ISL1_CHICK
ID ISL1_CHICK Reviewed; 349 AA.
AC P50211;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Insulin gene enhancer protein ISL-1;
DE Short=Islet-1 {ECO:0000303|PubMed:7528105};
GN Name=ISL1; Synonyms=ISL-1;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RC TISSUE=Spinal cord;
RX PubMed=7528105; DOI=10.1016/0092-8674(94)90027-2;
RA Tsuchida T., Ensini M., Morton S.B., Baldassare M., Edlund T.,
RA Jessell T.M., Pfaff S.L.;
RT "Topographic organization of embryonic motor neurons defined by expression
RT of LIM homeobox genes.";
RL Cell 79:957-970(1994).
CC -!- FUNCTION: Acts as a transcriptional regulator (PubMed:7528105).
CC Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-
CC 3' in promoter of target genes. Plays a fundamental role in the gene
CC regulatory network essential for retinal ganglion cell (RGC)
CC differentiation. Binds to insulin gene enhancer sequences (By
CC similarity). Defines subclasses of motoneurons that segregate into
CC columns in the spinal cord and select distinct axon pathways
CC (PubMed:7528105). Acts in conjunction with LHX1, LHX3 and ISL2
CC (PubMed:7528105). Binds to insulin gene enhancer sequences (By
CC similarity). Essential for heart development (By similarity).
CC {ECO:0000250|UniProtKB:P61372, ECO:0000250|UniProtKB:P61374,
CC ECO:0000269|PubMed:7528105}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P61372}.
CC -!- DEVELOPMENTAL STAGE: Expressed in motor neurons between stages 34 and
CC 35 (at protein level). Expressed prior to the formation of distinct
CC motor axon pathways and before the segregation of motor neurons into
CC columns. Expressed throughout the median motor column and the medial
CC subdivision of the lateral motor column (LMCm).
CC {ECO:0000269|PubMed:7528105}.
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DR EMBL; L35567; AAA62171.1; -; mRNA.
DR PIR; I50369; I50369.
DR RefSeq; NP_990745.1; NM_205414.1.
DR AlphaFoldDB; P50211; -.
DR BMRB; P50211; -.
DR SMR; P50211; -.
DR STRING; 9031.ENSGALP00000043151; -.
DR Ensembl; ENSGALT00000044538; ENSGALP00000043151; ENSGALG00000014884.
DR GeneID; 396383; -.
DR KEGG; gga:396383; -.
DR CTD; 3670; -.
DR VEuPathDB; HostDB:geneid_396383; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000153731; -.
DR HOGENOM; CLU_027802_2_0_1; -.
DR InParanoid; P50211; -.
DR OMA; VSQMHGY; -.
DR OrthoDB; 948890at2759; -.
DR PhylomeDB; P50211; -.
DR PRO; PR:P50211; -.
DR Proteomes; UP000000539; Chromosome Z.
DR Bgee; ENSGALG00000014884; Expressed in brain.
DR GO; GO:0005634; C:nucleus; IDA:AgBase.
DR GO; GO:0043425; F:bHLH transcription factor binding; IEA:Ensembl.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:Ensembl.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:1990841; F:promoter-specific chromatin binding; IEA:Ensembl.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IEA:Ensembl.
DR GO; GO:0060413; P:atrial septum morphogenesis; IEA:Ensembl.
DR GO; GO:0007409; P:axonogenesis; IBA:GO_Central.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; IEA:Ensembl.
DR GO; GO:0060913; P:cardiac cell fate determination; IEA:Ensembl.
DR GO; GO:0060379; P:cardiac muscle cell myoblast differentiation; IEA:Ensembl.
DR GO; GO:0003215; P:cardiac right ventricle morphogenesis; IEA:Ensembl.
DR GO; GO:0008283; P:cell population proliferation; IEA:Ensembl.
DR GO; GO:0003203; P:endocardial cushion morphogenesis; IEA:Ensembl.
DR GO; GO:0007507; P:heart development; ISS:UniProtKB.
DR GO; GO:0060384; P:innervation; IEA:Ensembl.
DR GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IEA:Ensembl.
DR GO; GO:0050728; P:negative regulation of inflammatory response; IEA:Ensembl.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Ensembl.
