INKA1_HUMAN
ID INKA1_HUMAN Reviewed; 287 AA.
AC Q96EL1;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-MAY-2019, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=PAK4-inhibitor INKA1 {ECO:0000305};
DE AltName: Full=Induced in neural crest by AP2-alpha protein homolog {ECO:0000303|PubMed:17314132};
DE Short=HInca {ECO:0000303|PubMed:17314132};
DE AltName: Full=Inka box actin regulator 1 {ECO:0000312|HGNC:HGNC:32480};
GN Name=INKA1 {ECO:0000312|HGNC:HGNC:32480};
GN Synonyms=C3orf54 {ECO:0000312|HGNC:HGNC:32480},
GN FAM212A {ECO:0000312|HGNC:HGNC:32480};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION.
RX PubMed=17314132; DOI=10.1242/dev.02813;
RA Luo T., Xu Y., Hoffman T.L., Zhang T., Schilling T., Sargent T.D.;
RT "Inca: a novel p21-activated kinase-associated protein required for cranial
RT neural crest development.";
RL Development 134:1279-1289(2007).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.06 ANGSTROMS) OF 169-192 IN COMPLEX WITH PAK4,
RP FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AND INTERACTION WITH PAK4.
RX PubMed=26607847; DOI=10.1038/ncomms9681;
RA Baskaran Y., Ang K.C., Anekal P.V., Chan W.L., Grimes J.M., Manser E.,
RA Robinson R.C.;
RT "An in cellulo-derived structure of PAK4 in complex with its inhibitor
RT Inka1.";
RL Nat. Commun. 6:8681-8681(2015).
CC -!- FUNCTION: Inhibitor of the serine/threonine-protein kinase PAK4
CC (PubMed:26607847). Acts by binding PAK4 in a substrate-like manner,
CC inhibiting the protein kinase activity (PubMed:26607847).
CC {ECO:0000269|PubMed:26607847}.
CC -!- SUBUNIT: Interacts with PAK4 (PubMed:26607847).
CC {ECO:0000269|PubMed:26607847}.
CC -!- INTERACTION:
CC Q96EL1; J3KQ12: BSCL2; NbExp=3; IntAct=EBI-10285157, EBI-11532900;
CC Q96EL1; Q9NX04: C1orf109; NbExp=3; IntAct=EBI-10285157, EBI-8643161;
CC Q96EL1; P26196: DDX6; NbExp=3; IntAct=EBI-10285157, EBI-351257;
CC Q96EL1; G5E9A7: DMWD; NbExp=3; IntAct=EBI-10285157, EBI-10976677;
CC Q96EL1; O95872: GPANK1; NbExp=3; IntAct=EBI-10285157, EBI-751540;
CC Q96EL1; Q9HAQ2: KIF9; NbExp=3; IntAct=EBI-10285157, EBI-8472129;
CC Q96EL1; P02545: LMNA; NbExp=3; IntAct=EBI-10285157, EBI-351935;
CC Q96EL1; O96013: PAK4; NbExp=3; IntAct=EBI-10285157, EBI-713738;
CC Q96EL1; Q9P286: PAK5; NbExp=5; IntAct=EBI-10285157, EBI-741896;
CC Q96EL1; O00628-2: PEX7; NbExp=3; IntAct=EBI-10285157, EBI-25882083;
CC Q96EL1; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-10285157, EBI-5235340;
CC Q96EL1; Q8TAU3: ZNF417; NbExp=3; IntAct=EBI-10285157, EBI-740727;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:26607847}. Cytoplasm
CC {ECO:0000269|PubMed:26607847}. Note=Mainly nuclear (PubMed:26607847).
CC Relocalizes to the cytoplasm following interaction with PAK4
CC (PubMed:26607847). {ECO:0000269|PubMed:26607847}.
CC -!- DOMAIN: Contains 2 Inka boxes (also named iBox or inca box)
CC (PubMed:26607847). The Inka boxes bind and inhibit PAK4 by binding a
CC substrate-like manner (PubMed:26607847). {ECO:0000269|PubMed:26607847}.
