb; Q9CXV0; -.
DR PRIDE; Q9CXV0; -.
DR ProteomicsDB; 269102; -.
DR Antibodypedia; 27431; 227 antibodies from 29 providers.
DR DNASU; 104360; -.
DR Ensembl; ENSMUST00000034869; ENSMUSP00000034869; ENSMUSG00000032318.
DR GeneID; 104360; -.
DR KEGG; mmu:104360; -.
DR UCSC; uc009psm.1; mouse.
DR CTD; 64843; -.
DR MGI; MGI:109156; Isl2.
DR VEuPathDB; HostDB:ENSMUSG00000032318; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000153731; -.
DR HOGENOM; CLU_027802_2_0_1; -.
DR InParanoid; Q9CXV0; -.
DR OMA; QMRDMAV; -.
DR OrthoDB; 948890at2759; -.
DR PhylomeDB; Q9CXV0; -.
DR TreeFam; TF315442; -.
DR BioGRID-ORCS; 104360; 1 hit in 74 CRISPR screens.
DR PRO; PR:Q9CXV0; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q9CXV0; protein.
DR Bgee; ENSMUSG00000032318; Expressed in lumbar dorsal root ganglion and 82 other tissues.
DR ExpressionAtlas; Q9CXV0; baseline and differential.
DR Genevisible; Q9CXV0; MM.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0007409; P:axonogenesis; IBA:GO_Central.
DR GO; GO:0045665; P:negative regulation of neuron differentiation; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; IMP:MGI.
DR GO; GO:0048663; P:neuron fate commitment; IMP:MGI.
DR GO; GO:0048665; P:neuron fate specification; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0031290; P:retinal ganglion cell axon guidance; IMP:MGI.
DR GO; GO:0021520; P:spinal cord motor neuron cell fate specification; IGI:MGI.
DR GO; GO:0021524; P:visceral motor neuron differentiation; IMP:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00132; LIM; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Developmental protein; DNA-binding; Homeobox; LIM domain;
KW Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Zinc.
FT CHAIN 1..359
FT /note="Insulin gene enhancer protein ISL-2"
FT /id="PRO_0000075753"
FT DOMAIN 25..86
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 87..149
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DNA_BIND 191..250
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 151..190
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 272..301
FT /note="LIM-binding domain (LID)"
FT REGION 326..359
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 154
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96A47"
FT MOD_RES 157
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96A47"
FT MOD_RES 279
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96A47"
FT CONFLICT 157
FT /note="S -> T (in Ref. 1; BAB29088)"
FT /evidence="ECO:0000305"
FT CONFLICT 185
FT /note="H -> D (in Ref. 1; BAB29088)"
FT /evidence="ECO:0000305"
FT CONFLICT 267
FT /note="L -> F (in Ref. 1; BAB29088)"
FT /evidence="ECO:0000305"
FT CONFLICT 282
FT /note="R -> G (in Ref. 1; BAB29088)"
FT /evidence="ECO:0000305"
FT STRAND 154..157
FT /evidence="ECO:0007829|PDB:6CME"
FT STRAND 160..164
FT /evidence="ECO:0007829|PDB:3MMK"
SQ SEQUENCE 359 AA; 39728 MW; A3745127E6C5FD61 CRC64;
MVDIIFHYPF LGAMGDHSKK KPGTAMCVGC GSQIHDQFIL RVSPDLEWHA ACLKCAECSQ
YLDETCTCFV RDGKTYCKRD YVRLFGIKCA QCQVGFSSSD LVMRARDSVY HIECFRCSVC
SRQLLPGDEF SLREHELLCR ADHGLLLERA AAGSPRSPGP LPGARGLHLP DAGSGRQPSL
RTHVHKQAEK TTRVRTVLNE KQLHTLRTCY AANPRPDALM KEQLVEMTGL SPRVIRVWFQ
NKRCKDKKKS ILMKQLQQQQ HSDKASLQGL TGTPLVAGSP IRHENAVQGS AVEVQTYQPP
WKALSEFALQ SDLDQPAFQQ LVSFSESGSL GNSSGSDVTS LSSQLPDTPN SMVPSPVET