DR GO; GO:0045665; P:negative regulation of neuron differentiation; IEA:Ensembl.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0001755; P:neural crest cell migration; IEA:Ensembl.
DR GO; GO:0048665; P:neuron fate specification; IBA:GO_Central.
DR GO; GO:0003148; P:outflow tract septum morphogenesis; IEA:Ensembl.
DR GO; GO:0031016; P:pancreas development; IEA:Ensembl.
DR GO; GO:0048936; P:peripheral nervous system neuron axonogenesis; IEA:Ensembl.
DR GO; GO:0060037; P:pharyngeal system development; IEA:Ensembl.
DR GO; GO:0021983; P:pituitary gland development; IEA:Ensembl.
DR GO; GO:0045766; P:positive regulation of angiogenesis; IEA:Ensembl.
DR GO; GO:0045597; P:positive regulation of cell differentiation; IEA:Ensembl.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IEA:Ensembl.
DR GO; GO:0043388; P:positive regulation of DNA binding; IEA:Ensembl.
DR GO; GO:0071657; P:positive regulation of granulocyte colony-stimulating factor production; IEA:Ensembl.
DR GO; GO:0032725; P:positive regulation of granulocyte macrophage colony-stimulating factor production; IEA:Ensembl.
DR GO; GO:0035066; P:positive regulation of histone acetylation; IEA:Ensembl.
DR GO; GO:0032729; P:positive regulation of interferon-gamma production; IEA:Ensembl.
DR GO; GO:0032730; P:positive regulation of interleukin-1 alpha production; IEA:Ensembl.
DR GO; GO:0032731; P:positive regulation of interleukin-1 beta production; IEA:Ensembl.
DR GO; GO:0032735; P:positive regulation of interleukin-12 production; IEA:Ensembl.
DR GO; GO:0032755; P:positive regulation of interleukin-6 production; IEA:Ensembl.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0032760; P:positive regulation of tumor necrosis factor production; IEA:Ensembl.
DR GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; IEA:Ensembl.
DR GO; GO:0010575; P:positive regulation of vascular endothelial growth factor production; IEA:Ensembl.
DR GO; GO:0086091; P:regulation of heart rate by cardiac conduction; IEA:Ensembl.
DR GO; GO:0003266; P:regulation of secondary heart field cardioblast proliferation; IEA:Ensembl.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0031290; P:retinal ganglion cell axon guidance; IEA:Ensembl.
DR GO; GO:0003139; P:secondary heart field specification; IEA:Ensembl.
DR GO; GO:0048880; P:sensory system development; IEA:Ensembl.
DR GO; GO:0060931; P:sinoatrial node cell development; IEA:Ensembl.
DR GO; GO:0021520; P:spinal cord motor neuron cell fate specification; IEA:Ensembl.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IEA:Ensembl.
DR GO; GO:0021559; P:trigeminal nerve development; IEA:Ensembl.
DR GO; GO:0055010; P:ventricular cardiac muscle tissue morphogenesis; IEA:Ensembl.
DR GO; GO:0021524; P:visceral motor neuron differentiation; IEA:Ensembl.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00132; LIM; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Differentiation; DNA-binding; Homeobox;
KW LIM domain; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc.
FT CHAIN 1..349
FT /note="Insulin gene enhancer protein ISL-1"
FT /id="PRO_0000075750"
FT DOMAIN 17..70
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 79..133
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DNA_BIND 181..240
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 312..349
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 312..341
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 349 AA; 39034 MW; 286E2B364EE0A505 CRC64;
MGDMGDPPKK KRLISLCVGC GNQIHDQYIL RVSPDLEWHA ACLKCAECNQ YLDETCTCFV
RDGKTYCKRD YIRLYGIKCA KCSIGFSKND FVMRARAKVY HIECFRCVAC SRQLIPGDEF
ALREDGLFCR ADHDVVERAS LGAGDPLSPL HPARPLQMAA EPISARQPAL RPHVHKQPEK
TTRVRTVLNE KQLHTLRTCY AANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKRS
IMMKQLQQQQ PNDKTNIQGM TGTPMVAASP ERHDGGLQAN PVEVQSYQPP WKVLSDFALQ
SDIDQPAFQQ LVNFSEGGPG SNSTGSEVAS MSSQLPDTPN SMVASPIEA
