IMFS_LUFAE
ID IMFS_LUFAE Reviewed; 759 AA.
AC Q948R6;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Isomultiflorenol synthase;
DE Short=LcIMS1;
DE EC=5.4.99.36;
GN Name=IMS1;
OS Luffa aegyptiaca (Sponge gourd) (Luffa cylindrica).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Cucurbitales; Cucurbitaceae; Sicyoeae; Luffa.
OX NCBI_TaxID=3670;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND INDUCTION.
RX PubMed=11733028; DOI=10.1046/j.0014-2956.2001.02588.x;
RA Hayashi H., Huang P., Inoue K., Hiraoka N., Ikeshiro Y., Yazaki K.,
RA Tanaka S., Kushiro T., Shibuya M., Ebizuka Y.;
RT "Molecular cloning and characterization of isomultiflorenol synthase, a new
RT triterpene synthase from Luffa cylindrica, involved in biosynthesis of
RT bryonolic acid.";
RL Eur. J. Biochem. 268:6311-6317(2001).
CC -!- FUNCTION: Oxidosqualene cyclase involved in the biosynthesis of
CC bryonolic acid. Converts oxidosqualene to isomultiflorenol.
CC {ECO:0000269|PubMed:11733028}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-2,3-epoxysqualene = isomultiflorenol;
CC Xref=Rhea:RHEA:30451, ChEBI:CHEBI:15441, ChEBI:CHEBI:62262;
CC EC=5.4.99.36; Evidence={ECO:0000269|PubMed:11733028};
CC -!- MISCELLANEOUS: In cultured cells, IMS1 mRNA expression is high between
CC days 16 and 20 and precedes the accumulation of bryonolic acid.
CC -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC {ECO:0000305}.
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DR EMBL; AB058643; BAB68529.1; -; mRNA.
DR AlphaFoldDB; Q948R6; -.
DR SMR; Q948R6; -.
DR KEGG; ag:BAB68529; -.
DR BRENDA; 5.4.99.36; 3083.
DR GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR GO; GO:0031559; F:oxidosqualene cyclase activity; IEA:UniProt.
DR GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd02892; SQCY_1; 1.
DR InterPro; IPR032696; SQ_cyclase_C.
DR InterPro; IPR032697; SQ_cyclase_N.
DR InterPro; IPR018333; Squalene_cyclase.
DR InterPro; IPR002365; Terpene_synthase_CS.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR PANTHER; PTHR11764; PTHR11764; 1.
DR Pfam; PF13243; SQHop_cyclase_C; 1.
DR Pfam; PF13249; SQHop_cyclase_N; 1.
DR SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE 1: Evidence at protein level;
KW Isomerase; Repeat.
FT CHAIN 1..759
FT /note="Isomultiflorenol synthase"
FT /id="PRO_0000412991"
FT REPEAT 148..189
FT /note="PFTB 1"
FT REPEAT 588..628
FT /note="PFTB 2"
FT REPEAT 637..678
FT /note="PFTB 3"
FT REPEAT 699..743
FT /note="PFTB 4"
FT ACT_SITE 482
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:P48449"
SQ SEQUENCE 759 AA; 87840 MW; 8215E88FB3A43359 CRC64;
MWRLKVADGG NDPYIYSMNN FIGRQIWEFD PNAGTPEERA EIERLRHHFT KNRHKGFPSA
DLLWRVQLLR EKNFKQSIPA VKVGDGEEIS YEMALDAMRR GAHFLAAIQA SDGHWPSETS
GPLFYVCPLL ICMYIMGFMD KVFSPEHKKE MMRYIYNHQN EDGGWGLHVG GHSNMFCTTF
NYISLRLLGE EPDVEAVCKA RNWIHDHDGV TSILSWGKTW LSILNVFDWS ASNPMPPEYW
MLPTWVPIHP SNMMCYTRIT YMPMSYLYGK RFQAPLTPLV LQLRDELHTQ PYDQINWRKV
RHMCATEDLY FPHPFVQDLL WDTLYLLSEP LMTRWPFNKL IRQKALNETM RHIHYEDENS
RYITIGCVEK PLCMLACWVE DPNSEYVKKH LARIPDYLWM AEDGMKMQSF GSQSWDAALA
MQALLSCNIT REIGSVLNSG HDFIKNSQVR NNPPGDYKSM FRYMSKGSWT FSDCDHGWQV
SDCTAENLKC CLLLSLLPPD IVGEKMEPER FYDAVNVILN MQSKNGGLPA WEPASSYYWM
EWLNPVEFLE DLIIEHQHVE CTSSALQAIL LFRKQYPGHR RKEINNFINK AVQFLQDIQL
PDGSWYGNWG ICYTYGTWFA LKALSMAGKT YENCEAVRKG ANFLRKIQNP EGGFGESYLS
CPYKRYIPLD GKRSNLVQTA WGMMGLICAG QADVDPTPIH RAAKLLINSQ TEDGDFPQEE
ITGEFFKNCT LHFAAFREVF PVMALGEYCN KVPLPSKKK
