HKT15_ORYSJ
ID HKT15_ORYSJ Reviewed; 554 AA.
AC Q0JNB6; A0A8F4N2R0; B9EVS1; Q3YAF4; Q8LQB2;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Cation transporter HKT1;5 {ECO:0000303|PubMed:16809061};
DE Short=OsHKT1;5 {ECO:0000303|PubMed:16809061};
DE AltName: Full=Cation transporter HKT8 {ECO:0000303|PubMed:12795699};
DE Short=OsHKT8 {ECO:0000303|PubMed:12795699};
GN Name=HKT1;5 {ECO:0000303|PubMed:16809061};
GN Synonyms=HKT8 {ECO:0000303|PubMed:12795699}, SKC1 {ECO:0000303|Ref.1};
GN OrderedLocusNames=Os01g0307500 {ECO:0000312|EMBL:BAS71759.1},
GN LOC_Os01g20160 {ECO:0000305};
GN ORFNames=OsJ_01471 {ECO:0000312|EMBL:EEE54420.1},
GN OSJNBb0022N24.25 {ECO:0000312|EMBL:BAB93392.1},
GN OSJNOa173H09.1 {ECO:0000312|EMBL:BAD88380.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Cho C., Kim K.H., Ahn E.-K., Park H., Choi M.-S., Chun J., Seo M.-S.,
RA Jin M., Kim D.-Y.;
RT "Selection of salt-tolerant silage rice through in vitro screening and
RT saltol QTL analysis.";
RL Submitted (MAY-2020) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NOMENCLATURE.
RX PubMed=12795699; DOI=10.1046/j.1365-313x.2003.01764.x;
RA Garciadeblas B., Senn M.E., Banuelos M.A., Rodriguez-Navarro A.;
RT "Sodium transport and HKT transporters: the rice model.";
RL Plant J. 34:788-801(2003).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16809061; DOI=10.1016/j.tplants.2006.06.001;
RA Platten J.D., Cotsaftis O., Berthomieu P., Bohnert H., Davenport R.J.,
RA Fairbairn D.J., Horie T., Leigh R.A., Lin H.X., Luan S., Maeser P.,
RA Pantoja O., Rodriguez-Navarro A., Schachtman D.P., Schroeder J.I.,
RA Sentenac H., Uozumi N., Very A.A., Zhu J.K., Dennis E.S., Tester M.;
RT "Nomenclature for HKT transporters, key determinants of plant salinity
RT tolerance.";
RL Trends Plant Sci. 11:372-374(2006).
RN [9]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=28488420; DOI=10.1111/tpj.13595;
RA Kobayashi N.I., Yamaji N., Yamamoto H., Okubo K., Ueno H., Costa A.,
RA Tanoi K., Matsumura H., Fujii-Kashino M., Horiuchi T., Nayef M.A.,
RA Shabala S., An G., Ma J.F., Horie T.;
RT "OsHKT1;5 mediates Na+ exclusion in the vasculature to protect leaf blades
RT and reproductive tissues from salt toxicity in rice.";
RL Plant J. 91:657-670(2017).
CC -!- FUNCTION: Sodium transporter that under salt stress mediates sodium
CC exclusion in the phloem to prevent sodium transfer to young leaf blades
CC and reproductive tissues. {ECO:0000269|PubMed:28488420}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Na(+)(in) = Na(+)(out); Xref=Rhea:RHEA:34963,
CC ChEBI:CHEBI:29101; Evidence={ECO:0000269|PubMed:28488420};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:28488420};
CC Multi-pass membrane protein {ECO:0000255}. Note=Localizes to the plasma
CC membrane. {ECO:0000269|PubMed:28488420}.
CC -!- TISSUE SPECIFICITY: In roots, expressed in cells next to the protoxylem
CC and the metaxylem I (PubMed:28488420). In basal nodes, expressed in the
CC phloem of some diffuse vascular bundles (PubMed:28488420).
CC {ECO:0000269|PubMed:28488420}.
CC -!- DOMAIN: HKT transporters are proposed to contain 4 pore-forming regions
CC enclosed by transmembrane segments with each containing a potassium
CC channel-like selectivity filter motif. {ECO:0000305}.
CC -!- MISCELLANEOUS: Substitution of Pro-140, Arg-184, His-332 and Leu-395 in
CC salt-tolerant cultivars indica Nona Bokra and Pokkali confers enhanced
CC sodium transport activity.
CC -!- SIMILARITY: Belongs to the TrkH potassium transport family. HKT (TC
CC 2.A.38.3) subfamily. {ECO:0000305}.
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DR EMBL; MT495788; QXE97982.1; -; Genomic_DNA.
DR EMBL; AP003567; BAB93392.1; -; Genomic_DNA.
DR EMBL; AP006856; BAD88380.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF04762.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS71759.1; -; Genomic_DNA.
DR EMBL; CM000138; EEE54420.1; -; Genomic_DNA.
DR RefSeq; XP_015631953.1; XM_015776467.1.
DR AlphaFoldDB; Q0JNB6; -.
DR STRING; 4530.OS01T0307500-01; -.
DR PaxDb; Q0JNB6; -.
DR PRIDE; Q0JNB6; -.
DR EnsemblPlants; Os01t0307500-01; Os01t0307500-01; Os01g0307500.
DR GeneID; 4327757; -.
DR Gramene; Os01t0307500-01; Os01t0307500-01; Os01g0307500.
DR KEGG; osa:4327757; -.
DR eggNOG; KOG1341; Eukaryota.
DR HOGENOM; CLU_008384_2_0_1; -.
DR InParanoid; Q0JNB6; -.
DR OMA; MPHILLG; -.
DR OrthoDB; 385384at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000007752; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q0JNB6; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProt.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR003445; Cat_transpt.
DR Pfam; PF02386; TrkH; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion transport; Membrane; Reference proteome; Sodium;
KW Sodium transport; Stress response; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..554
FT /note="Cation transporter HKT1;5"
FT /id="PRO_0000070472"
FT TOPO_DOM 1..25
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 26..46
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..207
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..230
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 301..341
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 403..423
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 424..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 444..464
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 518..538
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 539..554
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 127..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 554 AA; 60218 MW; 5433B2BB030F2ACB CRC64;
MSSLDATTPR YDEFKRIYHL FLFHAHPFWL QLLYFLFISL LGFLMLKALP MKTSMVPRPM
DLDLIFTSVS ATTVSSMVAV EMESFSNSQL LLITLLMLLG GEVFTSILGL YFTNAKYSSK
MIATLPDDDD HGGSGKPPPP TTSPSSTLVE LELAPPMDVV VVNPTTTATT HDEVELGLGR
RNKRGCTCTT THTSSSSSAS KTTTTRLLMF VVMGYHAVVH VAGYTAIVVY LSAVGGAGAV
VAGKGISAHT FAIFTVVSTF ANCGFVPTNE GMVSFRSFPG LLLLVMPHVL LGNTLFPVFL
RLAIAALERV TGWPELGELL IRRRRGGGEG YHHLLPSSRT RFLALTVAVL VVAQLALFCA
MEWGSDGLRG LTAGQKLVGA LFMAVNSRHS GEMVLDLSTV SSAVVVLYVV MMYLPPYTTF
VPVQDKHQQT GAQSGQEGSS SSSIWQKLLM SPLSCLAIFI VVICITERRQ IADDPINYSV
LNIVVEVISA YGNVGFSTGY SCARQVRPDG SCRDLWVGFS GKWSKQGKLT LMAVMFYGRL
KKFSLHGGQA WKIE
