ID   ISL2B_ONCTS             Reviewed;         340 AA.
AC   P50212;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Insulin gene enhancer protein ISL-2B;
DE            Short=Islet-2B;
DE   Flags: Fragment;
GN   Name=isl2b;
OS   Oncorhynchus tshawytscha (Chinook salmon) (Salmo tshawytscha).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC   Salmonidae; Salmoninae; Oncorhynchus.
OX   NCBI_TaxID=74940;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Pituitary;
RX   PubMed=7852419; DOI=10.1074/jbc.270.7.3335;
RA   Gong Z., Hui C.-C., Hew C.-L.;
RT   "Presence of isl-1-related LIM domain homeobox genes in teleost and their
RT   similar patterns of expression in brain and spinal cord.";
RL   J. Biol. Chem. 270:3335-3345(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Pituitary;
RX   PubMed=7873614; DOI=10.1016/0167-4781(94)00228-u;
RA   Gong Z., Hew C.-L.;
RT   "Several splicing variants of isl-1 like genes in the chinook salmon
RT   (Oncorhynchus tschawytscha) encode truncated transcription factors
RT   containing a complete LIM domain.";
RL   Biochim. Biophys. Acta 1260:349-354(1995).
CC   -!- FUNCTION: Binds to one of the cis-acting domain of the insulin gene
CC       enhancer. May be involved in subtype specialization of primary
CC       motoneurons.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
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DR   EMBL; X64884; CAA46102.1; -; mRNA.
DR   PIR; B55973; B55973.
DR   AlphaFoldDB; P50212; -.
DR   SMR; P50212; -.
DR   Proteomes; UP000694402; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0048513; P:animal organ development; IEA:UniProt.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR001781; Znf_LIM.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00412; LIM; 2.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00132; LIM; 2.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR   PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; LIM domain; Metal-binding;
KW   Nucleus; Reference proteome; Repeat; Zinc.
FT   CHAIN           <1..340
FT                   /note="Insulin gene enhancer protein ISL-2B"
FT                   /id="PRO_0000075758"
FT   DOMAIN          9..62
FT                   /note="LIM zinc-binding 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DOMAIN          71..125
FT                   /note="LIM zinc-binding 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DNA_BIND        172..231
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          307..340
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_TER         1
SQ   SEQUENCE   340 AA;  38263 MW;  BBE58D1FE75B9BF9 CRC64;
     KKKSGIAMCV GCGSQIHDQY ILRVAPDLEW HAACLKCSEC SQYLDETCTC FVRDGKTYCK
     RDYVRLFGIK CAKCNIGFCS SDLVMRARDN VYHMECFRCS VCSRQLVPGD EFSLRDEELL
     CRADHGLLLE RASAGSPISP GNILSRSFHI ADPVSVRQPP HRNHVHKQSE KTTRVRTVLN
     EKQLHTLRTC YNANPRPDAL MKEQLVEMTG LSPRVIRVWF QNKRCKDKKR TIFMKQLQQQ
     HHIDKTNLQG LTGTPMVAGS PIRHDNTVLG NPVEVQTYQP PWKALSEFAL QSDLDQPAFR
     QLVSFSESGS MGNSSGSDVT SLSSQLPDTP NSMVPSPMDT