ID   INSI1_ECOLI             Reviewed;         383 AA.
AC   P0CF88; P37246; P77341; Q2M630; Q2MCF8;
DT   18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Transposase InsI for insertion sequence element IS30A;
GN   Name=insI1; OrderedLocusNames=b0256, JW0246;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA   Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA   Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA   Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT   - 6.0 min (189,987 - 281,416bp) region.";
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Required for the transposition of the insertion element.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the transposase IS30 family. {ECO:0000305}.
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DR   EMBL; U70214; AAB08675.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73359.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76048.1; -; Genomic_DNA.
DR   RefSeq; NP_414790.1; NC_000913.3.
DR   RefSeq; WP_001254938.1; NZ_LN832404.1.
DR   AlphaFoldDB; P0CF88; -.
DR   STRING; 511145.b0256; -.
DR   PaxDb; P0CF88; -.
DR   PRIDE; P0CF88; -.
DR   EnsemblBacteria; AAC73359; AAC73359; b0256.
DR   EnsemblBacteria; BAE76048; BAE76048; BAE76048.
DR   GeneID; 949005; -.
DR   KEGG; ecj:JW0246; -.
DR   KEGG; eco:b0256; -.
DR   PATRIC; fig|511145.12.peg.259; -.
DR   EchoBASE; EB4703; -.
DR   eggNOG; COG2826; Bacteria.
DR   HOGENOM; CLU_035706_0_0_6; -.
DR   InParanoid; P0CF88; -.
DR   OMA; RKTITWD; -.
DR   PhylomeDB; P0CF88; -.
DR   BioCyc; EcoCyc:G6131-MON; -.
DR   BioCyc; MetaCyc:G6131-MON; -.
DR   PRO; PR:P0CF88; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0032993; C:protein-DNA complex; IDA:EcoCyc.
DR   GO; GO:0032135; F:DNA insertion or deletion binding; IDA:EcoCyc.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:EcoCyc.
DR   GO; GO:0004803; F:transposase activity; IBA:GO_Central.
DR   GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR   GO; GO:0032196; P:transposition; IBA:GO_Central.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IDA:EcoCyc.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR001584; Integrase_cat-core.
DR   InterPro; IPR025246; IS30-like_HTH.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR001598; Transposase_IS30_CS.
DR   Pfam; PF13936; HTH_38; 1.
DR   Pfam; PF00665; rve; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50994; INTEGRASE; 1.
DR   PROSITE; PS01043; TRANSPOSASE_IS30; 1.
PE   3: Inferred from homology;
KW   DNA recombination; DNA-binding; Reference proteome; Transposable element;
KW   Transposition.
FT   CHAIN           1..383
FT                   /note="Transposase InsI for insertion sequence element
FT                   IS30A"
FT                   /id="PRO_0000211896"
FT   DOMAIN          213..379
FT                   /note="Integrase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
SQ   SEQUENCE   383 AA;  44267 MW;  7F4DFD2D7A4B0F83 CRC64;
     MRRTFTAEEK ASVFELWKNG TGFSEIANIL GSKPGTIFTM LRDTGGIKPH ERKRAVAHLT
     LSEREEIRAG LSAKMSIRAI ATALNRSPST ISREVQRNRG RRYYKAVDAN NRANRMAKRP
     KPCLLDQNLP LRKLVLEKLE MKWSPEQISG WLRRTKPRQK TLRISPETIY KTLYFRSREA
     LHHLNIQHLR RSHSLRHGRR HTRKGERGTI NIVNGTPIHE RSRNIDNRRS LGHWEGDLVS
     GTKNSHIATL VDRKSRYTII VRLRGKDSVS VNQALTDKFL SLPSELRKSL TWDRGMELAR
     HLEFTVSTGV KVYFCDPQSP WQRGTNENTN GLIRQYFPKK TCLAQYTQHE LDLVAAQLNN
     RPRKTLKFKT PKEIIERGVA LTD