ID   INSF1_ECOLI             Reviewed;         288 AA.
AC   P0CF79; O08009; O08012; O08304; P05822; P77673; Q2MBI8;
DT   18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Transposase InsF for insertion sequence IS3A;
GN   Name=insF1; OrderedLocusNames=b0299, JW0293;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2987844; DOI=10.1093/nar/13.6.2127;
RA   Timmerman K.P., Tu C.P.D.;
RT   "Complete sequence of IS3.";
RL   Nucleic Acids Res. 13:2127-2139(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Involved in the transposition of the insertion sequence IS3.
CC   -!- SIMILARITY: Belongs to the transposase IS3/IS150/IS904 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X02311; CAB37974.1; -; Genomic_DNA.
DR   EMBL; U73857; AAB18027.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73402.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76084.1; -; Genomic_DNA.
DR   PIR; C64756; TQECI3.
DR   RefSeq; NP_061381.1; NC_002483.1.
DR   RefSeq; NP_414833.1; NC_000913.3.
DR   RefSeq; NP_414906.1; NC_000913.3.
DR   RefSeq; NP_415073.1; NC_000913.3.
DR   RefSeq; NP_415545.1; NC_000913.3.
DR   RefSeq; NP_416593.1; NC_000913.3.
DR   RefSeq; WP_000878218.1; NZ_CP011343.2.
DR   AlphaFoldDB; P0CF79; -.
DR   STRING; 511145.b0299; -.
DR   PaxDb; P0CF79; -.
DR   PRIDE; P0CF79; -.
DR   EnsemblBacteria; AAC73402; AAC73402; b0299.
DR   EnsemblBacteria; BAE76084; BAE76084; BAE76084.
DR   GeneID; 944948; -.
DR   GeneID; 945036; -.
DR   GeneID; 945587; -.
DR   GeneID; 946629; -.
DR   GeneID; 947988; -.
DR   KEGG; ecj:JW0293; -.
DR   KEGG; eco:b0299; -.
DR   KEGG; eco:b0372; -.
DR   KEGG; eco:b0541; -.
DR   KEGG; eco:b1026; -.
DR   KEGG; eco:b2089; -.
DR   EchoBASE; EB4710; -.
DR   eggNOG; COG2801; Bacteria.
DR   HOGENOM; CLU_027402_4_2_6; -.
DR   InParanoid; P0CF79; -.
DR   OMA; CARYNTT; -.
DR   PhylomeDB; P0CF79; -.
DR   BioCyc; EcoCyc:G7126-MON; -.
DR   PRO; PR:P0CF79; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0032196; P:transposition; IDA:EcoCyc.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR025948; HTH-like_dom.
DR   InterPro; IPR038965; InsF-like.
DR   InterPro; IPR001584; Integrase_cat-core.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR46889:SF1; PTHR46889:SF1; 1.
DR   Pfam; PF13276; HTH_21; 1.
DR   Pfam; PF00665; rve; 1.
DR   Pfam; PF13333; rve_2; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50994; INTEGRASE; 1.
PE   3: Inferred from homology;
KW   DNA recombination; DNA-binding; Reference proteome; Transposable element;
KW   Transposition.
FT   CHAIN           1..288
FT                   /note="Transposase InsF for insertion sequence IS3A"
FT                   /id="PRO_0000075415"
FT   DOMAIN          124..287
FT                   /note="Integrase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
SQ   SEQUENCE   288 AA;  33540 MW;  9237EA662E021EB4 CRC64;
     MKYVFIEKHQ AEFSIKAMCR VLRVARSGWY TWCQRRTRIS TRQQFRQHCD SVVLAAFTRS
     KQRYGAPRLT DELRAQGYPF NVKTVAASLR RQGLRAKASR KFSPVSYRAH GLPVSENLLE
     QDFYASGPNQ KWAGDITYLR TDEGWLYLAV VIDLWSRAVI GWSMSPRMTA QLACDALQMA
     LWRRKRPRNV IVHTDRGGQY CSADYQAQLK RHNLRGSMSA KGCCYDNACV ESFFHSLKVE
     CIHGEHFISR EIMRATVFNY IECDYNRWRR HSWCGGLSPE QFENKNLA