HNRL2_HUMAN
ID HNRL2_HUMAN Reviewed; 747 AA.
AC Q1KMD3; Q8N3B3;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Heterogeneous nuclear ribonucleoprotein U-like protein 2;
DE AltName: Full=Scaffold-attachment factor A2;
DE Short=SAF-A2;
GN Name=HNRNPUL2; Synonyms=HNRPUL2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1] {ECO:0000305, ECO:0000312|EMBL:ABF00125.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Herrmann F., Fackelmayer F.O.;
RT "Scaffold attachment factor A2 (SAF-A2), a mobile isoform of a
RT nucleoskeletal human protein.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 122-747.
RC TISSUE=Melanoma;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3] {ECO:0000305, ECO:0000312|EMBL:ABF00125.1}
RP PROTEIN SEQUENCE OF 161-174; 213-218; 226-237; 264-282; 298-315; 379-391;
RP 481-494; 497-506; 509-521; 558-570; 594-601; 721-733 AND 739-747,
RP PHOSPHORYLATION AT SER-228, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Ovarian carcinoma;
RA Bienvenut W.V., Lilla S., von Kriegsheim A., Lempens A., Kolch W.;
RL Submitted (DEC-2008) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [5]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-738 AND ARG-747, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Colon carcinoma;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- SUBUNIT: Binds to MLF1 and retains it in the nucleus.
CC {ECO:0000250|UniProtKB:Q00PI9}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q00PI9}.
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DR EMBL; DQ470474; ABF00125.1; -; mRNA.
DR EMBL; AL834470; CAD39129.2; -; mRNA.
DR CCDS; CCDS41659.1; -.
DR RefSeq; NP_001073027.1; NM_001079559.2.
DR AlphaFoldDB; Q1KMD3; -.
DR SMR; Q1KMD3; -.
DR BioGRID; 128685; 216.
DR IntAct; Q1KMD3; 62.
DR MINT; Q1KMD3; -.
DR STRING; 9606.ENSP00000301785; -.
DR GlyGen; Q1KMD3; 2 sites, 1 O-linked glycan (2 sites).
DR iPTMnet; Q1KMD3; -.
DR MetOSite; Q1KMD3; -.
DR PhosphoSitePlus; Q1KMD3; -.
DR SwissPalm; Q1KMD3; -.
DR BioMuta; HNRNPUL2; -.
DR DMDM; 121940728; -.
DR EPD; Q1KMD3; -.
DR jPOST; Q1KMD3; -.
DR MassIVE; Q1KMD3; -.
DR MaxQB; Q1KMD3; -.
DR PaxDb; Q1KMD3; -.
DR PeptideAtlas; Q1KMD3; -.
DR PRIDE; Q1KMD3; -.
DR ProteomicsDB; 61219; -.
DR Antibodypedia; 50593; 77 antibodies from 23 providers.
DR DNASU; 221092; -.
DR Ensembl; ENST00000301785.7; ENSP00000301785.5; ENSG00000214753.4.
DR GeneID; 221092; -.
DR KEGG; hsa:221092; -.
DR MANE-Select; ENST00000301785.7; ENSP00000301785.5; NM_001079559.3; NP_001073027.1.
DR UCSC; uc001nuw.4; human.
DR CTD; 221092; -.
DR DisGeNET; 221092; -.
DR GeneCards; HNRNPUL2; -.
DR HGNC; HGNC:25451; HNRNPUL2.
DR HPA; ENSG00000214753; Low tissue specificity.
DR neXtProt; NX_Q1KMD3; -.
DR OpenTargets; ENSG00000214753; -.
DR PharmGKB; PA162391554; -.
DR VEuPathDB; HostDB:ENSG00000214753; -.
DR eggNOG; KOG2242; Eukaryota.
DR GeneTree; ENSGT00940000160376; -.
DR HOGENOM; CLU_012140_1_0_1; -.
DR InParanoid; Q1KMD3; -.
DR OMA; DSRGLKM; -.
DR OrthoDB; 778148at2759; -.
DR PhylomeDB; Q1KMD3; -.
DR TreeFam; TF317301; -.
