INH3_ARATH
ID INH3_ARATH Reviewed; 107 AA.
AC Q8S8F7;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Protein phosphatase 1 regulatory subunit INH3 {ECO:0000305};
DE AltName: Full=Protein INHIBITOR-3 {ECO:0000303|PubMed:19329567};
DE Short=AtINH3 {ECO:0000303|PubMed:19329567};
GN Name=INH3 {ECO:0000303|PubMed:19329567};
GN OrderedLocusNames=At2g31305 {ECO:0000312|Araport:AT2G31305};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, INTERACTION WITH PROTEIN PHOSPHATASE 1, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF TRP-43.
RX PubMed=19329567; DOI=10.1104/pp.109.135335;
RA Takemiya A., Ariyoshi C., Shimazaki K.;
RT "Identification and functional characterization of inhibitor-3, a
RT regulatory subunit of protein phosphatase 1 in plants.";
RL Plant Physiol. 150:144-156(2009).
CC -!- FUNCTION: Inhibitor of protein-phosphatase 1 (PP1). Binds to and
CC inhibits PP1 activity. Required for early embryogenesis progression.
CC {ECO:0000269|PubMed:19329567}.
CC -!- SUBUNIT: Interacts with protein phosphatase 1.
CC {ECO:0000269|PubMed:19329567}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, cotyledons, leaves, flowers and
CC embryos. {ECO:0000269|PubMed:19329567}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality due to embryo development
CC arrest at globular stage. {ECO:0000269|PubMed:19329567}.
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DR EMBL; AC006593; AAM15305.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08525.1; -; Genomic_DNA.
DR EMBL; BT025702; ABF82605.1; -; mRNA.
DR EMBL; AY086700; AAM63754.1; -; mRNA.
DR RefSeq; NP_565720.1; NM_128687.2.
DR AlphaFoldDB; Q8S8F7; -.
DR STRING; 3702.AT2G31305.1; -.
DR PaxDb; Q8S8F7; -.
DR PRIDE; Q8S8F7; -.
DR ProteomicsDB; 228851; -.
DR EnsemblPlants; AT2G31305.1; AT2G31305.1; AT2G31305.
DR GeneID; 817688; -.
DR Gramene; AT2G31305.1; AT2G31305.1; AT2G31305.
DR KEGG; ath:AT2G31305; -.
DR Araport; AT2G31305; -.
DR TAIR; locus:505006282; AT2G31305.
DR eggNOG; KOG4102; Eukaryota.
DR HOGENOM; CLU_098333_4_2_1; -.
DR InParanoid; Q8S8F7; -.
DR OMA; PRMFGES; -.
DR PhylomeDB; Q8S8F7; -.
DR PRO; PR:Q8S8F7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8S8F7; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000164; C:protein phosphatase type 1 complex; IDA:TAIR.
DR GO; GO:0008157; F:protein phosphatase 1 binding; IBA:GO_Central.
DR GO; GO:0004865; F:protein serine/threonine phosphatase inhibitor activity; IBA:GO_Central.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0032515; P:negative regulation of phosphoprotein phosphatase activity; IDA:TAIR.
DR InterPro; IPR011107; PPI_Ypi1.
DR PANTHER; PTHR20835; PTHR20835; 1.
DR Pfam; PF07491; PPI_Ypi1; 1.
PE 1: Evidence at protein level;
KW Protein phosphatase inhibitor; Reference proteome.
FT CHAIN 1..107
FT /note="Protein phosphatase 1 regulatory subunit INH3"
FT /id="PRO_0000442228"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 69..107
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..97
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 43
FT /note="W->A: Loss of binding to protein-phosphatase 1
FT (PP1)."
FT /evidence="ECO:0000269|PubMed:19329567"
SQ SEQUENCE 107 AA; 12104 MW; D69DE3FEB4B95945 CRC64;
MSTATRPSSS ATTSVILENP VSQSQPTERL VLRLNRKKKK VSWKDGTVDN EFMQKKSSKK
CCIFHKQKPF DEDDSEEEDD NNHHCDHNHE HSESGEASSS NDSKAVD
