ID   HBOH_MARN8              Reviewed;         700 AA.
AC   A1TWS1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=D-(-)-3-hydroxybutyrate oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HB-oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HBOH {ECO:0000255|HAMAP-Rule:MF_01906};
DE            EC=3.1.1.22 {ECO:0000255|HAMAP-Rule:MF_01906};
DE   Flags: Precursor;
GN   OrderedLocusNames=Maqu_0083;
OS   Marinobacter nauticus (strain ATCC 700491 / DSM 11845 / VT8) (Marinobacter
OS   aquaeolei).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Marinobacteraceae; Marinobacter.
OX   NCBI_TaxID=351348;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700491 / DSM 11845 / VT8;
RX   PubMed=21335390; DOI=10.1128/aem.01866-10;
RA   Singer E., Webb E.A., Nelson W.C., Heidelberg J.F., Ivanova N., Pati A.,
RA   Edwards K.J.;
RT   "Genomic potential of Marinobacter aquaeolei, a biogeochemical
RT   'opportunitroph'.";
RL   Appl. Environ. Microbiol. 77:2763-2771(2011).
CC   -!- FUNCTION: Participates in the degradation of poly-3-hydroxybutyrate
CC       (PHB). It works downstream of poly(3-hydroxybutyrate) depolymerase,
CC       hydrolyzing D(-)-3-hydroxybutyrate oligomers of various length (3HB-
CC       oligomers) into 3HB-monomers. {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxybutanoate dimer + H2O = 2 (R)-3-hydroxybutanoate +
CC         H(+); Xref=Rhea:RHEA:10172, ChEBI:CHEBI:10979, ChEBI:CHEBI:10983,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378; EC=3.1.1.22;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01906};
CC   -!- PATHWAY: Lipid metabolism; butanoate metabolism. {ECO:0000255|HAMAP-
CC       Rule:MF_01906}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- SIMILARITY: Belongs to the D-(-)-3-hydroxybutyrate oligomer hydrolase
CC       family. {ECO:0000255|HAMAP-Rule:MF_01906}.
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DR   EMBL; CP000514; ABM17190.1; -; Genomic_DNA.
DR   RefSeq; WP_011783663.1; NC_008740.1.
DR   AlphaFoldDB; A1TWS1; -.
DR   STRING; 351348.Maqu_0083; -.
DR   ESTHER; marhv-hboh; OHBut_olig_hydro_put.
DR   EnsemblBacteria; ABM17190; ABM17190; Maqu_0083.
DR   KEGG; maq:Maqu_0083; -.
DR   eggNOG; ENOG502Z8QU; Bacteria.
DR   HOGENOM; CLU_420258_0_0_6; -.
DR   OMA; NPEKDWG; -.
DR   OrthoDB; 865015at2; -.
DR   UniPathway; UPA00863; -.
DR   Proteomes; UP000000998; Chromosome.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   GO; GO:0047989; F:hydroxybutyrate-dimer hydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_01906; 3HBOH; 1.
DR   InterPro; IPR016582; OHBut_olig_hydro_put.
DR   Pfam; PF10605; 3HBOH; 1.
DR   PIRSF; PIRSF011409; HObutyrate_olig_hydrol; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
FT   CHAIN           22..700
FT                   /note="D-(-)-3-hydroxybutyrate oligomer hydrolase"
FT                   /id="PRO_5000208712"
FT   ACT_SITE        293
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
SQ   SEQUENCE   700 AA;  74222 MW;  FF517F646F96C11A CRC64;
     MKRMMLCTLI GAATVTLTAC KKDEVFNSLP DFLVSDVRQF DYDGNTDDLL TAGLGPEGLA
     SATPPAFVDA LNPTPAELRR LAIYNNYRAL VDTAPGGGYG SLFGPAVGNA SGRIPGQEFI
     AMMQVPGATV PVTVMAQVPD SFDPDFPCMV TAPSSGSRGI YGAIGTAGEW GLKKGCAVVY
     TDKGTGTGAH NLATNTAQSV NGTLTMEPEE ALFRADLDAQ SRDDFNSQWP DRFAWKHAHS
     KQNPEADWGR HVLQSIEFGF YMLNTLHGRE LGNGETLRTI NPRNTLVIAS SVSNGGGASV
     RAAEQDEKGL IDGVAVSEPN VNPAVDRSFS IRQGNGPALT EHSRSLLDYT TALAVYQGCA
     NQAPGIRDLA PLNGMFNPPA TGQNICHSLF NKGLVSGATK DDWALDAQRI LNEDFGVQPE
     QNLLAPVHFG LAVAQSIAMT YANAYGRAGV EDRVCGLSLA ATGSAGAVVP LAPAAEASLF
     AASNGIPPSA GVNLVYDNAE GQPTSLAVSA SPSSSQVDYG LDALLCLRSL AEGRDVVTGE
     PLEGAQAELH EAISRGISAV RASGDLQRKP TVFVTGRADA ILPINHTSRP YVGLNQAVEG
     RHSNLRYYEI LNAHHLDVLN GFPGIGDRYV PLHHYYFQAL DLMWARLTDK QPLPPSQVVR
     TVPRGSLTTP LTLANLPPIQ SEPAEADRIV FTGNQLQIPD