ISL2A_DANRE
ID ISL2A_DANRE Reviewed; 359 AA.
AC P53406;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Insulin gene enhancer protein isl-2a;
DE Short=Islet-2A;
DE AltName: Full=Insulin gene enhancer protein isl-2;
DE Short=Islet-2;
GN Name=isl2a; Synonyms=isl-2, isl2;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=7556938; DOI=10.1006/dbio.1995.1306;
RA Tokumoto M., Gong Z., Tsubokawa T., Hew C.-L., Uyemura K., Hotta Y.,
RA Okamoto H.;
RT "Molecular heterogeneity among primary motoneurons and within myotomes
RT revealed by the differential mRNA expression of novel islet-1 homologs in
RT embryonic zebrafish.";
RL Dev. Biol. 171:578-589(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8575312; DOI=10.1242/dev.121.12.4117;
RA Appel B., Korzh V., Glasgow E., Thor S., Edlund T., Dawid I.B., Eisen J.S.;
RT "Motoneuron fate specification revealed by patterned LIM homeobox gene
RT expression in embryonic zebrafish.";
RL Development 121:4117-4125(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 71-237.
RC TISSUE=Embryo;
RX PubMed=7852419; DOI=10.1074/jbc.270.7.3335;
RA Gong Z., Hui C.-C., Hew C.-L.;
RT "Presence of isl-1-related LIM domain homeobox genes in teleost and their
RT similar patterns of expression in brain and spinal cord.";
RL J. Biol. Chem. 270:3335-3345(1995).
CC -!- FUNCTION: Binds to one of the cis-acting domain of the insulin gene
CC enhancer. May be involved in subtype specialization of primary
CC motoneurons.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: First expressed at 15 hours post-fertilization
CC (hpf), segmentally in the ventral region of the spinal cord (primary
CC motoneurons) and then later in the dorsal regions (Rohan-Beard
CC neurons). At 18 hpf, also expressed in tail bud and pronephric duct. At
CC 24 hpf, expressed strongly in trigeminal ganglia. At 30 hpf, expressed
CC transiently in pectoral fin bud. {ECO:0000269|PubMed:7556938}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D38453; BAA07484.1; -; mRNA.
DR EMBL; X88805; CAA61283.1; -; mRNA.
DR EMBL; U09403; AAA80274.1; -; mRNA.
DR PIR; I51734; I51734.
DR RefSeq; NP_571045.1; NM_130970.1.
DR AlphaFoldDB; P53406; -.
DR SMR; P53406; -.
DR STRING; 7955.ENSDARP00000020636; -.
DR PaxDb; P53406; -.
DR Ensembl; ENSDART00000012862; ENSDARP00000020636; ENSDARG00000003971.
DR GeneID; 30157; -.
DR KEGG; dre:30157; -.
DR CTD; 30157; -.
DR ZFIN; ZDB-GENE-980526-562; isl2a.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000153731; -.
DR HOGENOM; CLU_027802_2_0_1; -.
DR InParanoid; P53406; -.
DR OMA; QMRDMAV; -.
DR OrthoDB; 948890at2759; -.
DR PhylomeDB; P53406; -.
DR TreeFam; TF315442; -.
DR PRO; PR:P53406; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 25.
DR Bgee; ENSDARG00000003971; Expressed in neuron and 48 other tissues.
DR ExpressionAtlas; P53406; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0035476; P:angioblast cell migration; IMP:ZFIN.
DR GO; GO:0048675; P:axon extension; IMP:ZFIN.
DR GO; GO:0007411; P:axon guidance; IMP:ZFIN.
DR GO; GO:0007409; P:axonogenesis; IMP:ZFIN.
DR GO; GO:0031017; P:exocrine pancreas development; IGI:ZFIN.
DR GO; GO:0048665; P:neuron fate specification; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048845; P:venous blood vessel morphogenesis; IMP:ZFIN.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00132; LIM; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; LIM domain; Metal-binding;
KW Nucleus; Reference proteome; Repeat; Zinc.
FT CHAIN 1..359
FT /note="Insulin gene enhancer protein isl-2a"
FT /id="PRO_0000075756"
FT DOMAIN 27..80
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 30..143
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DNA_BIND 191..250
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 171..190
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 326..359
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 359 AA; 40227 MW; AEAD810D2841BB37 CRC64;
MVDILPHPSF LGDMGDHSKK KSGIAMCVGC GSQIHDQYIL RVSPDLEWHA ACLKCAECSQ
YLDETCTCFV RDGKTYCKRD YVRLFGIKCA KCNIGFCSSD LVMRARDNVY HMECFRCSVC
SRHLLPGDEF SLRDEELLCR ADHGLLMERA SAGSPISPGN IHSSRPLHIP EPVPVRQPPH
RNHVHKQSEK TTRVRTVLNE KQLHTLRTCY NANPRPDALM KEQLVEMTGL SPRVIRVWFQ
NKRCKDKKKS ILMKQLQQQQ HNDKTNLQGL TGTPLVAGSP IRHDTTVQGN PVEVQTYQPP
WKALSEFALQ SDLDQPAFQQ LVSFSESGSL GNSSGSDVTS LSSQLPDTPN SMVPSPVET
