AP5M1_MOUSE
ID AP5M1_MOUSE Reviewed; 490 AA.
AC Q8BJ63; Q3TDF2; Q3TPT5; Q8BII2; Q8BJ31; Q8BJ58; Q8BY11; Q8R5B1;
DT 03-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=AP-5 complex subunit mu-1;
DE AltName: Full=Adaptor-related protein complex 5 subunit mu-1;
DE Short=Mu5;
DE AltName: Full=Mu-2-related death-inducing protein;
DE Short=MuD;
GN Name=Ap5m1; Synonyms=Mudeng;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=C57BL/6J, and NOD;
RC TISSUE=Bone, Cerebellum, Dendritic cell, Embryonic spinal ganglion,
RC Embryonic testis, Hippocampus, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Czech II; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=18395520; DOI=10.1016/j.bbrc.2008.03.139;
RA Lee M.-R., Shin J.N., Moon A.R., Park S.-Y., Hong G., Lee M.-J., Yun C.-W.,
RA Seol D.-W., Piya S., Bae J., Oh J.-W., Kim T.-H.;
RT "A novel protein, MUDENG, induces cell death in cytotoxic T cells.";
RL Biochem. Biophys. Res. Commun. 370:504-508(2008).
CC -!- FUNCTION: As part of AP-5, a probable fifth adaptor protein complex it
CC may be involved in endosomal transport. {ECO:0000250}.
CC -!- SUBUNIT: Probably part of the adaptor protein complex 5 (AP-5) a
CC tetramer composed of AP5B1, AP5M1, AP5S1 and AP5Z1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol. Late endosome membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Lysosome membrane {ECO:0000250}; Peripheral
CC membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC Note=May cycle on and off membranes. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8BJ63-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BJ63-2; Sequence=VSP_008411, VSP_008412;
CC Name=3;
CC IsoId=Q8BJ63-3; Sequence=VSP_008409, VSP_008410;
CC -!- TISSUE SPECIFICITY: Widely expressed, including in small intestine and
CC testis. In small intestine, highly expressed in cytoplasm of villi
CC epithelial cells and internal glands. In testis, selectively expressed
CC in maturing sperm cells (at protein level).
CC {ECO:0000269|PubMed:18395520}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform 3]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the adaptor complexes medium subunit family.
CC {ECO:0000305}.
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DR EMBL; AK030928; BAC27187.1; -; mRNA.
DR EMBL; AK031527; BAC27435.1; -; mRNA.
DR EMBL; AK036465; BAC29441.1; -; mRNA.
DR EMBL; AK042544; BAC31288.2; -; mRNA.
DR EMBL; AK051372; BAC34616.1; -; mRNA.
DR EMBL; AK154166; BAE32417.1; -; mRNA.
DR EMBL; AK164148; BAE37650.1; -; mRNA.
DR EMBL; AK170232; BAE41650.1; -; mRNA.
DR EMBL; CH466605; EDL20784.1; -; Genomic_DNA.
DR EMBL; BC023094; AAH23094.1; -; mRNA.
DR CCDS; CCDS26995.1; -. [Q8BJ63-1]
DR RefSeq; NP_653118.3; NM_144535.4.
DR RefSeq; XP_006519685.1; XM_006519622.3.
DR RefSeq; XP_006519686.1; XM_006519623.3.
DR RefSeq; XP_011243514.1; XM_011245212.2.
DR AlphaFoldDB; Q8BJ63; -.
DR SMR; Q8BJ63; -.
DR BioGRID; 216709; 1.
DR ComplexPortal; CPX-5182; AP-5 Adaptor complex.
DR STRING; 10090.ENSMUSP00000046536; -.
DR iPTMnet; Q8BJ63; -.
DR PhosphoSitePlus; Q8BJ63; -.
DR MaxQB; Q8BJ63; -.
DR PaxDb; Q8BJ63; -.
DR PeptideAtlas; Q8BJ63; -.
DR PRIDE; Q8BJ63; -.
DR ProteomicsDB; 296214; -. [Q8BJ63-1]
DR ProteomicsDB; 296215; -. [Q8BJ63-2]
DR ProteomicsDB; 296216; -. [Q8BJ63-3]
DR Antibodypedia; 68208; 58 antibodies from 17 providers.
DR DNASU; 74385; -.
DR Ensembl; ENSMUST00000228238; ENSMUSP00000154186; ENSMUSG00000036291. [Q8BJ63-3]
DR GeneID; 74385; -.
DR KEGG; mmu:74385; -.
DR UCSC; uc007tjx.2; mouse. [Q8BJ63-3]
DR UCSC; uc007tjy.2; mouse. [Q8BJ63-2]
DR UCSC; uc007tjz.2; mouse. [Q8BJ63-1]
DR CTD; 55745; -.
