ID   HNH95_VIBCE             Reviewed;         205 AA.
AC   P0DV99;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Retron Vc95 putative HNH endonuclease {ECO:0000303|PubMed:33157039};
DE   AltName: Full=ORF205 {ECO:0000303|PubMed:10564503};
DE   AltName: Full=TIGR02646 family protein {ECO:0000305};
GN   ORFNames=Ga0059260_01847, KV36_09040 {ECO:0000303|Ref.1};
OS   Vibrio cholerae serotype O1 biovar El Tor.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=686;
RN   [1] {ECO:0000312|EMBL:KFZ34333.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MAK676;
RA   Kritzky A., Cheldyshova N.B., Kulshan T.A., Krasnov Y.M., Cherkasov A.V.,
RA   Smirnova N.I.;
RT   "Vibrio cholerae O1 biovar El Tor MAK676 whole genome shotgun sequence.";
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   IDENTIFICATION.
RC   STRAIN=O139;
RX   PubMed=10564503; DOI=10.1046/j.1365-2958.1999.01531.x;
RA   Shimamoto T., Kobayashi M., Tsuchiya T., Shinoda S., Kawakami H.,
RA   Inouye S., Inouye M.;
RT   "A retroelement in Vibrio cholerae.";
RL   Mol. Microbiol. 34:631-632(1999).
RN   [3]
RP   FUNCTION IN ANTIVIRAL DEFENSE, AND IDENTIFICATION AS A RETRON.
RC   STRAIN=MAK676;
RX   PubMed=33157039; DOI=10.1016/j.cell.2020.09.065;
RA   Millman A., Bernheim A., Stokar-Avihail A., Fedorenko T., Voichek M.,
RA   Leavitt A., Oppenheimer-Shaanan Y., Sorek R.;
RT   "Bacterial Retrons Function In Anti-Phage Defense.";
RL   Cell 183:1551-1561(2020).
CC   -!- FUNCTION: Putative HNH endonuclease component of antiviral defense
CC       system retron Vc95, composed of a non-coding RNA (ncRNA), a reverse
CC       transcriptase (RT), a probable ATP-binding protein and this protein.
CC       Expression of retron Vc95 confers protection against bacteriophages T2,
CC       T4 and T6. At multiplicity of infection (MOI) of 0.02 cultures slow
CC       growth when infected with T4 but do not collapse, at MOI 2 cultures
CC       enter growth stasis. {ECO:0000269|PubMed:33157039}.
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DR   EMBL; JRBD01000006; KFZ34333.1; -; Genomic_DNA.
PE   1: Evidence at protein level;
KW   Antiviral defense; Endonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..205
FT                   /note="Retron Vc95 putative HNH endonuclease"
FT                   /id="PRO_0000456029"
SQ   SEQUENCE   205 AA;  23884 MW;  F359CA5238D68EB8 CRC64;
     MIPLSHNNTP TELENYVKLK GQSLTIQDFS AHDFQGVKKI VRDRLHTLQG ELCVYCEKKY
     SVDEMQVEHI KPKSGRNAQP NLCFTYSNYA VSCIQENRKT QTCGQKKKDN ILFIEPTSPS
     CNSHFSLDTD GFINPRGFKN RKEKHSIQTT IDMLGLNKPH LQLERKKQIE RLIYILKATK
     HNRHELTNKF IKSGNFKYIL RELTM