ID   IML2_YEAS7              Reviewed;         731 AA.
AC   A6ZPP2;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   03-AUG-2022, entry version 48.
DE   RecName: Full=Inclusion body clearance protein IML2;
DE   AltName: Full=Increased minichromosome loss protein 2;
GN   Name=IML2; ORFNames=SCY_2852;
OS   Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=307796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YJM789;
RX   PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA   Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA   Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA   Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA   Steinmetz L.M.;
RT   "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT   strain YJM789.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC   -!- FUNCTION: Inclusion body (IB) resident protein that interacts strongly
CC       with lipid droplet (LD) proteins. Involved in LD-mediated IB clearing
CC       after protein folding stress, probably by enabling access to the IBs of
CC       an LD-stored soluble sterol derivative that acts as chaperone in
CC       inclusion clearing. {ECO:0000250|UniProtKB:P47031}.
CC   -!- SUBUNIT: Interacts with lipid droplet proteins PET10 and PDR16.
CC       {ECO:0000250|UniProtKB:P47031}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P47031}. Nucleus
CC       {ECO:0000250|UniProtKB:P47031}. Note=Localized exclusively in
CC       cytoplasmic inclusion bodies under protein folding stress conditions.
CC       {ECO:0000250|UniProtKB:P47031}.
CC   -!- SIMILARITY: Belongs to the IML2 family. {ECO:0000305}.
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DR   EMBL; AAFW02000038; EDN63495.1; -; Genomic_DNA.
DR   AlphaFoldDB; A6ZPP2; -.
DR   PRIDE; A6ZPP2; -.
DR   EnsemblFungi; EDN63495; EDN63495; SCY_2852.
DR   HOGENOM; CLU_014926_0_0_1; -.
DR   Proteomes; UP000007060; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR019412; Iml2/TPR_39.
DR   PANTHER; PTHR31859; PTHR31859; 1.
DR   Pfam; PF10300; DUF3808; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Nucleus; Phosphoprotein.
FT   CHAIN           1..731
FT                   /note="Inclusion body clearance protein IML2"
FT                   /id="PRO_0000333356"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        8..26
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         265
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P47031"
FT   MOD_RES         268
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P47031"
FT   MOD_RES         378
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P36114"
FT   MOD_RES         380
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P47031"
FT   MOD_RES         383
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P47031"
FT   MOD_RES         392
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P47031"
SQ   SEQUENCE   731 AA;  82548 MW;  63BC2D27B85FB1AF CRC64;
     MFRVFGSFGS KGNQSSGEEQ STKTKQVLKQ ANDFEIALKA MDFVLDDRTD EGLNLLKKAE
     METGSDQTIL TLARGVIEFL QATLSFETEE MKRAAITLGK AEQMSWKSKQ NAEKTNFRSS
     SIYPPGTVYA VTYTESCLLH ALLMLFSESM MEAAKALLKL RRAYTMLQDI MVTVKKAERS
     KNSSSPSPSE KSQESCGSFV SAETTFISVD IPYKLSSEDK SNPLLLEFAE KIYTMRMGRL
     SGAHIGNTPS FHRLRDDLGL QTTPSQASDR HSVSDDFDLE QATIDEFIHS GANLCYGILQ
     VVLSLLPPAI GAVLSIVGFK GSREEGLRLV WKAIKERNVH GCIGLLGLMF YYDGPFQFTD
     ADFDIPPNDN GSRALNKSRT NDSSLLPGYM DSATLLHPGK ILEDALLKAR ALFPNSALWL
     LNEAKMLAGK GRLRDSLALM DSIDVNSIRM RQVKSLMVFE RAILLVNLHE YNRAADDLIS
     LLDISDWSHA LYTYFAGCCY LENWRMTQLG LLNDGKEQFY KERARELIFD APSLLGKKTF
     KSKNLPLDRF MLRKVQQFNN MQKKLNLQEP LDSIATSPVH ELAYFYNGYN RMTENDLILT
     KKMLTEYHNP AIDSEDPDQE LIRNLLLSLT LRRLGDAERG LALLDDIVLP KIFYIQNGKV
     KYFKKTEDPW AYPAALYERA LFCWKLGGME SLNECREWLL RAQNYAADYE LSTRIGMKIK
     AALDRVENAL A