ID   ISCU_AZOVI              Reviewed;         128 AA.
AC   O31270;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Iron-sulfur cluster assembly scaffold protein IscU;
GN   Name=iscU;
OS   Azotobacter vinelandii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 13705 / OP1 / DSM 366 / NCIMB 11614 / LMG 3878 / UW;
RX   PubMed=9582371; DOI=10.1074/jbc.273.21.13264;
RA   Zheng L., Cash V.L., Flint D.H., Dean D.R.;
RT   "Assembly of iron-sulfur clusters. Identification of an iscSUA-hscBA-fdx
RT   gene cluster from Azotobacter vinelandii.";
RL   J. Biol. Chem. 273:13264-13272(1998).
RN   [2]
RP   FUNCTION, FE-S CLUSTER-BINDING, AND INTERACTION WITH ISCS.
RC   STRAIN=ATCC 13705 / OP1 / DSM 366 / NCIMB 11614 / LMG 3878 / UW;
RX   DOI=10.1021/ja9944195;
RA   Agar J.N., Zheng L., Cash V.L., Dean D.R., Johnson M.K.;
RT   "Role of the iscU protein in iron-sulfur cluster biosynthesis: IscS-
RT   mediated assembly of a [Fe2S2] cluster in iscU.";
RL   J. Am. Chem. Soc. 122:2136-2137(2000).
RN   [3]
RP   FUNCTION, AND FORMATION OF FE-S CLUSTERS.
RC   STRAIN=ATCC 13705 / OP1 / DSM 366 / NCIMB 11614 / LMG 3878 / UW;
RX   PubMed=10891064; DOI=10.1021/bi000931n;
RA   Agar J.N., Krebs C., Frazzon J., Huynh B.H., Dean D.R., Johnson M.K.;
RT   "IscU as a scaffold for iron-sulfur cluster biosynthesis: sequential
RT   assembly of [2Fe-2S] and [4Fe-4S] clusters in IscU.";
RL   Biochemistry 39:7856-7862(2000).
CC   -!- FUNCTION: A scaffold on which IscS assembles Fe-S clusters. Cluster
CC       assembly on IscU homodimers proceeds sequentially from 1 2Fe-2S per
CC       dimer, to 2 2Fe-2S per dimer and finally 1 4Fe-4S per dimer.
CC       Subsequently IscU transfers the Fe-S cluster to other acceptor
CC       proteins. It is likely that Fe-S cluster coordination is flexible as
CC       the role of this complex is to build and then hand off Fe-S clusters.
CC       The clusters are very sensitive to O(2). {ECO:0000269|PubMed:10891064,
CC       ECO:0000269|Ref.2}.
CC   -!- SUBUNIT: Homodimer. Forms a heterotetramer with IscS; each subunit of
CC       the IscS dimer contacts an IscU monomer.
CC   -!- INTERACTION:
CC       O31270; O31269: iscS; NbExp=3; IntAct=EBI-15710405, EBI-15710417;
CC   -!- SIMILARITY: Belongs to the NifU family. {ECO:0000305}.
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DR   EMBL; AF010139; AAC24473.1; -; Genomic_DNA.
DR   PIR; T44282; T44282.
DR   RefSeq; WP_012702550.1; NZ_FPKM01000003.1.
DR   AlphaFoldDB; O31270; -.
DR   SMR; O31270; -.
DR   DIP; DIP-46128N; -.
DR   IntAct; O31270; 1.
DR   OMA; YKNPRNY; -.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR   CDD; cd06664; IscU_like; 1.
DR   InterPro; IPR011339; ISC_FeS_clus_asmbl_IscU.
DR   InterPro; IPR002871; NIF_FeS_clus_asmbl_NifU_N.
DR   PANTHER; PTHR10093; PTHR10093; 1.
DR   Pfam; PF01592; NifU_N; 1.
DR   TIGRFAMs; TIGR01999; iscU; 1.
PE   1: Evidence at protein level;
FT   CHAIN           1..128
FT                   /note="Iron-sulfur cluster assembly scaffold protein IscU"
FT                   /id="PRO_0000428752"
SQ   SEQUENCE   128 AA;  13875 MW;  026073E77A3744C0 CRC64;
     MAYSDKVIDH YENPRNVGKL DAQDPDVGTG MVGAPACGDV MRLQIKVNEQ GIIEDAKFKT
     YGCGSAIASS SLATEWMKGR TLEEAETIKN TQIAEELALP PVKIHCSVLA EDAIKAAVRD
     YKHKKGLV