DR PathwayCommons; Q1KMD3; -.
DR SignaLink; Q1KMD3; -.
DR BioGRID-ORCS; 221092; 50 hits in 1082 CRISPR screens.
DR GenomeRNAi; 221092; -.
DR Pharos; Q1KMD3; Tbio.
DR PRO; PR:Q1KMD3; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q1KMD3; protein.
DR Bgee; ENSG00000214753; Expressed in middle temporal gyrus and 180 other tissues.
DR Genevisible; Q1KMD3; HS.
DR GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR CDD; cd12884; SPRY_hnRNP; 1.
DR Gene3D; 1.10.720.30; -; 1.
DR Gene3D; 2.60.120.920; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR001870; B30.2/SPRY.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR003034; SAP_dom.
DR InterPro; IPR036361; SAP_dom_sf.
DR InterPro; IPR003877; SPRY_dom.
DR InterPro; IPR035778; SPRY_hnRNP_U.
DR Pfam; PF02037; SAP; 1.
DR Pfam; PF00622; SPRY; 1.
DR SMART; SM00513; SAP; 1.
DR SMART; SM00449; SPRY; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF68906; SSF68906; 1.
DR PROSITE; PS50188; B302_SPRY; 1.
DR PROSITE; PS50800; SAP; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Methylation; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..747
FT /note="Heterogeneous nuclear ribonucleoprotein U-like
FT protein 2"
FT /id="PRO_0000278142"
FT DOMAIN 3..37
FT /note="SAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00186"
FT DOMAIN 226..419
FT /note="B30.2/SPRY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00548"
FT REGION 40..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 627..666
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 72..98
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..234
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 627..641
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 161
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q00PI9"
FT MOD_RES 165
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q00PI9"
FT MOD_RES 168
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q00PI9"
FT MOD_RES 185
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q00PI9"
FT MOD_RES 188
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q00PI9"
FT MOD_RES 226
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q00PI9"
FT MOD_RES 228
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|Ref.3"
FT MOD_RES 656
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0000250|UniProtKB:Q00PI9"
FT MOD_RES 684
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0000250|UniProtKB:Q00PI9"
FT MOD_RES 738
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 747
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
SQ SEQUENCE 747 AA; 85105 MW; 7EAAC2F598330AAA CRC64;
MEVKRLKVTE LRSELQRRGL DSRGLKVDLA QRLQEALDAE MLEDEAGGGG AGPGGACKAE
PRPVAASGGG PGGDEEEDEE EEEEDEEALL EDEDEEPPPA QALGQAAQPP PEPPEAAAME
AAAEPDASEK PAEATAGSGG VNGGEEQGLG KREEDEPEER SGDETPGSEV PGDKAAEEQG
DDQDSEKSKP AGSDGERRGV KRQRDEKDEH GRAYYEFREE AYHSRSKSPL PPEEEAKDEE
EDQTLVNLDT YTSDLHFQVS KDRYGGQPLF SEKFPTLWSG ARSTYGVTKG KVCFEAKVTQ
NLPMKEGCTE VSLLRVGWSV DFSRPQLGED EFSYGFDGRG LKAENGQFEE FGQTFGENDV
IGCFANFETE EVELSFSKNG EDLGVAFWIS KDSLADRALL PHVLCKNCVV ELNFGQKEEP
FFPPPEEFVF IHAVPVEERV RTAVPPKTIE ECEVILMVGL PGSGKTQWAL KYAKENPEKR
YNVLGAETVL NQMRMKGLEE PEMDPKSRDL LVQQASQCLS KLVQIASRTK RNFILDQCNV
YNSGQRRKLL LFKTFSRKVV VVVPNEEDWK KRLELRKEVE GDDVPESIML EMKANFSLPE
KCDYMDEVTY GELEKEEAQP IVTKYKEEAR KLLPPSEKRT NRRNNRNKRN RQNRSRGQGY
VGGQRRGYDN RAYGQQYWGQ PGNRGGYRNF YDRYRGDYDR FYGRDYEYNR YRDYYRQYNR
DWQSYYYHHP QDRDRYYRNY YGYQGYR
