ID   IRAD_ECO5E              Reviewed;         130 AA.
AC   B5Z3R6;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Anti-adapter protein IraD {ECO:0000255|HAMAP-Rule:MF_02010};
GN   Name=iraD {ECO:0000255|HAMAP-Rule:MF_02010};
GN   OrderedLocusNames=ECH74115_5835;
OS   Escherichia coli O157:H7 (strain EC4115 / EHEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=444450;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EC4115 / EHEC;
RX   PubMed=22135463; DOI=10.1073/pnas.1107176108;
RA   Eppinger M., Mammel M.K., Leclerc J.E., Ravel J., Cebula T.A.;
RT   "Genomic anatomy of Escherichia coli O157:H7 outbreaks.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:20142-20147(2011).
CC   -!- FUNCTION: Inhibits RpoS proteolysis by regulating RssB activity,
CC       thereby increasing the stability of the sigma stress factor RpoS during
CC       oxidative stress. Its effect on RpoS stability is due to its
CC       interaction with RssB, which probably blocks the interaction of RssB
CC       with RpoS, and the consequent delivery of the RssB-RpoS complex to the
CC       ClpXP protein degradation pathway. {ECO:0000255|HAMAP-Rule:MF_02010}.
CC   -!- SUBUNIT: Interacts with RssB. {ECO:0000255|HAMAP-Rule:MF_02010}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02010}.
CC   -!- SIMILARITY: Belongs to the GpW/Gp25 family. IraD subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_02010}.
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DR   EMBL; CP001164; ACI35810.1; -; Genomic_DNA.
DR   RefSeq; WP_001301619.1; NC_011353.1.
DR   AlphaFoldDB; B5Z3R6; -.
DR   SMR; B5Z3R6; -.
DR   KEGG; ecf:ECH74115_5835; -.
DR   HOGENOM; CLU_1977621_0_0_6; -.
DR   OMA; FKEAYCH; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0043856; F:anti-sigma factor antagonist activity; IEA:InterPro.
DR   GO; GO:0006974; P:cellular response to DNA damage stimulus; IEA:InterPro.
DR   GO; GO:0034599; P:cellular response to oxidative stress; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_02010; IraD; 1.
DR   InterPro; IPR023776; Anti-adapt_IraD.
DR   InterPro; IPR007048; IraD/Gp25-like.
DR   Pfam; PF04965; GPW_gp25; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Stress response.
FT   CHAIN           1..130
FT                   /note="Anti-adapter protein IraD"
FT                   /id="PRO_1000189480"
SQ   SEQUENCE   130 AA;  14803 MW;  617779FEB037C5E4 CRC64;
     MMRQSLQAVL PEISGNKTSL LRKSVCSDLL TLFNSPHSTL PSLLVSGMPE WQVHNPSDKH
     LQSWYCRQLR SALLFHEPRI AALQVNLKEA YCHTLAISLE IMLYHDDEPL TFDLVWDNGG
     WRSATLENVS