HSA32_ARATH
ID HSA32_ARATH Reviewed; 286 AA.
AC Q8GWL1; O81899;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Protein HEAT-STRESS-ASSOCIATED 32 {ECO:0000303|PubMed:16500991};
DE Short=Heat-stress-associated 32-kDa protein {ECO:0000303|PubMed:16500991};
GN Name=HSA32 {ECO:0000303|PubMed:16500991};
GN OrderedLocusNames=At4g21320 {ECO:0000312|Araport:AT4G21320};
GN ORFNames=T6K22.50 {ECO:0000312|EMBL:CAA20197.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY HEAT SHOCK.
RC STRAIN=cv. Columbia;
RX PubMed=16500991; DOI=10.1104/pp.105.074898;
RA Charng Y.-Y., Liu H.-C., Liu N.-Y., Hsu F.-C., Ko S.-S.;
RT "Arabidopsis Hsa32, a novel heat shock protein, is essential for acquired
RT thermotolerance during long recovery after acclimation.";
RL Plant Physiol. 140:1297-1305(2006).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=17085506; DOI=10.1104/pp.106.091322;
RA Charng Y.-Y., Liu H.-C., Liu N.-Y., Chi W.-T., Wang C.-N., Chang S.-H.,
RA Wang T.-T.;
RT "A heat-inducible transcription factor, HsfA2, is required for extension of
RT acquired thermotolerance in Arabidopsis.";
RL Plant Physiol. 143:251-262(2007).
RN [6]
RP INDUCTION BY HEAT SHOCK.
RX PubMed=23073024; DOI=10.4161/psb.22092;
RA Shahnejat-Bushehri S., Mueller-Roeber B., Balazadeh S.;
RT "Arabidopsis NAC transcription factor JUNGBRUNNEN1 affects thermomemory-
RT associated genes and enhances heat stress tolerance in primed and unprimed
RT conditions.";
RL Plant Signal. Behav. 7:1518-1521(2012).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY CLPB1.
RC STRAIN=cv. Columbia;
RX PubMed=23439916; DOI=10.1104/pp.112.212589;
RA Wu T.-Y., Juan Y.-T., Hsu Y.-H., Wu S.-H., Liao H.-T., Fung R.W.M.,
RA Charng Y.-Y.;
RT "Interplay between heat shock proteins HSP101 and HSA32 prolongs heat
RT acclimation memory posttranscriptionally in Arabidopsis.";
RL Plant Physiol. 161:2075-2084(2013).
CC -!- FUNCTION: Transactivator required, together with HSP101, for long-term
CC acquired thermotolerance (LAT) maintenance, probably by regulating
CC heat-inducible genes expression, thus being a cellular component of
CC thermomemory. {ECO:0000269|PubMed:16500991,
CC ECO:0000269|PubMed:17085506}.
CC -!- INDUCTION: Accumulates after heat shock (HS) (PubMed:16500991,
CC PubMed:23073024). Enhanced translation during recovery after heat
CC treatment mediated by CLPB1 in a positive feedback loop
CC (PubMed:23439916). {ECO:0000269|PubMed:16500991,
CC ECO:0000269|PubMed:23073024, ECO:0000269|PubMed:23439916}.
CC -!- DISRUPTION PHENOTYPE: Normal growth and development under nonstress
CC conditions. Compromised acquired thermotolerance following a long
CC recovery period (> 24 h) after acclimation heat shock (HS) treatment,
CC but normal when challenged within a short recovery period
CC (PubMed:16500991, PubMed:17085506). Reduced expression of several
CC highly heat-inducible genes. Slight increased in sensitivity to HS
CC without acclimation (PubMed:17085506). Faster degradation of HSP101
CC (PubMed:23439916). {ECO:0000269|PubMed:16500991,
CC ECO:0000269|PubMed:17085506, ECO:0000269|PubMed:23439916}.
CC -!- SIMILARITY: Belongs to the phosphosulfolactate synthase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA20197.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g21320 has been split into 3 genes: At4g21320, At4g21323 and At4g21326.; Evidence={ECO:0000305};
CC Sequence=CAB79131.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g21320 has been split into 3 genes: At4g21320, At4g21323 and At4g21326.; Evidence={ECO:0000305};
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DR EMBL; AL031187; CAA20197.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161554; CAB79131.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84439.1; -; Genomic_DNA.
DR EMBL; AK118775; BAC43368.1; -; mRNA.
DR PIR; T05174; T05174.
DR RefSeq; NP_567623.2; NM_118250.5.
DR AlphaFoldDB; Q8GWL1; -.
DR SMR; Q8GWL1; -.
DR STRING; 3702.AT4G21320.1; -.
DR MEROPS; S08.A32; -.
DR PaxDb; Q8GWL1; -.
DR PRIDE; Q8GWL1; -.
DR ProteomicsDB; 232151; -.
DR DNASU; 827880; -.
DR EnsemblPlants; AT4G21320.1; AT4G21320.1; AT4G21320.
DR GeneID; 827880; -.
DR Gramene; AT4G21320.1; AT4G21320.1; AT4G21320.
DR KEGG; ath:AT4G21320; -.
DR Araport; AT4G21320; -.
DR TAIR; locus:2141196; AT4G21320.
DR eggNOG; ENOG502RBUH; Eukaryota.
DR HOGENOM; CLU_062679_0_0_1; -.
DR InParanoid; Q8GWL1; -.
DR OMA; MFEASNA; -.
DR OrthoDB; 987034at2759; -.
DR PhylomeDB; Q8GWL1; -.
DR PRO; PR:Q8GWL1; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8GWL1; baseline and differential.
DR GO; GO:0010286; P:heat acclimation; IMP:UniProtKB.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IMP:TAIR.
DR GO; GO:0009408; P:response to heat; IDA:UniProtKB.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR003830; ComA_synth.
DR InterPro; IPR036112; ComA_synth_sf.
DR Pfam; PF02679; ComA; 1.
DR SUPFAM; SSF102110; SSF102110; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Stress response.
FT CHAIN 1..286
FT /note="Protein HEAT-STRESS-ASSOCIATED 32"
FT /id="PRO_0000435166"
SQ SEQUENCE 286 AA; 32640 MW; B2E321577AF9DACB CRC64;
MAAYYRWKSF EENEDRPEKP RRYGVTEMRG PHYSVLSQNL LQEIFESMGQ FVDGLKFSGG
SNSLIPKSFI KQAIEMAHEH GVYVSTGDWA EHMLRSGPSA FKDYVEECKQ LGFDTIELNA
NLLEVPEETL LRYVRLIKNG GLRAKPMFAV KFNKSDIPGR NRAFGSYVVP EPRSSEFVED
IDLLIRKAER CLEAGADTIM IDADDVCKYA DSLRADIIAK VIGRLGIEKT MFEASDAKLV
EWFIKRYGPN VNLYVDHSQI MDLECLRGRH LGKDHQSVLS SSYFLF
