ID   IMMN_ECOLX              Reviewed;         131 AA.
AC   P09182;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Colicin-N immunity protein;
DE   AltName: Full=Microcin-N immunity protein;
GN   Name=cni;
OS   Escherichia coli.
OG   Plasmid ColN pCHAP4.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3280946; DOI=10.1007/bf00330613;
RA   Pugsley A.P.;
RT   "The immunity and lysis genes of ColN plasmid pCHAP4.";
RL   Mol. Gen. Genet. 211:335-341(1988).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; X06933; CAA30020.1; -; Genomic_DNA.
DR   PIR; S01761; IMECN4.
DR   AlphaFoldDB; P09182; -.
DR   TCDB; 8.B.24.1.2; the colicin immunity protein (colip) functional family.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015643; F:toxic substance binding; IEA:InterPro.
DR   GO; GO:0030153; P:bacteriocin immunity; IEA:UniProtKB-KW.
DR   InterPro; IPR005557; Colicin_im.
DR   Pfam; PF03857; Colicin_im; 1.
DR   PIRSF; PIRSF003003; Colicin_im; 1.
PE   4: Predicted;
KW   Bacteriocin immunity; Cell membrane; Membrane; Plasmid; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..131
FT                   /note="Colicin-N immunity protein"
FT                   /id="PRO_0000218698"
FT   TRANSMEM        66..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        104..124
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   131 AA;  15245 MW;  D17EF9FC264DB96C CRC64;
     MHNTLLEKII AYLSLPGFHS LNNPPLSEAF NLYVHTAPLA ATSLFIFTHK ELELKPKSSP
     LRALKILTPF TILYISMIYC FLLTDTELTL SSKTFVLIVK KRSVFVFFLY NTIYWDIYIH
     IFVLLVPYRN I