ISL2B_DANRE
ID ISL2B_DANRE Reviewed; 358 AA.
AC P53407; Q504I1; Q6LD05;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Insulin gene enhancer protein isl-2b;
DE Short=Islet-2B;
DE AltName: Full=Insulin gene enhancer protein isl-3;
DE Short=Islet-3;
GN Name=isl2b; Synonyms=isl-3, isl3; ORFNames=zgc:109840;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=7556938; DOI=10.1006/dbio.1995.1306;
RA Tokumoto M., Gong Z., Tsubokawa T., Hew C.-L., Uyemura K., Hotta Y.,
RA Okamoto H.;
RT "Molecular heterogeneity among primary motoneurons and within myotomes
RT revealed by the differential mRNA expression of novel islet-1 homologs in
RT embryonic zebrafish.";
RL Dev. Biol. 171:578-589(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 71-237.
RC TISSUE=Embryo;
RX PubMed=7852419; DOI=10.1074/jbc.270.7.3335;
RA Gong Z., Hui C.-C., Hew C.-L.;
RT "Presence of isl-1-related LIM domain homeobox genes in teleost and their
RT similar patterns of expression in brain and spinal cord.";
RL J. Biol. Chem. 270:3335-3345(1995).
CC -!- FUNCTION: Binds to one of the cis-acting domain of the insulin gene
CC enhancer. May be involved in the regional specification of the myotome
CC and also in target recognition by the caudal primary neuron.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically. At 17
CC hours post-fertilization (hpf), expressed in dorsal Rohan-Beard neurons
CC and strongly in the ventral myotomes. At 24 hpf, head expression is
CC restricted to eyes, the tectal region of the midbrain, trigeminal
CC ganglia and the ganglia anterior to the otic vesicles.
CC {ECO:0000269|PubMed:7556938}.
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DR EMBL; D38454; BAA07485.1; -; mRNA.
DR EMBL; AL845510; CAI11499.1; -; Genomic_DNA.
DR EMBL; BC095011; AAH95011.1; -; mRNA.
DR EMBL; U09404; AAA80275.1; -; mRNA.
DR PIR; I51735; I51735.
DR RefSeq; NP_571039.1; NM_130964.2.
DR AlphaFoldDB; P53407; -.
DR BMRB; P53407; -.
DR SMR; P53407; -.
DR STRING; 7955.ENSDARP00000055935; -.
DR PaxDb; P53407; -.
DR Ensembl; ENSDART00000055936; ENSDARP00000055935; ENSDARG00000053499.
DR GeneID; 30151; -.
DR KEGG; dre:30151; -.
DR CTD; 30151; -.
DR ZFIN; ZDB-GENE-990415-133; isl2b.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000153731; -.
DR HOGENOM; CLU_027802_2_0_1; -.
DR InParanoid; P53407; -.
DR OMA; YQPPWKS; -.
DR OrthoDB; 948890at2759; -.
DR PhylomeDB; P53407; -.
DR TreeFam; TF315442; -.
DR PRO; PR:P53407; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 7.
DR Bgee; ENSDARG00000053499; Expressed in camera-type eye and 25 other tissues.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007409; P:axonogenesis; IBA:GO_Central.
DR GO; GO:0031017; P:exocrine pancreas development; IGI:ZFIN.
DR GO; GO:0048665; P:neuron fate specification; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0003139; P:secondary heart field specification; IMP:ZFIN.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00132; LIM; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; LIM domain; Metal-binding;
KW Nucleus; Reference proteome; Repeat; Zinc.
FT CHAIN 1..358
FT /note="Insulin gene enhancer protein isl-2b"
FT /id="PRO_0000075759"
FT DOMAIN 27..80
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 89..143
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DNA_BIND 191..250
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 325..358
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 358 AA; 39848 MW; 3D72568172411D67 CRC64;
MVDIIFSSSF LDDMGDHSKK KSGLAMCVGC GSQIHDQYIL RVSPDLEWHA ACLKCVECNQ
YLDETCTCFV RDGKTYCKRD YVRLFGIKCA KCTLGFSSSD LVMRARDSVY HIECFRCSVC
SRQLLPGDEF SVRDEELLCR ADHGLALERG PGGSPLSPGN IHTRGLHMAA DPVSVRQTPH
RNHVHKQSEK TTRVRTVLNE KQLHTLRTCY NANPRPDALM KEQLVEMTGL SPRVIRVWFQ
NKRCKDKKRS ILMKQLQQQH GDKTNLQGMT GTALVAGSPI RHNPSVPGHP VDVQAYQPPW
KALSEFALQS DLDQPAFQQL VSFSESGSLG NSSGSDVTSL SSQLPDTPNS MVPSPVET
