ID   HSD_MYCBO               Reviewed;         260 AA.
AC   P69166; A0A1R3Y004; Q10855; X2BJ87;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase {ECO:0000250|UniProtKB:P9WGT1};
DE            EC=1.1.1.53 {ECO:0000250|UniProtKB:P9WGT1};
GN   Name=fabG3; OrderedLocusNames=BQ2027_MB2025;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: Probably involved in steroid metabolism.
CC       {ECO:0000250|UniProtKB:P9WGT1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=androstan-3alpha,17beta-diol + NAD(+) = 17beta-
CC         hydroxyandrostanone + H(+) + NADH; Xref=Rhea:RHEA:22400,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:18011, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:85278; EC=1.1.1.53;
CC         Evidence={ECO:0000250|UniProtKB:P9WGT1};
CC   -!- PATHWAY: Lipid metabolism; steroid degradation.
CC       {ECO:0000250|UniProtKB:P9WGT1}.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:P9WGT1}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; LT708304; SIU00632.1; -; Genomic_DNA.
DR   RefSeq; NP_855675.1; NC_002945.3.
DR   RefSeq; WP_003410047.1; NC_002945.4.
DR   AlphaFoldDB; P69166; -.
DR   SMR; P69166; -.
DR   EnsemblBacteria; SIU00632; SIU00632; BQ2027_MB2025.
DR   PATRIC; fig|233413.5.peg.2224; -.
DR   OMA; GICEFKE; -.
DR   UniPathway; UPA00722; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0047044; F:androstan-3-alpha,17-beta-diol dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006706; P:steroid catabolic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Lipid metabolism; NAD; Oxidoreductase; Steroid metabolism.
FT   CHAIN           1..260
FT                   /note="3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase"
FT                   /id="PRO_0000054711"
FT   ACT_SITE        153
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         17..19
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         38
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         61..62
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         88
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         153
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         157
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         183..191
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
SQ   SEQUENCE   260 AA;  27030 MW;  0935A14ED36220B7 CRC64;
     MSGRLIGKVA LVSGGARGMG ASHVRAMVAE GAKVVFGDIL DEEGKAVAAE LADAARYVHL
     DVTQPAQWTA AVDTAVTAFG GLHVLVNNAG ILNIGTIEDY ALTEWQRILD VNLTGVFLGI
     RAVVKPMKEA GRGSIINISS IEGLAGTVAC HGYTATKFAV RGLTKSTALE LGPSGIRVNS
     IHPGLVKTPM TDWVPEDIFQ TALGRAAEPV EVSNLVVYLA SDESSYSTGA EFVVDGGTVA
     GLAHNDFGAV EVSSQPEWVT