AP5S1_MOUSE
ID AP5S1_MOUSE Reviewed; 170 AA.
AC Q9D742; A2ANC8;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=AP-5 complex subunit sigma-1;
DE AltName: Full=Adaptor-related protein complex 5 sigma subunit;
DE Short=Sigma5;
GN Name=Ap5s1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: As part of AP-5, a probable fifth adaptor protein complex it
CC may be involved in endosomal transport. {ECO:0000250}.
CC -!- SUBUNIT: Probably part of the adaptor protein complex 5 (AP-5) a
CC tetramer composed of AP5B1, AP5M1, AP5S1 and AP5Z1. Interacts with
CC ZFYVE26 and SPG11 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol. Late endosome membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Lysosome membrane {ECO:0000250}; Peripheral
CC membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
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DR EMBL; AK009634; BAB26405.1; -; mRNA.
DR EMBL; AL808128; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS71147.1; -.
DR RefSeq; NP_001277961.1; NM_001291032.1.
DR RefSeq; NP_081405.2; NM_027129.3.
DR AlphaFoldDB; Q9D742; -.
DR ComplexPortal; CPX-5182; AP-5 Adaptor complex.
DR STRING; 10090.ENSMUSP00000086933; -.
DR iPTMnet; Q9D742; -.
DR PhosphoSitePlus; Q9D742; -.
DR MaxQB; Q9D742; -.
DR PaxDb; Q9D742; -.
DR PRIDE; Q9D742; -.
DR ProteomicsDB; 296362; -.
DR Antibodypedia; 64737; 11 antibodies from 7 providers.
DR DNASU; 69596; -.
DR Ensembl; ENSMUST00000110210; ENSMUSP00000105839; ENSMUSG00000068264.
DR GeneID; 69596; -.
DR KEGG; mmu:69596; -.
DR UCSC; uc056zpo.1; mouse.
DR CTD; 55317; -.
DR MGI; MGI:1916846; Ap5s1.
DR VEuPathDB; HostDB:ENSMUSG00000068264; -.
DR eggNOG; ENOG502RZ3F; Eukaryota.
DR GeneTree; ENSGT00390000013178; -.
DR InParanoid; Q9D742; -.
DR OrthoDB; 1469638at2759; -.
DR BioGRID-ORCS; 69596; 3 hits in 108 CRISPR screens.
DR ChiTaRS; Ap5s1; mouse.
DR PRO; PR:Q9D742; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9D742; protein.
DR Bgee; ENSMUSG00000068264; Expressed in hindlimb stylopod muscle and 215 other tissues.
DR ExpressionAtlas; Q9D742; baseline and differential.
DR Genevisible; Q9D742; MM.
DR GO; GO:0044599; C:AP-5 adaptor complex; IC:ComplexPortal.
DR GO; GO:0030119; C:AP-type membrane coat adaptor complex; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005770; C:late endosome; ISS:UniProtKB.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; ISS:UniProtKB.
DR GO; GO:0016197; P:endosomal transport; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0016192; P:vesicle-mediated transport; IC:ComplexPortal.
DR InterPro; IPR029392; AP-5_subunit_s1.
DR PANTHER; PTHR16120; PTHR16120; 1.
DR Pfam; PF15001; AP-5_subunit_s1; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA damage; DNA repair; Endosome; Lysosome; Membrane;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..170
FT /note="AP-5 complex subunit sigma-1"
FT /id="PRO_0000079424"
SQ SEQUENCE 170 AA; 18806 MW; C897579BFFF8F75B CRC64;
MVHAFLIHTL RAPNLEDTGL CRVLYSCVFG AEKSPDDPRS HGAERDRLFR KEQILAVARQ
VESLCRLQQQ AAGCSSTDLQ PQFSAEPVSL HEAPHGAFHL AAGDPFQEPR TVLWLGILSL
GFALVLDTHE NLLLAERTLR LLARLLLDHL RLLTPAVSTG AAYCRAHPSR
