ID   HNH83_ECOLX             Reviewed;         258 AA.
AC   P0DV93;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Retron Ec83 putative HNH endonuclease {ECO:0000303|PubMed:33157039};
DE   AltName: Full=TIGR02646 family protein {ECO:0000305};
GN   ORFNames=Ga0124318_11283;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1] {ECO:0000312|EMBL:CXYK01000012}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=05-2753;
RA   Hur Y.J.;
RL   Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000312|EMBL:Z12832}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
RC   STRAIN=Clinical strain 161;
RX   PubMed=1282191; DOI=10.1111/j.1365-2958.1992.tb01788.x;
RA   Lim D.;
RT   "Structure and biosynthesis of unbranched multicopy single-stranded DNA by
RT   reverse transcriptase in a clinical Escherichia coli isolate.";
RL   Mol. Microbiol. 6:3531-3542(1992).
RN   [3]
RP   FUNCTION IN ANTIVIRAL DEFENSE, AND IDENTIFICATION AS A RETRON.
RC   STRAIN=05-2753;
RX   PubMed=33157039; DOI=10.1016/j.cell.2020.09.065;
RA   Millman A., Bernheim A., Stokar-Avihail A., Fedorenko T., Voichek M.,
RA   Leavitt A., Oppenheimer-Shaanan Y., Sorek R.;
RT   "Bacterial Retrons Function In Anti-Phage Defense.";
RL   Cell 183:1551-1561(2020).
CC   -!- FUNCTION: Putative HNH endonuclease component of antiviral defense
CC       system retron Ec83, composed of a non-coding RNA (ncRNA), a reverse
CC       transcriptase (RT), a probable ATPase and this protein. Expression of
CC       retron Ec78 confers protection against bacteriophage T2, T4 and T6. At
CC       multiplicity of infection (MOI) of 0.02 cultures slow growth when
CC       infected with T4 but do not collapse, at MOI 2 cultures enter growth
CC       stasis. {ECO:0000269|PubMed:33157039}.
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DR   EMBL; CXYK01000012; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; Z12832; -; NOT_ANNOTATED_CDS; Genomic_DNA.
PE   1: Evidence at protein level;
KW   Antiviral defense; Endonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..258
FT                   /note="Retron Ec83 putative HNH endonuclease"
FT                   /id="PRO_0000456028"
SQ   SEQUENCE   258 AA;  29964 MW;  04417CFA012EA4AA CRC64;
     MKRINKTAED QFLINFKAQN PNGTWDEFRN HEQGILYKRL KQHICNDQMY LCAYCEIDLD
     RENEHEIKVE HFKSKSGSLP GGSNWHLEWS NLLAVCLGGT NTGDDFELPA NLSCDSYKSH
     YEDKNKINDK DWTGKILLPL TLPDAHNFFT FEKVTGKLLP NESYCNTISI DGKPAAETLS
     IVTKTIEVLN LNCSRLNNAR RKLLFHFNNC ARERNLRKLH NLLLQWNQGE PKFFQTTRDI
     IIRDDRICQG LLNGTIRY