ID   INSG_ECOLI              Reviewed;         442 AA.
AC   P03835; Q2M634;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Transposase InsG for insertion sequence element IS4;
GN   Name=insG; OrderedLocusNames=b4278, JW5767;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=6268937; DOI=10.1007/bf00268423;
RA   Klaer R., Kuhn S., Tillmann E., Fritz H.-J., Starlinger P.;
RT   "The sequence of IS4.";
RL   Mol. Gen. Genet. 181:169-175(1981).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA   Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT   from 92.8 through 100 minutes.";
RL   Nucleic Acids Res. 23:2105-2119(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Involved in the transposition of the insertion sequence IS4.
CC   -!- SIMILARITY: Belongs to the transposase 11 family. {ECO:0000305}.
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DR   EMBL; J01733; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; U14003; AAA97174.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC77234.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE78272.1; -; Genomic_DNA.
DR   PIR; A04463; IEEC41.
DR   RefSeq; NP_418698.1; NC_000913.3.
DR   RefSeq; WP_000547191.1; NZ_CP047127.1.
DR   AlphaFoldDB; P03835; -.
DR   STRING; 511145.b4278; -.
DR   PaxDb; P03835; -.
DR   PRIDE; P03835; -.
DR   EnsemblBacteria; AAC77234; AAC77234; b4278.
DR   EnsemblBacteria; BAE78272; BAE78272; BAE78272.
DR   GeneID; 948805; -.
DR   KEGG; ecj:JW5767; -.
DR   KEGG; eco:b4278; -.
DR   PATRIC; fig|1411691.4.peg.2425; -.
DR   EchoBASE; EB4735; -.
DR   eggNOG; COG3385; Bacteria.
DR   HOGENOM; CLU_028400_1_1_6; -.
DR   InParanoid; P03835; -.
DR   OMA; PAEQVVW; -.
DR   PhylomeDB; P03835; -.
DR   BioCyc; EcoCyc:G7900-MON; -.
DR   PRO; PR:P03835; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004803; F:transposase activity; IEA:InterPro.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IEA:InterPro.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR002559; Transposase_11.
DR   InterPro; IPR024473; Transposases_IS4_N.
DR   Pfam; PF01609; DDE_Tnp_1; 1.
DR   Pfam; PF13006; Nterm_IS4; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   3: Inferred from homology;
KW   DNA recombination; DNA-binding; Reference proteome; Transposable element;
KW   Transposition.
FT   CHAIN           1..442
FT                   /note="Transposase InsG for insertion sequence element IS4"
FT                   /id="PRO_0000173291"
SQ   SEQUENCE   442 AA;  50386 MW;  0B9CAAA0FFAC724E CRC64;
     MHIGQALDLV SRYDSLRNPL TSLGDYLDPE LISRCLAESG TVTLRKRRLP LEMMVWCIVG
     MALERKEPLH QIVNRLDIML PGNRPFVAPS AVIQARQRLG SEAVRRVFTK TAQLWHNATP
     HPHWCGLTLL AIDGVFWRTP DTPENDAAFP RQTHAGNPAL YPQVKMVCQM ELTSHLLTAA
     AFGTMKNSEN ELAEQLIEQT GDNTLTLMDK GYYSLGLLNA WSLAGEHRHW MIPLRKGAQY
     EEIRKLGKGD HLVKLKTSPQ ARKKWPGLGN EVTARLLTVT RKGKVCHLLT SMTDAMRFPG
     GEMGDLYSHR WEIELGYREI KQTMQRSRLT LRSKKPELVE QELWGVLLAY NLVRYQMIKM
     AEHLKGYWPN QLSFSESCGM VMRMLMTLQG ASPGRIPELM RDLASMGQLV KLPTRRERAF
     PRVVKERPWK YPTAPKKSQS VA