ID   HLGB_STAA8              Reviewed;         325 AA.
AC   Q2FVK1;
DT   14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Gamma-hemolysin component B;
DE   AltName: Full=H-gamma-1;
DE   AltName: Full=H-gamma-I;
DE   AltName: Full=Leukocidin f subunit;
DE   Flags: Precursor;
GN   Name=hlgB; OrderedLocusNames=SAOUHSC_02710;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND INDUCTION.
RC   STRAIN=RN4220;
RX   PubMed=20472795; DOI=10.1128/jb.01452-09;
RA   Sibbald M.J., Winter T., van der Kooi-Pol M.M., Buist G., Tsompanidou E.,
RA   Bosma T., Schafer T., Ohlsen K., Hecker M., Antelmann H., Engelmann S.,
RA   van Dijl J.M.;
RT   "Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in
RT   Staphylococcus aureus.";
RL   J. Bacteriol. 192:3788-3800(2010).
CC   -!- FUNCTION: Toxin that seems to act by forming pores in the membrane of
CC       the cell. Has a hemolytic and a leucotoxic activity (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Toxicity requires sequential binding and synergistic
CC       association of a class S and a class F component which form
CC       heterooligomeric complexes. HlgB (class F) associates with either hlgA
CC       thus forming an AB toxin or with hlgC thus forming a CB toxin (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20472795}.
CC   -!- INDUCTION: Less protein is secreted in a secG mutant (at protein
CC       level). {ECO:0000269|PubMed:20472795}.
CC   -!- SIMILARITY: Belongs to the aerolysin family. {ECO:0000305}.
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DR   EMBL; CP000253; ABD31718.1; -; Genomic_DNA.
DR   RefSeq; WP_000783428.1; NZ_LS483365.1.
DR   RefSeq; YP_501172.1; NC_007795.1.
DR   AlphaFoldDB; Q2FVK1; -.
DR   SMR; Q2FVK1; -.
DR   STRING; 1280.SAXN108_2677; -.
DR   EnsemblBacteria; ABD31718; ABD31718; SAOUHSC_02710.
DR   GeneID; 3919729; -.
DR   KEGG; sao:SAOUHSC_02710; -.
DR   PATRIC; fig|93061.5.peg.2454; -.
DR   eggNOG; ENOG50348U0; Bacteria.
DR   HOGENOM; CLU_055394_0_1_9; -.
DR   OMA; QNAKTHT; -.
DR   PRO; PR:Q2FVK1; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR003963; Bi-component_toxin_staph.
DR   InterPro; IPR016183; Leukocidin/Hemolysin_toxin.
DR   InterPro; IPR036435; Leukocidin/porin_MspA_sf.
DR   Pfam; PF07968; Leukocidin; 1.
DR   PRINTS; PR01468; BICOMPNTOXIN.
DR   SUPFAM; SSF56959; SSF56959; 1.
DR   TIGRFAMs; TIGR01002; hlyII; 1.
PE   1: Evidence at protein level;
KW   Cytolysis; Hemolysis; Reference proteome; Secreted; Signal; Toxin;
KW   Virulence.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..325
FT                   /note="Gamma-hemolysin component B"
FT                   /id="PRO_0000414601"
SQ   SEQUENCE   325 AA;  36711 MW;  082999EB9339B6A0 CRC64;
     MKMNKLVKSS VATSMALLLL SGTANAEGKI TPVSVKKVDD KVTLYKTTAT ADSDKFKISQ
     ILTFNFIKDK SYDKDTLVLK ATGNINSGFV KPNPNDYDFS KLYWGAKYNV SISSQSNDSV
     NVVDYAPKNQ NEEFQVQNTL GYTFGGDISI SNGLSGGLNG NTAFSETINY KQESYRTTLS
     RNTNYKNVGW GVEAHKIMNN GWGPYGRDSF HPTYGNELFL AGRQSSAYAG QNFIAQHQMP
     LLSRSNFNPE FLSVLSHRQD GAKKSKITVT YQREMDLYQI RWNGFYWAGA NYKNFKTRTF
     KSTYEIDWEN HKVKLLDTKE TENNK