DR MGI; MGI:1921635; Ap5m1.
DR VEuPathDB; HostDB:ENSMUSG00000036291; -.
DR eggNOG; KOG0937; Eukaryota.
DR GeneTree; ENSGT00390000006191; -.
DR InParanoid; Q8BJ63; -.
DR OrthoDB; 395662at2759; -.
DR PhylomeDB; Q8BJ63; -.
DR TreeFam; TF331963; -.
DR BioGRID-ORCS; 74385; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Ap5m1; mouse.
DR PRO; PR:Q8BJ63; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8BJ63; protein.
DR Bgee; ENSMUSG00000036291; Expressed in paneth cell and 246 other tissues.
DR ExpressionAtlas; Q8BJ63; baseline and differential.
DR GO; GO:0044599; C:AP-5 adaptor complex; IC:ComplexPortal.
DR GO; GO:0030119; C:AP-type membrane coat adaptor complex; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0005770; C:late endosome; ISS:UniProtKB.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR GO; GO:0016020; C:membrane; ISS:UniProtKB.
DR GO; GO:0016197; P:endosomal transport; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0016192; P:vesicle-mediated transport; IC:ComplexPortal.
DR InterPro; IPR036168; AP2_Mu_C_sf.
DR InterPro; IPR039591; AP5M1.
DR InterPro; IPR028565; MHD.
DR PANTHER; PTHR16082; PTHR16082; 1.
DR Pfam; PF00928; Adap_comp_sub; 1.
DR SUPFAM; SSF49447; SSF49447; 1.
DR PROSITE; PS51072; MHD; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Endosome; Lysosome; Membrane;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..490
FT /note="AP-5 complex subunit mu-1"
FT /id="PRO_0000193796"
FT DOMAIN 206..476
FT /note="MHD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00404"
FT VAR_SEQ 241..307
FT /note="CDLEGVMPAVTISLSLPTNGSPLQDIIVHPCVTSLDSAILTSSSIDTMDDSA
FT FSGPYKFPFTPPLES -> VRFSPGFALSYHFTLKNSQFCNLYFTFLNGVLVILSNSHE
FT ILCYLSVFLTNCLFNDYNVMNHPIALK (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_008409"
FT VAR_SEQ 308..490
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_008410"
FT VAR_SEQ 393..398
FT /note="QKFPKS -> EYGFVY (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_008411"
FT VAR_SEQ 399..490
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_008412"
FT CONFLICT 36
FT /note="A -> V (in Ref. 1; BAE41650/BAE32417 and 2;
FT EDL20784)"
FT /evidence="ECO:0000305"
FT CONFLICT 45
FT /note="V -> I (in Ref. 1; BAE41650/BAE32417 and 2;
FT EDL20784)"
FT /evidence="ECO:0000305"
FT CONFLICT 55
FT /note="L -> V (in Ref. 3; AAH23094)"
FT /evidence="ECO:0000305"
FT CONFLICT 157
FT /note="S -> K (in Ref. 3; AAH23094)"
FT /evidence="ECO:0000305"
FT CONFLICT 289
FT /note="D -> E (in Ref. 1; BAC27435/BAC34616/BAC27187/
FT BAE37650)"
FT /evidence="ECO:0000305"
FT CONFLICT 416
FT /note="K -> N (in Ref. 1; BAC34616)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 490 AA; 54337 MW; 4EF78F1D279C0BF3 CRC64;
MALRAVWLIR HEPGTPLGGT VRFSRRYPTV EKRAKAFNGM TYVPVPEDGP FLRALLFQLR
LLDDDKDFME RRDGCSRINK TSIYGLSVGG EELWPVIAFL RDSMIYASVP LVEQALSPRP
PLISISGVSQ GLELLLGIQD FLYSSQKNDT DLHTKLSQLP DLLLQACPLG TLLDANLQNS
LNSINSVSVT QPQKQPAWKV GAYKGKAQIS ISITETVKCM QYGKQDIADT WQVAGTVACK
CDLEGVMPAV TISLSLPTNG SPLQDIIVHP CVTSLDSAIL TSSSIDTMDD SAFSGPYKFP
FTPPLESFNL CHYTSQVPVP PILGSYHMKE EGVQLKVTVN FKLHESVRNN FEVCEAHIPF
YNRGPITHLE YKASFGQLEV FREKSLLVWI IGQKFPKSME ISLSGTLTFG VKGHNKQPFD
HICIGNTAYI KLNFRIADYT LTGCYADQHS VQVFASGKPK ISAYRKLISS DYYIWNSKAP
APVTYASLLP
