ISL1_DANRE
ID ISL1_DANRE Reviewed; 349 AA.
AC P53405; A2AWM5;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Insulin gene enhancer protein isl-1;
DE Short=Islet-1;
GN Name=isl1; Synonyms=isl-1; ORFNames=si:ch211-219f7.1;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX PubMed=8167375; DOI=10.1002/aja.1001990102;
RA Inoue A., Takahashi M., Hatta K., Hotta Y., Okamoto H.;
RT "Developmental regulation of islet-1 mRNA expression during neuronal
RT differentiation in embryonic zebrafish.";
RL Dev. Dyn. 199:1-11(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-binding transcriptional activator. Recognizes and binds
CC to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of
CC target genes. Plays a fundamental role in the gene regulatory network
CC essential for retinal ganglion cell (RGC) differentiation. May be
CC involved in subtype specialization of primary motoneurons. May bind to
CC insulin gene enhancer sequences. Essential for heart development (By
CC similarity). {ECO:0000250|UniProtKB:P61372,
CC ECO:0000250|UniProtKB:P61374}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P61372}.
CC -!- DEVELOPMENTAL STAGE: Expressed immediately after gastrulation in the
CC polster, cranial ganglia, rohan-beard neurons and in ventromedial cells
CC of the spinal cord at 12 hours post-fertilization (hpf). Expressed in
CC cells slightly anterior to the segment border at 16 hpf.
CC {ECO:0000269|PubMed:8167375}.
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DR EMBL; D21135; BAA04670.1; -; mRNA.
DR EMBL; AL954694; CAM14069.1; -; Genomic_DNA.
DR EMBL; BC060892; AAH60892.1; -; mRNA.
DR PIR; I51739; I51739.
DR RefSeq; NP_571037.1; NM_130962.1.
DR AlphaFoldDB; P53405; -.
DR BMRB; P53405; -.
DR SMR; P53405; -.
DR STRING; 7955.ENSDARP00000012903; -.
DR PaxDb; P53405; -.
DR Ensembl; ENSDART00000010896; ENSDARP00000012903; ENSDARG00000004023.
DR GeneID; 30147; -.
DR KEGG; dre:30147; -.
DR CTD; 30147; -.
DR ZFIN; ZDB-GENE-980526-112; isl1a.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000153731; -.
DR HOGENOM; CLU_027802_2_0_1; -.
DR InParanoid; P53405; -.
DR OMA; VSQMHGY; -.
DR OrthoDB; 948890at2759; -.
DR PhylomeDB; P53405; -.
DR TreeFam; TF315442; -.
DR PRO; PR:P53405; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 5.
DR Bgee; ENSDARG00000004023; Expressed in neuron and 121 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:ZFIN.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0055011; P:atrial cardiac muscle cell differentiation; IMP:ZFIN.
DR GO; GO:0048675; P:axon extension; IMP:ZFIN.
DR GO; GO:0007409; P:axonogenesis; IBA:GO_Central.
DR GO; GO:0031018; P:endocrine pancreas development; IMP:ZFIN.
DR GO; GO:0031017; P:exocrine pancreas development; IMP:ZFIN.
DR GO; GO:0097154; P:GABAergic neuron differentiation; IMP:ZFIN.
DR GO; GO:0007507; P:heart development; ISS:UniProtKB.
DR GO; GO:0048666; P:neuron development; IMP:ZFIN.
DR GO; GO:0048665; P:neuron fate specification; IMP:ZFIN.
DR GO; GO:0003322; P:pancreatic A cell development; IMP:ZFIN.
DR GO; GO:0003324; P:pancreatic D cell development; IMP:ZFIN.
DR GO; GO:0048936; P:peripheral nervous system neuron axonogenesis; IMP:ZFIN.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0008016; P:regulation of heart contraction; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0060931; P:sinoatrial node cell development; IMP:BHF-UCL.
DR GO; GO:0021527; P:spinal cord association neuron differentiation; IMP:ZFIN.
DR GO; GO:0021522; P:spinal cord motor neuron differentiation; IMP:ZFIN.
DR GO; GO:0003323; P:type B pancreatic cell development; IMP:ZFIN.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00132; LIM; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; Differentiation; DNA-binding; Homeobox;
KW LIM domain; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc.
FT CHAIN 1..349
FT /note="Insulin gene enhancer protein isl-1"
FT /id="PRO_0000075751"
FT DOMAIN 17..70
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 79..133
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DNA_BIND 181..240
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 312..349
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 312..341
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 349 AA; 39181 MW; F6F898870B4E378E CRC64;
MGDMGDPPKK KRLISLCVGC GNQIHDQYIL RVSPDLEWHA ACLKCAECNQ YLDESCTCFV
RDGKTYCKRD YIRLYGIKCA KCNIGFSKND FVMRARSKVY HIECFRCVAC SRQLIPGDEF
ALREDGLFCR ADHDVVERAT MGAGDPLSPL HPARPLQMAA EPISARQPAL RPHVHKQPEK
TTRVRTVLNE KQLHTLRTCY NANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKRS
ILMKQLQQQQ PNDKTNIQGM TGTPMVATSP ERHDGGLQAN QVEVQSYQPP WKVLSDFALQ
SDIDQPAFQQ LVNFSEGGPG SNSTGSEVAS MSSQLPDTPN SMVASPIEA