CC -!- SIMILARITY: Belongs to the INKA family. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-3 is the initiator.
CC {ECO:0000305}.
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DR EMBL; AC139451; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC012170; AAH12170.2; -; mRNA.
DR RefSeq; NP_976248.1; NM_203370.1.
DR PDB; 4XBR; X-ray; 2.94 A; A=168-205.
DR PDB; 4XBU; X-ray; 2.06 A; B=169-192.
DR PDBsum; 4XBR; -.
DR PDBsum; 4XBU; -.
DR AlphaFoldDB; Q96EL1; -.
DR SMR; Q96EL1; -.
DR BioGRID; 132984; 18.
DR IntAct; Q96EL1; 14.
DR STRING; 9606.ENSP00000329735; -.
DR iPTMnet; Q96EL1; -.
DR PhosphoSitePlus; Q96EL1; -.
DR BioMuta; FAM212A; -.
DR EPD; Q96EL1; -.
DR jPOST; Q96EL1; -.
DR MassIVE; Q96EL1; -.
DR PaxDb; Q96EL1; -.
DR PeptideAtlas; Q96EL1; -.
DR PRIDE; Q96EL1; -.
DR DNASU; 389119; -.
DR GeneID; 389119; -.
DR KEGG; hsa:389119; -.
DR UCSC; uc003cxq.1; human.
DR CTD; 389119; -.
DR DisGeNET; 389119; -.
DR GeneCards; INKA1; -.
DR HGNC; HGNC:32480; INKA1.
DR neXtProt; NX_Q96EL1; -.
DR PharmGKB; PA143485330; -.
DR eggNOG; ENOG502R5BS; Eukaryota.
DR HOGENOM; CLU_077157_0_0_1; -.
DR InParanoid; Q96EL1; -.
DR OrthoDB; 1263048at2759; -.
DR PhylomeDB; Q96EL1; -.
DR TreeFam; TF332839; -.
DR PathwayCommons; Q96EL1; -.
DR SignaLink; Q96EL1; -.
DR BioGRID-ORCS; 389119; 12 hits in 1079 CRISPR screens.
DR GenomeRNAi; 389119; -.
DR Pharos; Q96EL1; Tdark.
DR PRO; PR:Q96EL1; -.
DR Proteomes; UP000005640; Unplaced.
DR RNAct; Q96EL1; protein.
DR Genevisible; Q96EL1; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0019901; F:protein kinase binding; IPI:UniProtKB.
DR GO; GO:0030291; F:protein serine/threonine kinase inhibitor activity; IDA:UniProtKB.
DR InterPro; IPR029267; FAM212.
DR InterPro; IPR039201; Inka.
DR PANTHER; PTHR28615; PTHR28615; 1.
DR Pfam; PF15342; FAM212; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..287
FT /note="PAK4-inhibitor INKA1"
FT /id="PRO_0000239717"
FT REGION 22..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 138..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 168..205
FT /note="Inka box 1"
FT /evidence="ECO:0000269|PubMed:26607847"
FT REGION 261..287
FT /note="Inka box 2"
FT /evidence="ECO:0000269|PubMed:26607847"
FT COMPBIAS 30..47
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 190..192
FT /evidence="ECO:0007829|PDB:4XBR"
FT HELIX 193..196
FT /evidence="ECO:0007829|PDB:4XBR"
SQ SEQUENCE 287 AA; 31574 MW; D1A85638B948DF5C CRC64;
MDMHSARLDS FLSQLRWELL CGRDTGSPSM PGPLQPTSQT GPDVQPSHQL RASGALEEDS
VCCVEEEEEE EEEAVVTEDR DAALGGPREH ALDWDSGFSE VSGSTWREEE LPVSQRPAPS
AQPLRRQCLS VSGLPMPSRA PVASVPPVHH PRPKSTPDAC LEHWQGLEAE DWTAALLNRG
RSRQPLVLGD NCFADLVHNW MELPETGSEG GDGGGHRARA RPPQFLLGLS EQLRRRLARA
RRTAMAGKRL SCPPRPEPEL PADVSRFAAL MSCRSRQPII CNDVSYL
